Tags

Type your tag names separated by a space and hit enter

The association of VP1 unique region protein in acute parvovirus B19 infection and anti-phospholipid antibody production.
Clin Chim Acta. 2007 Mar; 378(1-2):59-65.CC

Abstract

BACKGROUND

Previous studies have postulated a connection between human parvovirus B19 (B19) infection and anti-phospholipid antibodies (aPL). Recently, the phospholipase domain of B19 has been linked to B19-VP1 unique region (VP1u). To elucidate the roles of VP1u in B19 infection and aPL production, the major reactivity of anti-B19-VP1u, anti-cardiolipin antibody (aCL), and anti-beta2-glycoprotein I (beta2GPI) antibody was evaluated.

METHODS

Sera from 102 clinically suspected cases of B19 infection were analyzed by nested PCR and ELISA. Humoral responses of anti-B19-VP1u and anti-B19-VP1uD175A IgM/IgG antibodies, aCL and the anti-beta2GPI antibody were assessed by Western blot and ELISA. Absorption experiments were also performed to determine the binding specificity of immunoglobulins to B19-VP1u, CL and beta2GPI.

RESULTS

Sera from patients with the diagnostic pattern DNA+/IgM+/IgG+ had a high frequency (57%) for recognition of CL and beta2GPI. Furthermore, adsorption experiments were performed by adding purified B19-VP1u, which partially suppressed the reactivity of anti-B19VP1u to CL and beta2GPI.

CONCLUSIONS

Serum from patients with acute B19 infection has a high frequency in recognition of CL and beta2GPI, and the phospholipase domain observed in the B19-VP1u may have contributed to the production of aPL. These findings may provide a clue for understanding the roles of B19-VP1u in B19 infection and aPL production.

Authors+Show Affiliations

Institute of Biochemistry and Biotechnology, Chung Shan Medical University, and Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17169353

Citation

Tzang, Bor-Show, et al. "The Association of VP1 Unique Region Protein in Acute Parvovirus B19 Infection and Anti-phospholipid Antibody Production." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 378, no. 1-2, 2007, pp. 59-65.
Tzang BS, Tsay GJ, Lee YJ, et al. The association of VP1 unique region protein in acute parvovirus B19 infection and anti-phospholipid antibody production. Clin Chim Acta. 2007;378(1-2):59-65.
Tzang, B. S., Tsay, G. J., Lee, Y. J., Li, C., Sun, Y. S., & Hsu, T. C. (2007). The association of VP1 unique region protein in acute parvovirus B19 infection and anti-phospholipid antibody production. Clinica Chimica Acta; International Journal of Clinical Chemistry, 378(1-2), 59-65.
Tzang BS, et al. The Association of VP1 Unique Region Protein in Acute Parvovirus B19 Infection and Anti-phospholipid Antibody Production. Clin Chim Acta. 2007;378(1-2):59-65. PubMed PMID: 17169353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The association of VP1 unique region protein in acute parvovirus B19 infection and anti-phospholipid antibody production. AU - Tzang,Bor-Show, AU - Tsay,Gregory J, AU - Lee,Yi-Ju, AU - Li,Chin, AU - Sun,Yu-Shu, AU - Hsu,Tsai-Ching, Y1 - 2006/11/10/ PY - 2006/05/26/received PY - 2006/10/20/revised PY - 2006/10/23/accepted PY - 2006/12/16/pubmed PY - 2007/4/18/medline PY - 2006/12/16/entrez SP - 59 EP - 65 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin Chim Acta VL - 378 IS - 1-2 N2 - BACKGROUND: Previous studies have postulated a connection between human parvovirus B19 (B19) infection and anti-phospholipid antibodies (aPL). Recently, the phospholipase domain of B19 has been linked to B19-VP1 unique region (VP1u). To elucidate the roles of VP1u in B19 infection and aPL production, the major reactivity of anti-B19-VP1u, anti-cardiolipin antibody (aCL), and anti-beta2-glycoprotein I (beta2GPI) antibody was evaluated. METHODS: Sera from 102 clinically suspected cases of B19 infection were analyzed by nested PCR and ELISA. Humoral responses of anti-B19-VP1u and anti-B19-VP1uD175A IgM/IgG antibodies, aCL and the anti-beta2GPI antibody were assessed by Western blot and ELISA. Absorption experiments were also performed to determine the binding specificity of immunoglobulins to B19-VP1u, CL and beta2GPI. RESULTS: Sera from patients with the diagnostic pattern DNA+/IgM+/IgG+ had a high frequency (57%) for recognition of CL and beta2GPI. Furthermore, adsorption experiments were performed by adding purified B19-VP1u, which partially suppressed the reactivity of anti-B19VP1u to CL and beta2GPI. CONCLUSIONS: Serum from patients with acute B19 infection has a high frequency in recognition of CL and beta2GPI, and the phospholipase domain observed in the B19-VP1u may have contributed to the production of aPL. These findings may provide a clue for understanding the roles of B19-VP1u in B19 infection and aPL production. SN - 0009-8981 UR - https://www.unboundmedicine.com/medline/citation/17169353/The_association_of_VP1_unique_region_protein_in_acute_parvovirus_B19_infection_and_anti_phospholipid_antibody_production_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-8981(06)00707-8 DB - PRIME DP - Unbound Medicine ER -