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Immunophenotype characteristics of peripheral blood mononuclear leukocytes of chronic idiopathic urticaria patients.
Invest Clin. 2006 Dec; 47(4):361-9.IC

Abstract

The pathogenesis of chronic idiopathic urticaria (CIU) is not completely understood although autoimmunity has been proposed. The aim of the study was to assess the expression of different leukocyte antigens, by flow cytometry, assaying total blood of 29 patients with CIU and of 20 sex and age matched controls. Moreover, we assessed soluble CD154 a marker of immune cell activation, predominantly memory T cells. When patients were divided depending an their response to the autologous serum skin test (ASST), three different groups were encountered: group 1 (n=11): with negative ASST-, group 2 (n=11): positive ASST (ASST+) with normal lymphocyte counts and group 3 (n=7): ASST+ with low lymphocyte counts (< 1500 cells/mm3). A significant increase in CD19+ percentage and not in the absolute count (P < 0.05) was observed in group 1 as compared to controls and to the other groups. In contrast, CD30+, CD45RO+ and CD4+/CD45RO+ percentages and biologically active soluble CD154 levels were significantly higher (P < 0.05) in group 3 as compared to group 1 or to controls. In ASST positive groups, CD45RO+ and CD4+/CD45RO+ positiveness correlates with wheal diameter. In conclusion, memory cells may play a role in these different types of patients and in understanding CIU pathogenesis.

Authors+Show Affiliations

Garmendia JV
Instituto de Inmunología, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela. garmmenj@ucv.ve
Zabaleta M
No affiliation info available
Aldrey O
No affiliation info available
Rivera H
No affiliation info available
De Sanctis JB
No affiliation info available
Bianco NE
No affiliation info available
Blanca I
No affiliation info available

MeSH

AdultAntigens, CDChronic DiseaseData Interpretation, StatisticalFemaleFlow CytometryHumansImmunologic MemoryImmunophenotypingLeukocyte CountLymphocyte CountMaleMiddle AgedSkin TestsT-LymphocytesUrticaria

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17176904

Citation

Garmendia, Jenny V., et al. "Immunophenotype Characteristics of Peripheral Blood Mononuclear Leukocytes of Chronic Idiopathic Urticaria Patients." Investigacion Clinica, vol. 47, no. 4, 2006, pp. 361-9.
Garmendia JV, Zabaleta M, Aldrey O, et al. Immunophenotype characteristics of peripheral blood mononuclear leukocytes of chronic idiopathic urticaria patients. Invest Clin. 2006;47(4):361-9.
Garmendia, J. V., Zabaleta, M., Aldrey, O., Rivera, H., De Sanctis, J. B., Bianco, N. E., & Blanca, I. (2006). Immunophenotype characteristics of peripheral blood mononuclear leukocytes of chronic idiopathic urticaria patients. Investigacion Clinica, 47(4), 361-9.
Garmendia JV, et al. Immunophenotype Characteristics of Peripheral Blood Mononuclear Leukocytes of Chronic Idiopathic Urticaria Patients. Invest Clin. 2006;47(4):361-9. PubMed PMID: 17176904.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunophenotype characteristics of peripheral blood mononuclear leukocytes of chronic idiopathic urticaria patients. AU - Garmendia,Jenny V, AU - Zabaleta,Mercedes, AU - Aldrey,Oscar, AU - Rivera,Henry, AU - De Sanctis,Juan B, AU - Bianco,Nicolás E, AU - Blanca,Isaac, PY - 2006/12/21/pubmed PY - 2007/2/13/medline PY - 2006/12/21/entrez SP - 361 EP - 9 JF - Investigacion clinica JO - Invest Clin VL - 47 IS - 4 N2 - The pathogenesis of chronic idiopathic urticaria (CIU) is not completely understood although autoimmunity has been proposed. The aim of the study was to assess the expression of different leukocyte antigens, by flow cytometry, assaying total blood of 29 patients with CIU and of 20 sex and age matched controls. Moreover, we assessed soluble CD154 a marker of immune cell activation, predominantly memory T cells. When patients were divided depending an their response to the autologous serum skin test (ASST), three different groups were encountered: group 1 (n=11): with negative ASST-, group 2 (n=11): positive ASST (ASST+) with normal lymphocyte counts and group 3 (n=7): ASST+ with low lymphocyte counts (< 1500 cells/mm3). A significant increase in CD19+ percentage and not in the absolute count (P < 0.05) was observed in group 1 as compared to controls and to the other groups. In contrast, CD30+, CD45RO+ and CD4+/CD45RO+ percentages and biologically active soluble CD154 levels were significantly higher (P < 0.05) in group 3 as compared to group 1 or to controls. In ASST positive groups, CD45RO+ and CD4+/CD45RO+ positiveness correlates with wheal diameter. In conclusion, memory cells may play a role in these different types of patients and in understanding CIU pathogenesis. SN - 0535-5133 UR - https://www.unboundmedicine.com/medline/citation/17176904/Immunophenotype_characteristics_of_peripheral_blood_mononuclear_leukocytes_of_chronic_idiopathic_urticaria_patients_ DB - PRIME DP - Unbound Medicine ER -