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Total flavonoids of Daphne genkwa root significantly inhibit the growth and metastasis of Lewis lung carcinoma in C57BL6 mice.
Int Immunopharmacol. 2007 Feb; 7(2):117-27.II

Abstract

Daphne genkwa root has been traditionally used as an effective remedy to treat various tumors. However, the active constituents for its antitumor potency have not been well documented. During the screening for antitumor constituents, it was found that the total flavonoids of D. genkwa root (TFDR) were responsible for the inhibition of tumor growth and metastasis. In this study, TFDR was investigated for its chemical composition and activities against tumor growth and metastasis. HPLC indicates that daphnodorin B, containing 42.79% of the total, represents the predominant constituent in TFDR. Treatment of LLC-bearing mice with TFDR evidently protected peripheral lymphocytes from tumor-induced reduction, and increased lymphocyte proliferation potential and cytolytic activity of NK, and inhibited tumor progression and metastasis either 7 days before, or simultaneous with, or 7 days after LLC transplantation. TFDR also suggested higher cytotoxicity to a number of tumor cell lines than that to normal human kidney cell K293. TFDR also induced an enhancement on peripheral release of TNF-alpha at doses between 25 and 75 mg/kg. These results indicated that TFDR inhibited tumor growth and metastasis by protecting host immunocyte viability and its proliferation potential, and selectively inhibiting tumor cell proliferation, and improving cytolytic activity of NK cells, and enhancing TNF release in LLC-bearing mice. Daphnodorin B and its analogues in TFDR are the active constituents in the roots of D. genkwa, contributing to the inhibition of tumor growth and metastasis.

Authors+Show Affiliations

Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou, China. yyzw@xznu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17178377

Citation

Zheng, Weifa, et al. "Total Flavonoids of Daphne Genkwa Root Significantly Inhibit the Growth and Metastasis of Lewis Lung Carcinoma in C57BL6 Mice." International Immunopharmacology, vol. 7, no. 2, 2007, pp. 117-27.
Zheng W, Gao X, Chen C, et al. Total flavonoids of Daphne genkwa root significantly inhibit the growth and metastasis of Lewis lung carcinoma in C57BL6 mice. Int Immunopharmacol. 2007;7(2):117-27.
Zheng, W., Gao, X., Chen, C., & Tan, R. (2007). Total flavonoids of Daphne genkwa root significantly inhibit the growth and metastasis of Lewis lung carcinoma in C57BL6 mice. International Immunopharmacology, 7(2), 117-27.
Zheng W, et al. Total Flavonoids of Daphne Genkwa Root Significantly Inhibit the Growth and Metastasis of Lewis Lung Carcinoma in C57BL6 Mice. Int Immunopharmacol. 2007;7(2):117-27. PubMed PMID: 17178377.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Total flavonoids of Daphne genkwa root significantly inhibit the growth and metastasis of Lewis lung carcinoma in C57BL6 mice. AU - Zheng,Weifa, AU - Gao,Xiaowen, AU - Chen,Caifa, AU - Tan,Renxiang, Y1 - 2006/08/04/ PY - 2006/05/20/received PY - 2006/06/19/revised PY - 2006/07/09/accepted PY - 2006/12/21/pubmed PY - 2007/3/22/medline PY - 2006/12/21/entrez SP - 117 EP - 27 JF - International immunopharmacology JO - Int Immunopharmacol VL - 7 IS - 2 N2 - Daphne genkwa root has been traditionally used as an effective remedy to treat various tumors. However, the active constituents for its antitumor potency have not been well documented. During the screening for antitumor constituents, it was found that the total flavonoids of D. genkwa root (TFDR) were responsible for the inhibition of tumor growth and metastasis. In this study, TFDR was investigated for its chemical composition and activities against tumor growth and metastasis. HPLC indicates that daphnodorin B, containing 42.79% of the total, represents the predominant constituent in TFDR. Treatment of LLC-bearing mice with TFDR evidently protected peripheral lymphocytes from tumor-induced reduction, and increased lymphocyte proliferation potential and cytolytic activity of NK, and inhibited tumor progression and metastasis either 7 days before, or simultaneous with, or 7 days after LLC transplantation. TFDR also suggested higher cytotoxicity to a number of tumor cell lines than that to normal human kidney cell K293. TFDR also induced an enhancement on peripheral release of TNF-alpha at doses between 25 and 75 mg/kg. These results indicated that TFDR inhibited tumor growth and metastasis by protecting host immunocyte viability and its proliferation potential, and selectively inhibiting tumor cell proliferation, and improving cytolytic activity of NK cells, and enhancing TNF release in LLC-bearing mice. Daphnodorin B and its analogues in TFDR are the active constituents in the roots of D. genkwa, contributing to the inhibition of tumor growth and metastasis. SN - 1567-5769 UR - https://www.unboundmedicine.com/medline/citation/17178377/Total_flavonoids_of_Daphne_genkwa_root_significantly_inhibit_the_growth_and_metastasis_of_Lewis_lung_carcinoma_in_C57BL6_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(06)00211-6 DB - PRIME DP - Unbound Medicine ER -