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Relevance of the recombinant lipid transfer protein of Hevea brasiliensis: IgE-binding reactivity in fruit-allergic adults.
Ann Allergy Asthma Immunol. 2006 Nov; 97(5):643-9.AA

Abstract

BACKGROUND

Lipid transfer proteins (LTPs) are relevant allergens in certain plants. The role of the LTP of Hevea brasiliensis in the latex-fruit syndrome is widely unknown.

OBJECTIVE

To study IgE reactivity with recombinant Hevea LTP in sera of fruit-allergic adults with and without natural rubber latex (NRL) allergy.

METHODS

An LTP-specific complementary DNA of H brasiliensis leaves was amplified, subcloned into the pMAL expression system, and analyzed. The recombinant protein was coupled to ImmunoCAP, and the IgE-binding properties were studied in sera of 10 NRL-allergic patients without symptoms to fruit and 48 atopic patients with fruit allergy. Eleven of these 48 patients were also allergic to NRL, 14 displayed sensitization to NRL without symptoms on NRL exposure so far, and 23 had neither symptoms nor IgE antibodies to NRL.

RESULTS

After expression in Escherichia coli, a soluble maltose-binding protein-rHev b 12 fusion protein was isolated and coupled to ImmunoCAP to determine rHev b 12 specific IgE reactivity. rHev b 12 specific IgE binding was found in 3 fruit-allergic patients with NRL sensitization (0.68, 0.88, and 0.96 kU/L) and in 3 fruit-allergic patients without NRL sensitization (1.58, 2.25, and 2.27 kU/L). The remaining 52 serum samples and all maltose-binding protein control test results were negative (< 0.35 kU/L).

CONCLUSIONS

In these patients, rHev b 12 specific IgE reactivity seems to result from common cross-reactive epitopes with some of the fruit LTPs tested and underscores only an involvement in co-recognition. No clinical relevance of IgE binding to the LTP of H brasiliensis in association with NRL allergy was detected.

Authors+Show Affiliations

Abteilung Molekulare Genetik, Berufsgenossenschaftliches Forschungsin-Institut für Arbeitsmedizin (BGFA), Institut der Ruhr-Universität Bochum, Bochum, Germany. rihs@bgfa.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17165273

Citation

Rihs, Hans-Peter, et al. "Relevance of the Recombinant Lipid Transfer Protein of Hevea Brasiliensis: IgE-binding Reactivity in Fruit-allergic Adults." Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology, vol. 97, no. 5, 2006, pp. 643-9.
Rihs HP, Ruëff F, Lundberg M, et al. Relevance of the recombinant lipid transfer protein of Hevea brasiliensis: IgE-binding reactivity in fruit-allergic adults. Ann Allergy Asthma Immunol. 2006;97(5):643-9.
Rihs, H. P., Ruëff, F., Lundberg, M., Rozynek, P., Barber, D., Scheurer, S., Cisteró-Bahima, A., Brüning, T., & Raulf-Heimsoth, M. (2006). Relevance of the recombinant lipid transfer protein of Hevea brasiliensis: IgE-binding reactivity in fruit-allergic adults. Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology, 97(5), 643-9.
Rihs HP, et al. Relevance of the Recombinant Lipid Transfer Protein of Hevea Brasiliensis: IgE-binding Reactivity in Fruit-allergic Adults. Ann Allergy Asthma Immunol. 2006;97(5):643-9. PubMed PMID: 17165273.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relevance of the recombinant lipid transfer protein of Hevea brasiliensis: IgE-binding reactivity in fruit-allergic adults. AU - Rihs,Hans-Peter, AU - Ruëff,Franziska, AU - Lundberg,Maria, AU - Rozynek,Peter, AU - Barber,Domingo, AU - Scheurer,Stephan, AU - Cisteró-Bahima,Anna, AU - Brüning,Thomas, AU - Raulf-Heimsoth,Monika, PY - 2006/12/15/pubmed PY - 2007/1/18/medline PY - 2006/12/15/entrez SP - 643 EP - 9 JF - Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology JO - Ann Allergy Asthma Immunol VL - 97 IS - 5 N2 - BACKGROUND: Lipid transfer proteins (LTPs) are relevant allergens in certain plants. The role of the LTP of Hevea brasiliensis in the latex-fruit syndrome is widely unknown. OBJECTIVE: To study IgE reactivity with recombinant Hevea LTP in sera of fruit-allergic adults with and without natural rubber latex (NRL) allergy. METHODS: An LTP-specific complementary DNA of H brasiliensis leaves was amplified, subcloned into the pMAL expression system, and analyzed. The recombinant protein was coupled to ImmunoCAP, and the IgE-binding properties were studied in sera of 10 NRL-allergic patients without symptoms to fruit and 48 atopic patients with fruit allergy. Eleven of these 48 patients were also allergic to NRL, 14 displayed sensitization to NRL without symptoms on NRL exposure so far, and 23 had neither symptoms nor IgE antibodies to NRL. RESULTS: After expression in Escherichia coli, a soluble maltose-binding protein-rHev b 12 fusion protein was isolated and coupled to ImmunoCAP to determine rHev b 12 specific IgE reactivity. rHev b 12 specific IgE binding was found in 3 fruit-allergic patients with NRL sensitization (0.68, 0.88, and 0.96 kU/L) and in 3 fruit-allergic patients without NRL sensitization (1.58, 2.25, and 2.27 kU/L). The remaining 52 serum samples and all maltose-binding protein control test results were negative (< 0.35 kU/L). CONCLUSIONS: In these patients, rHev b 12 specific IgE reactivity seems to result from common cross-reactive epitopes with some of the fruit LTPs tested and underscores only an involvement in co-recognition. No clinical relevance of IgE binding to the LTP of H brasiliensis in association with NRL allergy was detected. SN - 1081-1206 UR - https://www.unboundmedicine.com/medline/citation/17165273/Relevance_of_the_recombinant_lipid_transfer_protein_of_Hevea_brasiliensis:_IgE_binding_reactivity_in_fruit_allergic_adults_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1081-1206(10)61094-0 DB - PRIME DP - Unbound Medicine ER -