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Danggui buxue tang--a Chinese herbal decoction activates the phosphorylations of extracellular signal-regulated kinase and estrogen receptor alpha in cultured MCF-7 cells.
FEBS Lett. 2007 Jan 23; 581(2):233-40.FL

Abstract

Danggui buxue tang (DBT), a Chinese herbal decoction used to treat ailments in women, contains radix Astragali (Huangqi; RA) and radix Angelicae Sinensis (Danggui; RAS). The weight ratio of RA to RAS used in DBT must be 5:1 as stipulated as early as AD 1247; however, DBT's mechanism of action has never been described. Here, the estrogenic effects of DBT were investigated by determining the phosphorylations of estrogen receptor alpha (ER alpha) and extracellular signal-regulated kinase 1/2 (Erk1/2) in cultured MCF-7 cells. The application of DBT triggered the phosphorylation of ER alpha and Erk1/2 in a time-dependent manner. In contrast to the effect of estrogen, DBT triggered ER alpha phosphorylation at both S118 and S167. This DBT-specific phosphorylation was not triggered by an extract of one of the individual herbs, or by mixing the extracts of RA and RAS. DBT-induced downstream signals are described here. These signals suggest the uniqueness of this Chinese herbal decoction that requires a well-defined formulation.

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Authors+Show Affiliations

Gao QT
Department of Biology and Center for Chinese Medicine R&D, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, China.
Choi RC
No affiliation info available
Cheung AW
No affiliation info available
Zhu JT
No affiliation info available
Li J
No affiliation info available
Chu GK
No affiliation info available
Duan R
No affiliation info available
Cheung JK
No affiliation info available
Jiang ZY
No affiliation info available
Dong XB
No affiliation info available
Zhao KJ
No affiliation info available
Dong TT
No affiliation info available
Tsim KW
No affiliation info available

MeSH

Cells, CulturedDrugs, Chinese HerbalEstrogen Receptor alphaExtracellular Signal-Regulated MAP KinasesHumansMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3PhosphorylationPhytoestrogensPlant ExtractsTumor Cells, Cultured

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17187784

Citation

Gao, Qiu T., et al. "Danggui Buxue Tang--a Chinese Herbal Decoction Activates the Phosphorylations of Extracellular Signal-regulated Kinase and Estrogen Receptor Alpha in Cultured MCF-7 Cells." FEBS Letters, vol. 581, no. 2, 2007, pp. 233-40.
Gao QT, Choi RC, Cheung AW, et al. Danggui buxue tang--a Chinese herbal decoction activates the phosphorylations of extracellular signal-regulated kinase and estrogen receptor alpha in cultured MCF-7 cells. FEBS Lett. 2007;581(2):233-40.
Gao, Q. T., Choi, R. C., Cheung, A. W., Zhu, J. T., Li, J., Chu, G. K., Duan, R., Cheung, J. K., Jiang, Z. Y., Dong, X. B., Zhao, K. J., Dong, T. T., & Tsim, K. W. (2007). Danggui buxue tang--a Chinese herbal decoction activates the phosphorylations of extracellular signal-regulated kinase and estrogen receptor alpha in cultured MCF-7 cells. FEBS Letters, 581(2), 233-40.
Gao QT, et al. Danggui Buxue Tang--a Chinese Herbal Decoction Activates the Phosphorylations of Extracellular Signal-regulated Kinase and Estrogen Receptor Alpha in Cultured MCF-7 Cells. FEBS Lett. 2007 Jan 23;581(2):233-40. PubMed PMID: 17187784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Danggui buxue tang--a Chinese herbal decoction activates the phosphorylations of extracellular signal-regulated kinase and estrogen receptor alpha in cultured MCF-7 cells. AU - Gao,Qiu T, AU - Choi,Roy C Y, AU - Cheung,Anna W H, AU - Zhu,Judy T T, AU - Li,Jun, AU - Chu,Glanice K Y, AU - Duan,Ran, AU - Cheung,Jerry K H, AU - Jiang,Zhi Y, AU - Dong,Xiao B, AU - Zhao,Kui J, AU - Dong,Tina T X, AU - Tsim,Karl W K, Y1 - 2006/12/14/ PY - 2006/09/29/received PY - 2006/12/07/revised PY - 2006/12/07/accepted PY - 2006/12/26/pubmed PY - 2007/3/21/medline PY - 2006/12/26/entrez SP - 233 EP - 40 JF - FEBS letters JO - FEBS Lett VL - 581 IS - 2 N2 - Danggui buxue tang (DBT), a Chinese herbal decoction used to treat ailments in women, contains radix Astragali (Huangqi; RA) and radix Angelicae Sinensis (Danggui; RAS). The weight ratio of RA to RAS used in DBT must be 5:1 as stipulated as early as AD 1247; however, DBT's mechanism of action has never been described. Here, the estrogenic effects of DBT were investigated by determining the phosphorylations of estrogen receptor alpha (ER alpha) and extracellular signal-regulated kinase 1/2 (Erk1/2) in cultured MCF-7 cells. The application of DBT triggered the phosphorylation of ER alpha and Erk1/2 in a time-dependent manner. In contrast to the effect of estrogen, DBT triggered ER alpha phosphorylation at both S118 and S167. This DBT-specific phosphorylation was not triggered by an extract of one of the individual herbs, or by mixing the extracts of RA and RAS. DBT-induced downstream signals are described here. These signals suggest the uniqueness of this Chinese herbal decoction that requires a well-defined formulation. SN - 0014-5793 UR - https://www.unboundmedicine.com/medline/citation/17187784/Danggui_buxue_tang__a_Chinese_herbal_decoction_activates_the_phosphorylations_of_extracellular_signal_regulated_kinase_and_estrogen_receptor_alpha_in_cultured_MCF_7_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-5793(06)01455-4 DB - PRIME DP - Unbound Medicine ER -
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