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Absorption spectra of human fetal and adult oxyhemoglobin, de-oxyhemoglobin, carboxyhemoglobin, and methemoglobin.
Clin Chem. 1991 Sep; 37(9):1633-8.CC

Abstract

We determined the millimolar absorptivities of the four clinically relevant derivatives of fetal and adult human hemoglobin in the visible and near-infrared spectral range (450-1000 nm). As expected, spectral absorption curves of similar shape were found, but the small differences between fetal and adult hemoglobin absorptivity were important enough that they should be taken into account in multicomponent analysis of hemoglobin derivatives. Common pulse oximeters, however, involving light of 660 and 940 nm, are so insensitive to the presence of fetal hemoglobin that they can be used safely in neonates. The error in pulse oximetry caused by the presence of carboxyhemoglobin is insubstantial, but methemoglobin gives either an understimation or an overestimation at high or low oxygen saturation, respectively, the turning point being near 70% saturation.

Authors+Show Affiliations

Zijlstra WG
Department of Physiology, University of Groningen, The Netherlands.
Buursma A
No affiliation info available
Meeuwsen-van der Roest WP
No affiliation info available

MeSH

AdultCarboxyhemoglobinFetal HemoglobinHemoglobinsHumansInfant, NewbornMethemoglobinOximetryOxyhemoglobinsSpectrophotometry, Infrared

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1716537

Citation

Zijlstra, W G., et al. "Absorption Spectra of Human Fetal and Adult Oxyhemoglobin, De-oxyhemoglobin, Carboxyhemoglobin, and Methemoglobin." Clinical Chemistry, vol. 37, no. 9, 1991, pp. 1633-8.
Zijlstra WG, Buursma A, Meeuwsen-van der Roest WP. Absorption spectra of human fetal and adult oxyhemoglobin, de-oxyhemoglobin, carboxyhemoglobin, and methemoglobin. Clin Chem. 1991;37(9):1633-8.
Zijlstra, W. G., Buursma, A., & Meeuwsen-van der Roest, W. P. (1991). Absorption spectra of human fetal and adult oxyhemoglobin, de-oxyhemoglobin, carboxyhemoglobin, and methemoglobin. Clinical Chemistry, 37(9), 1633-8.
Zijlstra WG, Buursma A, Meeuwsen-van der Roest WP. Absorption Spectra of Human Fetal and Adult Oxyhemoglobin, De-oxyhemoglobin, Carboxyhemoglobin, and Methemoglobin. Clin Chem. 1991;37(9):1633-8. PubMed PMID: 1716537.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Absorption spectra of human fetal and adult oxyhemoglobin, de-oxyhemoglobin, carboxyhemoglobin, and methemoglobin. AU - Zijlstra,W G, AU - Buursma,A, AU - Meeuwsen-van der Roest,W P, PY - 1991/9/1/pubmed PY - 1991/9/1/medline PY - 1991/9/1/entrez SP - 1633 EP - 8 JF - Clinical chemistry JO - Clin Chem VL - 37 IS - 9 N2 - We determined the millimolar absorptivities of the four clinically relevant derivatives of fetal and adult human hemoglobin in the visible and near-infrared spectral range (450-1000 nm). As expected, spectral absorption curves of similar shape were found, but the small differences between fetal and adult hemoglobin absorptivity were important enough that they should be taken into account in multicomponent analysis of hemoglobin derivatives. Common pulse oximeters, however, involving light of 660 and 940 nm, are so insensitive to the presence of fetal hemoglobin that they can be used safely in neonates. The error in pulse oximetry caused by the presence of carboxyhemoglobin is insubstantial, but methemoglobin gives either an understimation or an overestimation at high or low oxygen saturation, respectively, the turning point being near 70% saturation. SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/1716537/Absorption_spectra_of_human_fetal_and_adult_oxyhemoglobin_de_oxyhemoglobin_carboxyhemoglobin_and_methemoglobin_ DB - PRIME DP - Unbound Medicine ER -