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Rapid diagnosis of scrub typhus in rural Thailand using polymerase chain reaction.
Am J Trop Med Hyg. 2006 Dec; 75(6):1099-102.AJ

Abstract

The aim of this study was to evaluate the use of polymerase chain reaction (PCR) amplification of the O. tsutsugamushi 16S rRNA gene for the diagnosis of scrub typhus in rural Thailand. A prospective study of acute febrile illness in Udon Thani, northeast Thailand, identified 183 patients as having scrub typhus on the basis of immunofluorescent antibody testing (IFA) of paired sera. A further 366 febrile patients admitted concurrently with a range of other diagnoses acted as negative controls. Diagnostic sensitivity and specificity of 16S rRNA PCR was 44.8% and 99.7%, respectively, compared with IFA. PCR positivity was related to duration of symptoms and presence of eschar (P < 0.001, both cases). PCR using primers to amplify a fragment of the 56-kd gene had a sensitivity and specificity of 29.0% and 99.2%, respectively. PCR has a high specificity but low sensitivity for the rapid diagnosis of scrub typhus in this endemic setting.

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Authors+Show Affiliations

Sonthayanon P
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. piengchan@tropmedres.ac
Chierakul W
No affiliation info available
Wuthiekanun V
No affiliation info available
Blacksell SD
No affiliation info available
Pimda K
No affiliation info available
Suputtamongkol Y
No affiliation info available
Pukrittayakamee S
No affiliation info available
White NJ
No affiliation info available
Day NP
No affiliation info available
Peacock SJ
No affiliation info available

MeSH

HumansOrientia tsutsugamushiPolymerase Chain ReactionRNA, BacterialRNA, Ribosomal, 16SRural PopulationScrub TyphusSensitivity and SpecificityThailand

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17172374

Citation

Sonthayanon, Piengchan, et al. "Rapid Diagnosis of Scrub Typhus in Rural Thailand Using Polymerase Chain Reaction." The American Journal of Tropical Medicine and Hygiene, vol. 75, no. 6, 2006, pp. 1099-102.
Sonthayanon P, Chierakul W, Wuthiekanun V, et al. Rapid diagnosis of scrub typhus in rural Thailand using polymerase chain reaction. Am J Trop Med Hyg. 2006;75(6):1099-102.
Sonthayanon, P., Chierakul, W., Wuthiekanun, V., Blacksell, S. D., Pimda, K., Suputtamongkol, Y., Pukrittayakamee, S., White, N. J., Day, N. P., & Peacock, S. J. (2006). Rapid diagnosis of scrub typhus in rural Thailand using polymerase chain reaction. The American Journal of Tropical Medicine and Hygiene, 75(6), 1099-102.
Sonthayanon P, et al. Rapid Diagnosis of Scrub Typhus in Rural Thailand Using Polymerase Chain Reaction. Am J Trop Med Hyg. 2006;75(6):1099-102. PubMed PMID: 17172374.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid diagnosis of scrub typhus in rural Thailand using polymerase chain reaction. AU - Sonthayanon,Piengchan, AU - Chierakul,Wirongrong, AU - Wuthiekanun,Vanaporn, AU - Blacksell,Stuart D, AU - Pimda,Kriangsak, AU - Suputtamongkol,Yupin, AU - Pukrittayakamee,Sasithon, AU - White,Nicholas J, AU - Day,Nicholas P, AU - Peacock,Sharon J, PY - 2006/12/19/pubmed PY - 2007/4/5/medline PY - 2006/12/19/entrez SP - 1099 EP - 102 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 75 IS - 6 N2 - The aim of this study was to evaluate the use of polymerase chain reaction (PCR) amplification of the O. tsutsugamushi 16S rRNA gene for the diagnosis of scrub typhus in rural Thailand. A prospective study of acute febrile illness in Udon Thani, northeast Thailand, identified 183 patients as having scrub typhus on the basis of immunofluorescent antibody testing (IFA) of paired sera. A further 366 febrile patients admitted concurrently with a range of other diagnoses acted as negative controls. Diagnostic sensitivity and specificity of 16S rRNA PCR was 44.8% and 99.7%, respectively, compared with IFA. PCR positivity was related to duration of symptoms and presence of eschar (P < 0.001, both cases). PCR using primers to amplify a fragment of the 56-kd gene had a sensitivity and specificity of 29.0% and 99.2%, respectively. PCR has a high specificity but low sensitivity for the rapid diagnosis of scrub typhus in this endemic setting. SN - 0002-9637 UR - https://www.unboundmedicine.com/medline/citation/17172374/Rapid_diagnosis_of_scrub_typhus_in_rural_Thailand_using_polymerase_chain_reaction_ L2 - http://www.ajtmh.org/cgi/pmidlookup?view=long&amp;pmid=17172374 DB - PRIME DP - Unbound Medicine ER -