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Structural assignment of disialylated biantennary N-glycan isomers derivatized with 2-aminopyridine using negative-ion multistage tandem mass spectral matching.
Rapid Commun Mass Spectrom. 2007; 21(2):212-8.RC

Abstract

To investigate the possibility of structural assignment based on negative-ion multistage tandem mass (MS(n)) spectral matching, four isomers of disialylated biantennary N-glycans (alpha2-6 and/or alpha2-3 linked sialic acid on alpha1-6 and alpha1-3 antennae) derivatized with 2-aminopyridine (PA) were analyzed by employing high-performance liquid chromatography/electrospray ionization linear ion trap time-of-flight mass spectrometry (HPLC/ESI-LIT-TOFMS), which uses helium gas for ion trapping and collision-induced dissociation (CID). It is shown that the MS(2) spectra derived from each precursor ion [M-2H](2-) are reproducible and useful for distinguishing the four isomers. Thus, they can be assigned by negative-ion MS(2) spectral matching based on correlation coefficients. In addition, MS(3) spectra derived from D-type fragment ions clearly differentiate the alpha2-3- or alpha2-6-linked sialic acid on the alpha1-6 antenna due to their characteristic spectral patterns. The C(4)-type fragment ions, which are produced from both the alpha1-6 and alpha1-3 antennae, show the characteristic MS(3) spectra reflecting alpha2-3- or alpha2-6- linkage type or a mixture of both types. Thus, the differentiation and assignment of these disialylated biantennary N-glycan isomers can also be supported with the MS(3) spectra of C(4)- and D-type ions.

Authors+Show Affiliations

Graduate School of Advanced Life Science, Hokkaido University, Sapporo 001-0021, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17171781

Citation

Ito, Hiroki, et al. "Structural Assignment of Disialylated Biantennary N-glycan Isomers Derivatized With 2-aminopyridine Using Negative-ion Multistage Tandem Mass Spectral Matching." Rapid Communications in Mass Spectrometry : RCM, vol. 21, no. 2, 2007, pp. 212-8.
Ito H, Yamada K, Deguchi K, et al. Structural assignment of disialylated biantennary N-glycan isomers derivatized with 2-aminopyridine using negative-ion multistage tandem mass spectral matching. Rapid Commun Mass Spectrom. 2007;21(2):212-8.
Ito, H., Yamada, K., Deguchi, K., Nakagawa, H., & Nishimura, S. (2007). Structural assignment of disialylated biantennary N-glycan isomers derivatized with 2-aminopyridine using negative-ion multistage tandem mass spectral matching. Rapid Communications in Mass Spectrometry : RCM, 21(2), 212-8.
Ito H, et al. Structural Assignment of Disialylated Biantennary N-glycan Isomers Derivatized With 2-aminopyridine Using Negative-ion Multistage Tandem Mass Spectral Matching. Rapid Commun Mass Spectrom. 2007;21(2):212-8. PubMed PMID: 17171781.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural assignment of disialylated biantennary N-glycan isomers derivatized with 2-aminopyridine using negative-ion multistage tandem mass spectral matching. AU - Ito,Hiroki, AU - Yamada,Kuriko, AU - Deguchi,Kisaburo, AU - Nakagawa,Hiroaki, AU - Nishimura,Shin-Ichiro, PY - 2006/12/16/pubmed PY - 2007/2/16/medline PY - 2006/12/16/entrez SP - 212 EP - 8 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 21 IS - 2 N2 - To investigate the possibility of structural assignment based on negative-ion multistage tandem mass (MS(n)) spectral matching, four isomers of disialylated biantennary N-glycans (alpha2-6 and/or alpha2-3 linked sialic acid on alpha1-6 and alpha1-3 antennae) derivatized with 2-aminopyridine (PA) were analyzed by employing high-performance liquid chromatography/electrospray ionization linear ion trap time-of-flight mass spectrometry (HPLC/ESI-LIT-TOFMS), which uses helium gas for ion trapping and collision-induced dissociation (CID). It is shown that the MS(2) spectra derived from each precursor ion [M-2H](2-) are reproducible and useful for distinguishing the four isomers. Thus, they can be assigned by negative-ion MS(2) spectral matching based on correlation coefficients. In addition, MS(3) spectra derived from D-type fragment ions clearly differentiate the alpha2-3- or alpha2-6-linked sialic acid on the alpha1-6 antenna due to their characteristic spectral patterns. The C(4)-type fragment ions, which are produced from both the alpha1-6 and alpha1-3 antennae, show the characteristic MS(3) spectra reflecting alpha2-3- or alpha2-6- linkage type or a mixture of both types. Thus, the differentiation and assignment of these disialylated biantennary N-glycan isomers can also be supported with the MS(3) spectra of C(4)- and D-type ions. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/17171781/Structural_assignment_of_disialylated_biantennary_N_glycan_isomers_derivatized_with_2_aminopyridine_using_negative_ion_multistage_tandem_mass_spectral_matching_ DB - PRIME DP - Unbound Medicine ER -