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Calcium-calmodulin is required for abscisic acid-induced antioxidant defense and functions both upstream and downstream of H2O2 production in leaves of maize (Zea mays) plants.
New Phytol. 2007; 173(1):27-38.NP

Abstract

* Using pharmacological and biochemical approaches, the role of calmodulin (CaM) and the relationship between CaM and hydrogen peroxide (H(2)O(2)) in abscisic acid (ABA)-induced antioxidant defense in leaves of maize (Zea mays) plants were investigated. * Treatment with ABA or H(2)O(2) led to significant increases in the concentration of cytosolic Ca(2+) in the protoplasts of mesophyll cells and in the expression of the calmodulin 1 (CaM1) gene and the content of CaM in leaves of maize plants, and enhanced the expression of the antioxidant genes superoxide dismutase 4 (SOD4), cytosolic ascorbate peroxidase (cAPX), and glutathione reductase 1 (GR1) and the activities of the chloroplastic and cytosolic antioxidant enzymes. The up-regulation of the antioxidant enzymes was almost completely blocked by pretreatments with two CaM antagonists. * Pretreatments with CaM antagonists almost completely inhibited ABA-induced H(2)O(2) production throughout ABA treatment, but pretreatment with an inhibitor or scavenger of reactive oxygen species (ROS) did not affect the initial increase in the contents of CaM induced by ABA. * Our results suggest that Ca(2+)-CaM is involved in ABA-induced antioxidant defense, and that cross-talk between Ca(2+)-CaM and H(2)O(2) plays a pivotal role in ABA signaling.

Authors+Show Affiliations

Hu X
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Jiang M
No affiliation info available
Zhang J
No affiliation info available
Zhang A
No affiliation info available
Lin F
No affiliation info available
Tan M
No affiliation info available

MeSH

Abscisic AcidAntioxidantsAscorbate PeroxidasesCalciumCalmodulinCytosolGene Expression Regulation, PlantGlutathione ReductaseHydrogen PeroxidePeroxidasesPlant Growth RegulatorsPlant LeavesPlant ProteinsProtoplastsReactive Oxygen SpeciesSignal TransductionSuperoxide DismutaseZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17176391

Citation

Hu, Xiuli, et al. "Calcium-calmodulin Is Required for Abscisic Acid-induced Antioxidant Defense and Functions Both Upstream and Downstream of H2O2 Production in Leaves of Maize (Zea Mays) Plants." The New Phytologist, vol. 173, no. 1, 2007, pp. 27-38.
Hu X, Jiang M, Zhang J, et al. Calcium-calmodulin is required for abscisic acid-induced antioxidant defense and functions both upstream and downstream of H2O2 production in leaves of maize (Zea mays) plants. New Phytol. 2007;173(1):27-38.
Hu, X., Jiang, M., Zhang, J., Zhang, A., Lin, F., & Tan, M. (2007). Calcium-calmodulin is required for abscisic acid-induced antioxidant defense and functions both upstream and downstream of H2O2 production in leaves of maize (Zea mays) plants. The New Phytologist, 173(1), 27-38.
Hu X, et al. Calcium-calmodulin Is Required for Abscisic Acid-induced Antioxidant Defense and Functions Both Upstream and Downstream of H2O2 Production in Leaves of Maize (Zea Mays) Plants. New Phytol. 2007;173(1):27-38. PubMed PMID: 17176391.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Calcium-calmodulin is required for abscisic acid-induced antioxidant defense and functions both upstream and downstream of H2O2 production in leaves of maize (Zea mays) plants. AU - Hu,Xiuli, AU - Jiang,Mingyi, AU - Zhang,Jianhua, AU - Zhang,Aying, AU - Lin,Fan, AU - Tan,Mingpu, PY - 2006/12/21/pubmed PY - 2007/3/6/medline PY - 2006/12/21/entrez SP - 27 EP - 38 JF - The New phytologist JO - New Phytol VL - 173 IS - 1 N2 - * Using pharmacological and biochemical approaches, the role of calmodulin (CaM) and the relationship between CaM and hydrogen peroxide (H(2)O(2)) in abscisic acid (ABA)-induced antioxidant defense in leaves of maize (Zea mays) plants were investigated. * Treatment with ABA or H(2)O(2) led to significant increases in the concentration of cytosolic Ca(2+) in the protoplasts of mesophyll cells and in the expression of the calmodulin 1 (CaM1) gene and the content of CaM in leaves of maize plants, and enhanced the expression of the antioxidant genes superoxide dismutase 4 (SOD4), cytosolic ascorbate peroxidase (cAPX), and glutathione reductase 1 (GR1) and the activities of the chloroplastic and cytosolic antioxidant enzymes. The up-regulation of the antioxidant enzymes was almost completely blocked by pretreatments with two CaM antagonists. * Pretreatments with CaM antagonists almost completely inhibited ABA-induced H(2)O(2) production throughout ABA treatment, but pretreatment with an inhibitor or scavenger of reactive oxygen species (ROS) did not affect the initial increase in the contents of CaM induced by ABA. * Our results suggest that Ca(2+)-CaM is involved in ABA-induced antioxidant defense, and that cross-talk between Ca(2+)-CaM and H(2)O(2) plays a pivotal role in ABA signaling. SN - 0028-646X UR - https://www.unboundmedicine.com/medline/citation/17176391/Calcium_calmodulin_is_required_for_abscisic_acid_induced_antioxidant_defense_and_functions_both_upstream_and_downstream_of_H2O2_production_in_leaves_of_maize__Zea_mays__plants_ L2 - https://doi.org/10.1111/j.1469-8137.2006.01888.x DB - PRIME DP - Unbound Medicine ER -