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Peroxiredoxin I and II inhibit H2O2-induced cell death in MCF-7 cell lines.
J Cell Biochem. 2007 Jul 01; 101(4):1038-45.JC

Abstract

Apoptosis is known to be induced by direct oxidative damage due to oxygen-free radicals or hydrogen peroxide or by their generation in cells by the actions of injurious agents. Together with glutathione peroxidase and catalase, peroxiredoxin (Prx) enzymes play an important role in eliminating peroxides generated during metabolism. We investigated the role of Prx enzymes during cellular response to oxidative stress. Using Prx isoforms-specific antibodies, we investigated the presence of Prx isoforms by immunoblot analysis in cell lysates of the MCF-7 breast cancer cell line. Treatment of MCF-7 with hydrogen peroxide (H2O2) resulted in the dose-dependent expressions of Prx I and II at the protein and mRNA levels. To investigate the physiologic relevance of the Prx I and II expressions induced by H2O2, we compared the survivals of MCF10A normal breast cell line and MCF-7 breast cancer cell line following exposure to H2O2. The treatment of MCF10A with H2O2 resulted in rapid cell death, whereas MCF-7 was resistant to H2O2. In addition, we found that Prx I and II transfection enabled MCF10A cells to resist H2O2-induced cell death. These findings suggest that Prx I and II have important functions as inhibitors of cell death during cellular response to oxidative stress.

Authors+Show Affiliations

Cancer Research Institute, Seoul National University, Jongno-Gu, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17163455

Citation

Bae, Ji-Yeon, et al. "Peroxiredoxin I and II Inhibit H2O2-induced Cell Death in MCF-7 Cell Lines." Journal of Cellular Biochemistry, vol. 101, no. 4, 2007, pp. 1038-45.
Bae JY, Ahn SJ, Han W, et al. Peroxiredoxin I and II inhibit H2O2-induced cell death in MCF-7 cell lines. J Cell Biochem. 2007;101(4):1038-45.
Bae, J. Y., Ahn, S. J., Han, W., & Noh, D. Y. (2007). Peroxiredoxin I and II inhibit H2O2-induced cell death in MCF-7 cell lines. Journal of Cellular Biochemistry, 101(4), 1038-45.
Bae JY, et al. Peroxiredoxin I and II Inhibit H2O2-induced Cell Death in MCF-7 Cell Lines. J Cell Biochem. 2007 Jul 1;101(4):1038-45. PubMed PMID: 17163455.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Peroxiredoxin I and II inhibit H2O2-induced cell death in MCF-7 cell lines. AU - Bae,Ji-Yeon, AU - Ahn,Soo-Jung, AU - Han,Wonshik, AU - Noh,Dong-Young, PY - 2006/12/14/pubmed PY - 2007/9/12/medline PY - 2006/12/14/entrez SP - 1038 EP - 45 JF - Journal of cellular biochemistry JO - J Cell Biochem VL - 101 IS - 4 N2 - Apoptosis is known to be induced by direct oxidative damage due to oxygen-free radicals or hydrogen peroxide or by their generation in cells by the actions of injurious agents. Together with glutathione peroxidase and catalase, peroxiredoxin (Prx) enzymes play an important role in eliminating peroxides generated during metabolism. We investigated the role of Prx enzymes during cellular response to oxidative stress. Using Prx isoforms-specific antibodies, we investigated the presence of Prx isoforms by immunoblot analysis in cell lysates of the MCF-7 breast cancer cell line. Treatment of MCF-7 with hydrogen peroxide (H2O2) resulted in the dose-dependent expressions of Prx I and II at the protein and mRNA levels. To investigate the physiologic relevance of the Prx I and II expressions induced by H2O2, we compared the survivals of MCF10A normal breast cell line and MCF-7 breast cancer cell line following exposure to H2O2. The treatment of MCF10A with H2O2 resulted in rapid cell death, whereas MCF-7 was resistant to H2O2. In addition, we found that Prx I and II transfection enabled MCF10A cells to resist H2O2-induced cell death. These findings suggest that Prx I and II have important functions as inhibitors of cell death during cellular response to oxidative stress. SN - 0730-2312 UR - https://www.unboundmedicine.com/medline/citation/17163455/Peroxiredoxin_I_and_II_inhibit_H2O2_induced_cell_death_in_MCF_7_cell_lines_ L2 - https://doi.org/10.1002/jcb.21155 DB - PRIME DP - Unbound Medicine ER -