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Examination of induced sputum in the diagnosis of Pneumocystis carinii pneumonia.
Cytopathology. 1991; 2(2):61-6.C

Abstract

The results of the examination of sputum induced by the inhalation of nebulized hypertonic saline in the diagnosis of Pneumocystis carinii pneumonia (PCP) are presented. In suspected cases of PCP in patients who were either HIV antibody positive or were receiving immunosuppressive therapy, 46 induced sputum specimens were stained using both Grocott's modified Gomori methenamine silver nitrate (GMS) and immunofluorescence staining. In 12 specimens P. carinii cysts were detected by both methods, in four specimens by GMS staining only and in five specimens by immunofluorescence only. The sensitivity of induced sputum examination in the detection of P. carinii cysts was increased by using both of these staining methods on each sputum specimen and the need for more invasive methods of diagnosis was reduced.

Authors+Show Affiliations

Department of Medicine, United Medical School, London.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1717023

Citation

Carmichael, A, et al. "Examination of Induced Sputum in the Diagnosis of Pneumocystis Carinii Pneumonia." Cytopathology : Official Journal of the British Society for Clinical Cytology, vol. 2, no. 2, 1991, pp. 61-6.
Carmichael A, Bateman N, Nayagam M. Examination of induced sputum in the diagnosis of Pneumocystis carinii pneumonia. Cytopathology. 1991;2(2):61-6.
Carmichael, A., Bateman, N., & Nayagam, M. (1991). Examination of induced sputum in the diagnosis of Pneumocystis carinii pneumonia. Cytopathology : Official Journal of the British Society for Clinical Cytology, 2(2), 61-6.
Carmichael A, Bateman N, Nayagam M. Examination of Induced Sputum in the Diagnosis of Pneumocystis Carinii Pneumonia. Cytopathology. 1991;2(2):61-6. PubMed PMID: 1717023.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Examination of induced sputum in the diagnosis of Pneumocystis carinii pneumonia. AU - Carmichael,A, AU - Bateman,N, AU - Nayagam,M, PY - 1991/1/1/pubmed PY - 1991/1/1/medline PY - 1991/1/1/entrez SP - 61 EP - 6 JF - Cytopathology : official journal of the British Society for Clinical Cytology JO - Cytopathology VL - 2 IS - 2 N2 - The results of the examination of sputum induced by the inhalation of nebulized hypertonic saline in the diagnosis of Pneumocystis carinii pneumonia (PCP) are presented. In suspected cases of PCP in patients who were either HIV antibody positive or were receiving immunosuppressive therapy, 46 induced sputum specimens were stained using both Grocott's modified Gomori methenamine silver nitrate (GMS) and immunofluorescence staining. In 12 specimens P. carinii cysts were detected by both methods, in four specimens by GMS staining only and in five specimens by immunofluorescence only. The sensitivity of induced sputum examination in the detection of P. carinii cysts was increased by using both of these staining methods on each sputum specimen and the need for more invasive methods of diagnosis was reduced. SN - 0956-5507 UR - https://www.unboundmedicine.com/medline/citation/1717023/Examination_of_induced_sputum_in_the_diagnosis_of_Pneumocystis_carinii_pneumonia_ DB - PRIME DP - Unbound Medicine ER -