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A novel approach for the improvement of stress resistance in wine yeasts.
Int J Food Microbiol. 2007 Feb 28; 114(1):83-91.IJ

Abstract

During wine production yeast cells are affected by several stress conditions that could affect their viability and fermentation efficiency. In this work we describe a novel genetic manipulation strategy designed to improve stress resistance in wine yeasts. This strategy involves modifying the expression of the transcription factor MSN2, which plays an important role in yeast stress responses. The promoter in one of the genomic copies of this gene has been replaced by the promoter of the SPI1 gene, encoding for a cell wall protein of unknown function. SPI1 is expressed at late phases of growth and is regulated by Msn2p. This modification allows self-induction of MSN2 expression. MSN2 gene transcription, Msn2p protein levels and cell viability increase under several stress conditions in the genetically modified strain. The expression of stress response genes regulated by Msn2p also increases under these situations. Cells containing this promoter change are able to carry out vinifications at 15 and 30 degrees C with higher fermentation rates during the first days of the process compared to the control strain.

Authors+Show Affiliations

Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València, Dr. Moliner, 50. E-46100, Burjassot, Valencia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17187885

Citation

Cardona, Fernando, et al. "A Novel Approach for the Improvement of Stress Resistance in Wine Yeasts." International Journal of Food Microbiology, vol. 114, no. 1, 2007, pp. 83-91.
Cardona F, Carrasco P, Pérez-Ortín JE, et al. A novel approach for the improvement of stress resistance in wine yeasts. Int J Food Microbiol. 2007;114(1):83-91.
Cardona, F., Carrasco, P., Pérez-Ortín, J. E., del Olmo, M. l., & Aranda, A. (2007). A novel approach for the improvement of stress resistance in wine yeasts. International Journal of Food Microbiology, 114(1), 83-91.
Cardona F, et al. A Novel Approach for the Improvement of Stress Resistance in Wine Yeasts. Int J Food Microbiol. 2007 Feb 28;114(1):83-91. PubMed PMID: 17187885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel approach for the improvement of stress resistance in wine yeasts. AU - Cardona,Fernando, AU - Carrasco,Purificación, AU - Pérez-Ortín,José Enrique, AU - del Olmo,Marcel lí, AU - Aranda,Agustín, Y1 - 2006/12/20/ PY - 2006/02/09/received PY - 2006/07/14/revised PY - 2006/10/08/accepted PY - 2006/12/26/pubmed PY - 2007/4/6/medline PY - 2006/12/26/entrez SP - 83 EP - 91 JF - International journal of food microbiology JO - Int J Food Microbiol VL - 114 IS - 1 N2 - During wine production yeast cells are affected by several stress conditions that could affect their viability and fermentation efficiency. In this work we describe a novel genetic manipulation strategy designed to improve stress resistance in wine yeasts. This strategy involves modifying the expression of the transcription factor MSN2, which plays an important role in yeast stress responses. The promoter in one of the genomic copies of this gene has been replaced by the promoter of the SPI1 gene, encoding for a cell wall protein of unknown function. SPI1 is expressed at late phases of growth and is regulated by Msn2p. This modification allows self-induction of MSN2 expression. MSN2 gene transcription, Msn2p protein levels and cell viability increase under several stress conditions in the genetically modified strain. The expression of stress response genes regulated by Msn2p also increases under these situations. Cells containing this promoter change are able to carry out vinifications at 15 and 30 degrees C with higher fermentation rates during the first days of the process compared to the control strain. SN - 0168-1605 UR - https://www.unboundmedicine.com/medline/citation/17187885/A_novel_approach_for_the_improvement_of_stress_resistance_in_wine_yeasts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1605(06)00587-3 DB - PRIME DP - Unbound Medicine ER -