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Theoretical model and experimental study of red blood cell (RBC) deformation in microchannels.
J Biomech 2007; 40(9):2088-95JB

Abstract

The motion and deformation of red blood cells (RBCs) flowing in a microchannel were studied using a theoretical model and a novel automated rheoscope. The theoretical model was developed to predict the cells deformation under shear as a function of the cells geometry and mechanical properties. Fluid dynamics and membrane mechanics are incorporated, calculating the traction and deformation in an iterative manner. The model was utilized to evaluate the effect of different biophysical parameters, such as: inner cell viscosity, membrane shear modulus and surface to volume ratio on deformation measurements. The experimental system enables the measurement of individual RBCs velocity and their deformation at defined planes within the microchannel. Good agreement was observed between the simulation results, the rheoscope measurements and published ektacytometry results. The theoretical model results imply that such deformability measuring techniques are weakly influenced by changes in the inner viscosity of the cell or the ambient fluid viscosity. However, these measurements are highly sensitive to RBC shear modulus. The shear modulus, estimated by the model and the rheoscope measurements, falls between the values obtained by micropipette aspiration and laser trapping. The study demonstrates the integration of a theoretical model with a microfabricated device in order to achieve a better understanding of RBC mechanics and their measurement using microfluidic shear assays. The system and the model have the potential of serving as quantitative clinical tools for diagnosing deformability disorders in RBCs.

Authors+Show Affiliations

Biomedical Engineering Department, Technion, Haifa 32000, Israel. korin@tx.technion.ac.ilNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17188279

Citation

Korin, Natanel, et al. "Theoretical Model and Experimental Study of Red Blood Cell (RBC) Deformation in Microchannels." Journal of Biomechanics, vol. 40, no. 9, 2007, pp. 2088-95.
Korin N, Bransky A, Dinnar U. Theoretical model and experimental study of red blood cell (RBC) deformation in microchannels. J Biomech. 2007;40(9):2088-95.
Korin, N., Bransky, A., & Dinnar, U. (2007). Theoretical model and experimental study of red blood cell (RBC) deformation in microchannels. Journal of Biomechanics, 40(9), pp. 2088-95.
Korin N, Bransky A, Dinnar U. Theoretical Model and Experimental Study of Red Blood Cell (RBC) Deformation in Microchannels. J Biomech. 2007;40(9):2088-95. PubMed PMID: 17188279.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Theoretical model and experimental study of red blood cell (RBC) deformation in microchannels. AU - Korin,Natanel, AU - Bransky,Avishay, AU - Dinnar,Uri, Y1 - 2006/12/22/ PY - 2006/07/09/received PY - 2006/10/06/accepted PY - 2006/12/26/pubmed PY - 2007/8/9/medline PY - 2006/12/26/entrez SP - 2088 EP - 95 JF - Journal of biomechanics JO - J Biomech VL - 40 IS - 9 N2 - The motion and deformation of red blood cells (RBCs) flowing in a microchannel were studied using a theoretical model and a novel automated rheoscope. The theoretical model was developed to predict the cells deformation under shear as a function of the cells geometry and mechanical properties. Fluid dynamics and membrane mechanics are incorporated, calculating the traction and deformation in an iterative manner. The model was utilized to evaluate the effect of different biophysical parameters, such as: inner cell viscosity, membrane shear modulus and surface to volume ratio on deformation measurements. The experimental system enables the measurement of individual RBCs velocity and their deformation at defined planes within the microchannel. Good agreement was observed between the simulation results, the rheoscope measurements and published ektacytometry results. The theoretical model results imply that such deformability measuring techniques are weakly influenced by changes in the inner viscosity of the cell or the ambient fluid viscosity. However, these measurements are highly sensitive to RBC shear modulus. The shear modulus, estimated by the model and the rheoscope measurements, falls between the values obtained by micropipette aspiration and laser trapping. The study demonstrates the integration of a theoretical model with a microfabricated device in order to achieve a better understanding of RBC mechanics and their measurement using microfluidic shear assays. The system and the model have the potential of serving as quantitative clinical tools for diagnosing deformability disorders in RBCs. SN - 0021-9290 UR - https://www.unboundmedicine.com/medline/citation/17188279/Theoretical_model_and_experimental_study_of_red_blood_cell__RBC__deformation_in_microchannels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9290(06)00365-4 DB - PRIME DP - Unbound Medicine ER -