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Salicylic acid-mediated innate immunity in Arabidopsis is regulated by SIZ1 SUMO E3 ligase.
Plant J. 2007 Jan; 49(1):79-90.PJ

Abstract

Reversible modifications of target proteins by small ubiquitin-like modifier (SUMO) proteins are involved in many cellular processes in yeast and animals. Yet little is known about the function of sumoylation in plants. Here, we show that the SIZ1 gene, which encodes an Arabidopsis SUMO E3 ligase, regulates innate immunity. Mutant siz1 plants exhibit constitutive systemic-acquired resistance (SAR) characterized by elevated accumulation of salicylic acid (SA), increased expression of pathogenesis-related (PR) genes, and increased resistance to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000. Transfer of the NahG gene to siz1 plants results in reversal of these phenotypes back to wild-type. Analyses of the double mutants, npr1 siz1, pad4 siz1 and ndr1 siz1 revealed that SIZ1 controls SA signalling. SIZ1 interacts epistatically with PAD4 to regulate PR expression and disease resistance. Consistent with these observations, siz1 plants exhibited enhanced resistance to Pst DC3000 expressing avrRps4, a bacterial avirulence determinant that responds to the EDS1/PAD4-dependent TIR-NBS-type R gene. In contrast, siz1 plants were not resistant to Pst DC3000 expressing avrRpm1, a bacterial avirulence determinant that responds to the NDR1-dependent CC-NBS-type R gene. Jasmonic acid (JA)-induced PDF1.2 expression and susceptibility to Botrytis cinerea were unaltered in siz1 plants. Taken together, these results demonstrate that SIZ1 is required for SA and PAD4-mediated R gene signalling, which in turn confers innate immunity in Arabidopsis.

Authors+Show Affiliations

Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnolgy Research Center and Environmental Biotechnology National Core Research Center, Graduate School of Gyeongsang National University, Jinju, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17163880

Citation

Lee, Jiyoung, et al. "Salicylic Acid-mediated Innate Immunity in Arabidopsis Is Regulated By SIZ1 SUMO E3 Ligase." The Plant Journal : for Cell and Molecular Biology, vol. 49, no. 1, 2007, pp. 79-90.
Lee J, Nam J, Park HC, et al. Salicylic acid-mediated innate immunity in Arabidopsis is regulated by SIZ1 SUMO E3 ligase. Plant J. 2007;49(1):79-90.
Lee, J., Nam, J., Park, H. C., Na, G., Miura, K., Jin, J. B., Yoo, C. Y., Baek, D., Kim, D. H., Jeong, J. C., Kim, D., Lee, S. Y., Salt, D. E., Mengiste, T., Gong, Q., Ma, S., Bohnert, H. J., Kwak, S. S., Bressan, R. A., ... Yun, D. J. (2007). Salicylic acid-mediated innate immunity in Arabidopsis is regulated by SIZ1 SUMO E3 ligase. The Plant Journal : for Cell and Molecular Biology, 49(1), 79-90.
Lee J, et al. Salicylic Acid-mediated Innate Immunity in Arabidopsis Is Regulated By SIZ1 SUMO E3 Ligase. Plant J. 2007;49(1):79-90. PubMed PMID: 17163880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Salicylic acid-mediated innate immunity in Arabidopsis is regulated by SIZ1 SUMO E3 ligase. AU - Lee,Jiyoung, AU - Nam,Jaesung, AU - Park,Hyeong Cheol, AU - Na,Gunnam, AU - Miura,Kenji, AU - Jin,Jing Bo, AU - Yoo,Chan Yul, AU - Baek,Dongwon, AU - Kim,Doh Hoon, AU - Jeong,Jae Cheol, AU - Kim,Donggiun, AU - Lee,Sang Yeol, AU - Salt,David E, AU - Mengiste,Tesfaye, AU - Gong,Qingqiu, AU - Ma,Shisong, AU - Bohnert,Hans J, AU - Kwak,Sang-Soo, AU - Bressan,Ray A, AU - Hasegawa,Paul M, AU - Yun,Dae-Jin, Y1 - 2006/12/06/ PY - 2006/12/14/pubmed PY - 2007/4/7/medline PY - 2006/12/14/entrez SP - 79 EP - 90 JF - The Plant journal : for cell and molecular biology JO - Plant J. VL - 49 IS - 1 N2 - Reversible modifications of target proteins by small ubiquitin-like modifier (SUMO) proteins are involved in many cellular processes in yeast and animals. Yet little is known about the function of sumoylation in plants. Here, we show that the SIZ1 gene, which encodes an Arabidopsis SUMO E3 ligase, regulates innate immunity. Mutant siz1 plants exhibit constitutive systemic-acquired resistance (SAR) characterized by elevated accumulation of salicylic acid (SA), increased expression of pathogenesis-related (PR) genes, and increased resistance to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000. Transfer of the NahG gene to siz1 plants results in reversal of these phenotypes back to wild-type. Analyses of the double mutants, npr1 siz1, pad4 siz1 and ndr1 siz1 revealed that SIZ1 controls SA signalling. SIZ1 interacts epistatically with PAD4 to regulate PR expression and disease resistance. Consistent with these observations, siz1 plants exhibited enhanced resistance to Pst DC3000 expressing avrRps4, a bacterial avirulence determinant that responds to the EDS1/PAD4-dependent TIR-NBS-type R gene. In contrast, siz1 plants were not resistant to Pst DC3000 expressing avrRpm1, a bacterial avirulence determinant that responds to the NDR1-dependent CC-NBS-type R gene. Jasmonic acid (JA)-induced PDF1.2 expression and susceptibility to Botrytis cinerea were unaltered in siz1 plants. Taken together, these results demonstrate that SIZ1 is required for SA and PAD4-mediated R gene signalling, which in turn confers innate immunity in Arabidopsis. SN - 0960-7412 UR - https://www.unboundmedicine.com/medline/citation/17163880/Salicylic_acid_mediated_innate_immunity_in_Arabidopsis_is_regulated_by_SIZ1_SUMO_E3_ligase_ L2 - https://doi.org/10.1111/j.1365-313X.2006.02947.x DB - PRIME DP - Unbound Medicine ER -