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A new method of standartization of health-promoting pomegranate fruit (Punica granatum) extract.
Georgian Med News. 2006 NovGM

Abstract

This study analyzes the major phenolic constituents of pomegranate fruit juice and pericarp feedstock, and dry extracts thereof, using high-performance liquid chromatography. Pomegranate pericarp was extracted with water (WE) and alcohol (AE) as solvents, and liquid extracts were subsequently freeze-dried. The results indicate that ellagitannins punicalagin A and punicalagin B are the major constituents in the primary pomegranate feedstock and in both types of extracts. Ellagic acid, a common botanical constituent that is currently used to standardize pomegranate extracts, as well as ellagitannin punicalin, were found to be only minor constituents. Total punicalagins (the sum of punicalagins A+B and punicalin) and ellagic acid content in the pomegranate fruit pericarp feedstock WE were 7,6+/-0,3% and 0,2+/-0,1% by dry weight, respectively, and in the AE feedstock 7,0+/-0,2 and 0,4+/-0,1%, respectively. Total phenolic content (the sum of punicalagins and ellagic acid) in the pomegranate WE and AE were 45,8+/-1,2% and 42,3+/-1,1%, respectively. The concentrations of ellagic acid in the pomegranate WE and AE were 0,8+/-0,2% and 3,9+/-0,2%, respectively. Total phenolics in fresh single-strength pomegranate whole fruit juice contained 2,216+/-70 mg/L (95% punicalagins), whereas commercial pomegranate juice that was purchased from local stores was 317+/-13 mg/L (70% punicalagins). Our results strongly suggest that the commercially produced pomegranate extracts should be standardized to the content of total punicalagins as well as ellagic acid. The current standard uses only ellagic acid, which is unreliable, potentially misleading and vulnerable to commercial adulteration.

Authors+Show Affiliations

Jimenez Del Rio M
Polifenoles Naturales SL, Poligono Industrial Las Majoreras Ingenio, Las Palmas, Canary Islands, Spain.
Ramazanov A
No affiliation info available
Sikorski S
No affiliation info available
Ramazanov Z
No affiliation info available
Chkhikvishvili I
No affiliation info available

MeSH

Chromatography, High Pressure LiquidEllagic AcidHealth PromotionHumansHydrolyzable TanninsIn Vitro TechniquesLythraceaePhytotherapyPlant Extracts

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17179594

Citation

Jimenez Del Rio, M, et al. "A New Method of Standartization of Health-promoting Pomegranate Fruit (Punica Granatum) Extract." Georgian Medical News, 2006, pp. 70-7.
Jimenez Del Rio M, Ramazanov A, Sikorski S, et al. A new method of standartization of health-promoting pomegranate fruit (Punica granatum) extract. Georgian Med News. 2006.
Jimenez Del Rio, M., Ramazanov, A., Sikorski, S., Ramazanov, Z., & Chkhikvishvili, I. (2006). A new method of standartization of health-promoting pomegranate fruit (Punica granatum) extract. Georgian Medical News, (140), 70-7.
Jimenez Del Rio M, et al. A New Method of Standartization of Health-promoting Pomegranate Fruit (Punica Granatum) Extract. Georgian Med News. 2006;(140)70-7. PubMed PMID: 17179594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new method of standartization of health-promoting pomegranate fruit (Punica granatum) extract. AU - Jimenez Del Rio,M, AU - Ramazanov,A, AU - Sikorski,S, AU - Ramazanov,Z, AU - Chkhikvishvili,I, PY - 2006/12/21/pubmed PY - 2007/4/11/medline PY - 2006/12/21/entrez SP - 70 EP - 7 JF - Georgian medical news JO - Georgian Med News IS - 140 N2 - This study analyzes the major phenolic constituents of pomegranate fruit juice and pericarp feedstock, and dry extracts thereof, using high-performance liquid chromatography. Pomegranate pericarp was extracted with water (WE) and alcohol (AE) as solvents, and liquid extracts were subsequently freeze-dried. The results indicate that ellagitannins punicalagin A and punicalagin B are the major constituents in the primary pomegranate feedstock and in both types of extracts. Ellagic acid, a common botanical constituent that is currently used to standardize pomegranate extracts, as well as ellagitannin punicalin, were found to be only minor constituents. Total punicalagins (the sum of punicalagins A+B and punicalin) and ellagic acid content in the pomegranate fruit pericarp feedstock WE were 7,6+/-0,3% and 0,2+/-0,1% by dry weight, respectively, and in the AE feedstock 7,0+/-0,2 and 0,4+/-0,1%, respectively. Total phenolic content (the sum of punicalagins and ellagic acid) in the pomegranate WE and AE were 45,8+/-1,2% and 42,3+/-1,1%, respectively. The concentrations of ellagic acid in the pomegranate WE and AE were 0,8+/-0,2% and 3,9+/-0,2%, respectively. Total phenolics in fresh single-strength pomegranate whole fruit juice contained 2,216+/-70 mg/L (95% punicalagins), whereas commercial pomegranate juice that was purchased from local stores was 317+/-13 mg/L (70% punicalagins). Our results strongly suggest that the commercially produced pomegranate extracts should be standardized to the content of total punicalagins as well as ellagic acid. The current standard uses only ellagic acid, which is unreliable, potentially misleading and vulnerable to commercial adulteration. SN - 1512-0112 UR - https://www.unboundmedicine.com/medline/citation/17179594/A_new_method_of_standartization_of_health_promoting_pomegranate_fruit__Punica_granatum__extract_ L2 - https://medlineplus.gov/herbalmedicine.html DB - PRIME DP - Unbound Medicine ER -