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Sugar transport systems of Bifidobacterium longum NCC2705.
J Mol Microbiol Biotechnol. 2007; 12(1-2):9-19.JM

Abstract

Here we present the complement of the carbohydrate uptake systems of the strictly anaerobic probiotic Bifidobacterium longum NCC2705. The genome analysis of this bacterium predicts that it has 19 permeases for the uptake of diverse carbohydrates. The majority belongs to the ATP-binding cassette transporter family with 13 systems identified. Among them are permeases for lactose, maltose, raffinose, and fructooligosaccharides, a commonly used prebiotic additive. We found genes that encode a complete phosphotransferase system (PTS) and genes for three permeases of the major facilitator superfamily. These systems could serve for the import of glucose, galactose, lactose, and sucrose. Growth analysis of NCC2705 cells combined with biochemical characterization and microarray data showed that the predicted substrates are consumed and that the corresponding transport and catabolic genes are expressed. Biochemical analysis of the PTS, in which proteins are central in regulation of carbon metabolism in many bacteria, revealed that B. longum has a glucose-specific PTS, while two other species (Bifidobacterium lactis and Bifidobacterium bifidum) have a fructose-6-phosphate-forming fructose-PTS instead. It became obvious that most carbohydrate systems are closely related to those from other actinomycetes, with a few exceptions. We hope that this report on B. longum carbohydrate transporter systems will serve as a guide for further in-depth analyses on the nutritional lifestyle of this beneficial bacterium.

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Authors+Show Affiliations

Parche S
Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland.
Amon J
No affiliation info available
Jankovic I
No affiliation info available
Rezzonico E
No affiliation info available
Beleut M
No affiliation info available
Barutçu H
No affiliation info available
Schendel I
No affiliation info available
Eddy MP
No affiliation info available
Burkovski A
No affiliation info available
Arigoni F
No affiliation info available
Titgemeyer F
No affiliation info available

MeSH

BifidobacteriumBiological TransportCarbohydrate MetabolismGene Expression Regulation, BacterialMembrane Transport ProteinsOligonucleotide Array Sequence AnalysisOligosaccharidesPhosphotransferasesPolysaccharides, Bacterial

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17183207

Citation

Parche, Stephan, et al. "Sugar Transport Systems of Bifidobacterium Longum NCC2705." Journal of Molecular Microbiology and Biotechnology, vol. 12, no. 1-2, 2007, pp. 9-19.
Parche S, Amon J, Jankovic I, et al. Sugar transport systems of Bifidobacterium longum NCC2705. J Mol Microbiol Biotechnol. 2007;12(1-2):9-19.
Parche, S., Amon, J., Jankovic, I., Rezzonico, E., Beleut, M., Barutçu, H., Schendel, I., Eddy, M. P., Burkovski, A., Arigoni, F., & Titgemeyer, F. (2007). Sugar transport systems of Bifidobacterium longum NCC2705. Journal of Molecular Microbiology and Biotechnology, 12(1-2), 9-19.
Parche S, et al. Sugar Transport Systems of Bifidobacterium Longum NCC2705. J Mol Microbiol Biotechnol. 2007;12(1-2):9-19. PubMed PMID: 17183207.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sugar transport systems of Bifidobacterium longum NCC2705. AU - Parche,Stephan, AU - Amon,Johannes, AU - Jankovic,Ivana, AU - Rezzonico,Enea, AU - Beleut,Manfred, AU - Barutçu,Hande, AU - Schendel,Inke, AU - Eddy,Mike P, AU - Burkovski,Andreas, AU - Arigoni,Fabrizio, AU - Titgemeyer,Fritz, PY - 2006/12/22/pubmed PY - 2007/5/3/medline PY - 2006/12/22/entrez SP - 9 EP - 19 JF - Journal of molecular microbiology and biotechnology JO - J Mol Microbiol Biotechnol VL - 12 IS - 1-2 N2 - Here we present the complement of the carbohydrate uptake systems of the strictly anaerobic probiotic Bifidobacterium longum NCC2705. The genome analysis of this bacterium predicts that it has 19 permeases for the uptake of diverse carbohydrates. The majority belongs to the ATP-binding cassette transporter family with 13 systems identified. Among them are permeases for lactose, maltose, raffinose, and fructooligosaccharides, a commonly used prebiotic additive. We found genes that encode a complete phosphotransferase system (PTS) and genes for three permeases of the major facilitator superfamily. These systems could serve for the import of glucose, galactose, lactose, and sucrose. Growth analysis of NCC2705 cells combined with biochemical characterization and microarray data showed that the predicted substrates are consumed and that the corresponding transport and catabolic genes are expressed. Biochemical analysis of the PTS, in which proteins are central in regulation of carbon metabolism in many bacteria, revealed that B. longum has a glucose-specific PTS, while two other species (Bifidobacterium lactis and Bifidobacterium bifidum) have a fructose-6-phosphate-forming fructose-PTS instead. It became obvious that most carbohydrate systems are closely related to those from other actinomycetes, with a few exceptions. We hope that this report on B. longum carbohydrate transporter systems will serve as a guide for further in-depth analyses on the nutritional lifestyle of this beneficial bacterium. SN - 1464-1801 UR - https://www.unboundmedicine.com/medline/citation/17183207/Sugar_transport_systems_of_Bifidobacterium_longum_NCC2705_ L2 - https://www.karger.com?DOI=10.1159/000096455 DB - PRIME DP - Unbound Medicine ER -