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Detection of fungal spores using a generic surface plasmon resonance immunoassay.
Biosens Bioelectron. 2007 May 15; 22(11):2724-9.BB

Abstract

This paper describes a biosensor-based method for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a mouse monoclonal antibody (Pst mAb8) and a SPR sensor for label-free detection of urediniospores from the model organism Puccinia striiformis f.sp. tritici (Pst). In the subtractive inhibition assay, urediniospores and Pst mAb8 were mixed, urediniospore-bound Pst mAb8 removed by centrifugation and the remaining Pst mAb8 quantified using the SPR sensor. Assay conditions were optimised and a detection limit of 3.1 x 10(5)urediniospores/ml was achieved. Spiked Pst samples were further examined in a background of a related spore and it was found that Pst detection was possible in this mixture. This study represent the first use of SPR technology for fungal spore detection as well as the first report of a successful biosensor-based detection strategy for Pst.

Authors+Show Affiliations

Danish Institute of Agricultural Sciences, Department of Integrated Pest Management, DK-4200 Slagelse, Denmark. psk@biocentrum.dtu.dk <psk@biocentrum.dtu.dk>No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17178456

Citation

Skottrup, Peter, et al. "Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay." Biosensors & Bioelectronics, vol. 22, no. 11, 2007, pp. 2724-9.
Skottrup P, Hearty S, Frøkiaer H, et al. Detection of fungal spores using a generic surface plasmon resonance immunoassay. Biosens Bioelectron. 2007;22(11):2724-9.
Skottrup, P., Hearty, S., Frøkiaer, H., Leonard, P., Hejgaard, J., O'Kennedy, R., Nicolaisen, M., & Justesen, A. F. (2007). Detection of fungal spores using a generic surface plasmon resonance immunoassay. Biosensors & Bioelectronics, 22(11), 2724-9.
Skottrup P, et al. Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay. Biosens Bioelectron. 2007 May 15;22(11):2724-9. PubMed PMID: 17178456.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of fungal spores using a generic surface plasmon resonance immunoassay. AU - Skottrup,Peter, AU - Hearty,Stephen, AU - Frøkiaer,Hanne, AU - Leonard,Paul, AU - Hejgaard,Jørn, AU - O'Kennedy,Richard, AU - Nicolaisen,Mogens, AU - Justesen,Annemarie F, Y1 - 2006/12/18/ PY - 2006/07/05/received PY - 2006/11/11/revised PY - 2006/11/15/accepted PY - 2006/12/21/pubmed PY - 2007/6/28/medline PY - 2006/12/21/entrez SP - 2724 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 11 N2 - This paper describes a biosensor-based method for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a mouse monoclonal antibody (Pst mAb8) and a SPR sensor for label-free detection of urediniospores from the model organism Puccinia striiformis f.sp. tritici (Pst). In the subtractive inhibition assay, urediniospores and Pst mAb8 were mixed, urediniospore-bound Pst mAb8 removed by centrifugation and the remaining Pst mAb8 quantified using the SPR sensor. Assay conditions were optimised and a detection limit of 3.1 x 10(5)urediniospores/ml was achieved. Spiked Pst samples were further examined in a background of a related spore and it was found that Pst detection was possible in this mixture. This study represent the first use of SPR technology for fungal spore detection as well as the first report of a successful biosensor-based detection strategy for Pst. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/17178456/Detection_of_fungal_spores_using_a_generic_surface_plasmon_resonance_immunoassay_ DB - PRIME DP - Unbound Medicine ER -