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Stem cells from umbilical cord blood differentiate into myotubes and express dystrophin in vitro only after exposure to in vivo muscle environment.
Biol Cell. 2007 Apr; 99(4):185-96.BC

Abstract

BACKGROUND INFORMATION

Duchenne muscular dystrophy is a disease characterized by progressive and irreversible muscle degeneration for which there is no therapy. HUCB (human umbilical cord blood) has been considered as an important source of haematopoietic and mesenchymal stem cells, each having been shown to differentiate into distinct cell types. However, it remains unclear if these cells are able to differentiate into muscle cells.

RESULTS

We have showed that stem cells from HUCB did not differentiate into myotubes or express dystrophin when cultured in muscle-conditioned medium or with human muscle cells. However, delivery of GFP (green fluorescent protein)-transduced mononucleated cells from HUCB, which comprises both haematopoietic and mesenchymal populations, into quadriceps muscle of mdx (mouse dystrophy X-chromosome linked) mice resulted in the expression of human myogenic markers. After recovery of these cells from mdx muscle and in vitro cultivation, they were able to fuse and form GFP-positive myotubes that expressed dystrophin.

CONCLUSIONS

These results indicate that chemical factors and cell-to-cell contact provided by in vitro conditions were not enough to trigger the differentiation of stem cells into muscle cells. Nevertheless, we showed that the HUCB-derived stem cells were capable of acquiring a muscle phenotype after exposure to an in vivo muscle environment, which was required to activate the differentiation programme.

Authors+Show Affiliations

Human Genome Research Center, Department of Genetics and Evolutionary Biology, University of Sao Paulo, Sao Paulo, SP, Brazil. vanunes@ib.usp.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17166095

Citation

Nunes, Viviane A., et al. "Stem Cells From Umbilical Cord Blood Differentiate Into Myotubes and Express Dystrophin in Vitro Only After Exposure to in Vivo Muscle Environment." Biology of the Cell, vol. 99, no. 4, 2007, pp. 185-96.
Nunes VA, Cavaçana N, Canovas M, et al. Stem cells from umbilical cord blood differentiate into myotubes and express dystrophin in vitro only after exposure to in vivo muscle environment. Biol Cell. 2007;99(4):185-96.
Nunes, V. A., Cavaçana, N., Canovas, M., Strauss, B. E., & Zatz, M. (2007). Stem cells from umbilical cord blood differentiate into myotubes and express dystrophin in vitro only after exposure to in vivo muscle environment. Biology of the Cell, 99(4), 185-96.
Nunes VA, et al. Stem Cells From Umbilical Cord Blood Differentiate Into Myotubes and Express Dystrophin in Vitro Only After Exposure to in Vivo Muscle Environment. Biol Cell. 2007;99(4):185-96. PubMed PMID: 17166095.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stem cells from umbilical cord blood differentiate into myotubes and express dystrophin in vitro only after exposure to in vivo muscle environment. AU - Nunes,Viviane A, AU - Cavaçana,Natale, AU - Canovas,Martha, AU - Strauss,Bryan E, AU - Zatz,Mayana, PY - 2006/12/15/pubmed PY - 2007/5/30/medline PY - 2006/12/15/entrez SP - 185 EP - 96 JF - Biology of the cell JO - Biol. Cell VL - 99 IS - 4 N2 - BACKGROUND INFORMATION: Duchenne muscular dystrophy is a disease characterized by progressive and irreversible muscle degeneration for which there is no therapy. HUCB (human umbilical cord blood) has been considered as an important source of haematopoietic and mesenchymal stem cells, each having been shown to differentiate into distinct cell types. However, it remains unclear if these cells are able to differentiate into muscle cells. RESULTS: We have showed that stem cells from HUCB did not differentiate into myotubes or express dystrophin when cultured in muscle-conditioned medium or with human muscle cells. However, delivery of GFP (green fluorescent protein)-transduced mononucleated cells from HUCB, which comprises both haematopoietic and mesenchymal populations, into quadriceps muscle of mdx (mouse dystrophy X-chromosome linked) mice resulted in the expression of human myogenic markers. After recovery of these cells from mdx muscle and in vitro cultivation, they were able to fuse and form GFP-positive myotubes that expressed dystrophin. CONCLUSIONS: These results indicate that chemical factors and cell-to-cell contact provided by in vitro conditions were not enough to trigger the differentiation of stem cells into muscle cells. Nevertheless, we showed that the HUCB-derived stem cells were capable of acquiring a muscle phenotype after exposure to an in vivo muscle environment, which was required to activate the differentiation programme. SN - 0248-4900 UR - https://www.unboundmedicine.com/medline/citation/17166095/Stem_cells_from_umbilical_cord_blood_differentiate_into_myotubes_and_express_dystrophin_in_vitro_only_after_exposure_to_in_vivo_muscle_environment_ L2 - https://doi.org/10.1042/BC20060075 DB - PRIME DP - Unbound Medicine ER -