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The temporal profile of genomic responses and protein synthesis in ischemic tolerance of the rat brain induced by repeated hyperbaric oxygen.
Brain Res. 2007 Jan 26; 1130(1):214-22.BR

Abstract

Repeated hyperbaric oxygen (HBO) exposure prior to ischemia has been reported to provide neuroprotection against ischemic brain injury. The present study examined the time course of neuroprotection of HBO (3.5 atmosphere absolute, 100% oxygen, 1 h for 5 consecutive days) and the changes of gene/protein expression in rats. First, at 6 h, 12 h, 24 h, and 72 h after HBO sessions, rats were subjected to forebrain ischemia (8 min). Histopathological examination of hippocampal CA1 neurons was done 7 days after ischemia. Second, temporal genomic responses and protein expression were examined at the same time points after HBO sessions without subjecting animals to ischemia. HBO significantly reduced loss of hippocampal CA1 neurons that normally follows transient forebrain ischemia when the last HBO session was 6 h, 12 h, or 24 h before ischemia (survived neurons 55%, 75%, and 53%, respectively), whereas if there was a 72-h delay before the ischemic insult, HBO was not protective (survived neurons only 6%). Statistical analysis on microarray data showed significant upregulation in 60 probe sets including 7 annotated genes (p75NTR, C/EBPdelta, CD74, Edg2, Trip10, Nrp1, and Igf2), whose time course expressions corresponded to HBO-induced neuroprotection. The protein levels of p75NTR, C/EBPdelta, and CD74 were significantly increased (maximum fold changes 2.9, 2.0, and 7.9, respectively). The results suggest that HBO-induced neuroprotection against ischemic injury has time window, protective at 6 h, 12 h and 24 h but not protective at 72 h. Although the precise interaction is to be determined, the genes/proteins relevant to neurotrophin and inflammatory-immune system may be involved in HBO-induced neuroprotection.

Authors+Show Affiliations

Department of Anesthesiology-Resuscitology, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi, 755-8505 Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17173873

Citation

Hirata, Takao, et al. "The Temporal Profile of Genomic Responses and Protein Synthesis in Ischemic Tolerance of the Rat Brain Induced By Repeated Hyperbaric Oxygen." Brain Research, vol. 1130, no. 1, 2007, pp. 214-22.
Hirata T, Cui YJ, Funakoshi T, et al. The temporal profile of genomic responses and protein synthesis in ischemic tolerance of the rat brain induced by repeated hyperbaric oxygen. Brain Res. 2007;1130(1):214-22.
Hirata, T., Cui, Y. J., Funakoshi, T., Mizukami, Y., Ishikawa, Y., Shibasaki, F., Matsumoto, M., & Sakabe, T. (2007). The temporal profile of genomic responses and protein synthesis in ischemic tolerance of the rat brain induced by repeated hyperbaric oxygen. Brain Research, 1130(1), 214-22.
Hirata T, et al. The Temporal Profile of Genomic Responses and Protein Synthesis in Ischemic Tolerance of the Rat Brain Induced By Repeated Hyperbaric Oxygen. Brain Res. 2007 Jan 26;1130(1):214-22. PubMed PMID: 17173873.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The temporal profile of genomic responses and protein synthesis in ischemic tolerance of the rat brain induced by repeated hyperbaric oxygen. AU - Hirata,Takao, AU - Cui,Ying Jun, AU - Funakoshi,Takeshi, AU - Mizukami,Yoichi, AU - Ishikawa,Yu-Ichiro, AU - Shibasaki,Futoshi, AU - Matsumoto,Mishiya, AU - Sakabe,Takefumi, Y1 - 2006/12/13/ PY - 2006/08/02/received PY - 2006/10/21/revised PY - 2006/10/23/accepted PY - 2006/12/19/pubmed PY - 2007/3/14/medline PY - 2006/12/19/entrez SP - 214 EP - 22 JF - Brain research JO - Brain Res. VL - 1130 IS - 1 N2 - Repeated hyperbaric oxygen (HBO) exposure prior to ischemia has been reported to provide neuroprotection against ischemic brain injury. The present study examined the time course of neuroprotection of HBO (3.5 atmosphere absolute, 100% oxygen, 1 h for 5 consecutive days) and the changes of gene/protein expression in rats. First, at 6 h, 12 h, 24 h, and 72 h after HBO sessions, rats were subjected to forebrain ischemia (8 min). Histopathological examination of hippocampal CA1 neurons was done 7 days after ischemia. Second, temporal genomic responses and protein expression were examined at the same time points after HBO sessions without subjecting animals to ischemia. HBO significantly reduced loss of hippocampal CA1 neurons that normally follows transient forebrain ischemia when the last HBO session was 6 h, 12 h, or 24 h before ischemia (survived neurons 55%, 75%, and 53%, respectively), whereas if there was a 72-h delay before the ischemic insult, HBO was not protective (survived neurons only 6%). Statistical analysis on microarray data showed significant upregulation in 60 probe sets including 7 annotated genes (p75NTR, C/EBPdelta, CD74, Edg2, Trip10, Nrp1, and Igf2), whose time course expressions corresponded to HBO-induced neuroprotection. The protein levels of p75NTR, C/EBPdelta, and CD74 were significantly increased (maximum fold changes 2.9, 2.0, and 7.9, respectively). The results suggest that HBO-induced neuroprotection against ischemic injury has time window, protective at 6 h, 12 h and 24 h but not protective at 72 h. Although the precise interaction is to be determined, the genes/proteins relevant to neurotrophin and inflammatory-immune system may be involved in HBO-induced neuroprotection. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/17173873/The_temporal_profile_of_genomic_responses_and_protein_synthesis_in_ischemic_tolerance_of_the_rat_brain_induced_by_repeated_hyperbaric_oxygen_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(06)03168-4 DB - PRIME DP - Unbound Medicine ER -