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Sensitive determination of erythrosine and other red food colorants using capillary electrophoresis with laser-induced fluorescence detection.
J Chromatogr A. 2007 Feb 09; 1141(2):206-11.JC

Abstract

Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was applied to separation and sensitive determination of red food colorants. Diode pumped frequency-doubled Nd:YAG laser (532 nm) was used as an excitation source in a laboratory-built CE-LIF system. For highly fluorescent erythrosine B (E127), an extrapolated limit of detection (LOD) of 0.4 ng mL(-1) (S/N=3) was achieved. Extrapolated LODs of other tested red additives, such as carmoisine, E122 (0.5 microg mL(-1)); amaranth, E123 (0.2 microg mL(-1)); ponceau 4R, E124 (0.3 microg mL(-1)) and red 2G, E128 (0.3 microg mL(-1)) were about one-order lower compared to results obtained with CE with absorbance detection in UV/vis (CE-UV/vis). The main advantages of using CE-LIF for analysis of food samples are high selectivity and minimization of matrix effect. To our knowledge, this is the first use of CE-LIF for determination of red food colorants.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Science, Masaryk University, Kotlárská 2, 611 37 Brno, Czech Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17188280

Citation

Ryvolová, Markéta, et al. "Sensitive Determination of Erythrosine and Other Red Food Colorants Using Capillary Electrophoresis With Laser-induced Fluorescence Detection." Journal of Chromatography. A, vol. 1141, no. 2, 2007, pp. 206-11.
Ryvolová M, Táborský P, Vrábel P, et al. Sensitive determination of erythrosine and other red food colorants using capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2007;1141(2):206-11.
Ryvolová, M., Táborský, P., Vrábel, P., Krásenský, P., & Preisler, J. (2007). Sensitive determination of erythrosine and other red food colorants using capillary electrophoresis with laser-induced fluorescence detection. Journal of Chromatography. A, 1141(2), 206-11.
Ryvolová M, et al. Sensitive Determination of Erythrosine and Other Red Food Colorants Using Capillary Electrophoresis With Laser-induced Fluorescence Detection. J Chromatogr A. 2007 Feb 9;1141(2):206-11. PubMed PMID: 17188280.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitive determination of erythrosine and other red food colorants using capillary electrophoresis with laser-induced fluorescence detection. AU - Ryvolová,Markéta, AU - Táborský,Petr, AU - Vrábel,Patrik, AU - Krásenský,Pavel, AU - Preisler,Jan, Y1 - 2006/12/22/ PY - 2006/07/03/received PY - 2006/12/01/revised PY - 2006/12/05/accepted PY - 2006/12/26/pubmed PY - 2007/4/10/medline PY - 2006/12/26/entrez SP - 206 EP - 11 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1141 IS - 2 N2 - Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was applied to separation and sensitive determination of red food colorants. Diode pumped frequency-doubled Nd:YAG laser (532 nm) was used as an excitation source in a laboratory-built CE-LIF system. For highly fluorescent erythrosine B (E127), an extrapolated limit of detection (LOD) of 0.4 ng mL(-1) (S/N=3) was achieved. Extrapolated LODs of other tested red additives, such as carmoisine, E122 (0.5 microg mL(-1)); amaranth, E123 (0.2 microg mL(-1)); ponceau 4R, E124 (0.3 microg mL(-1)) and red 2G, E128 (0.3 microg mL(-1)) were about one-order lower compared to results obtained with CE with absorbance detection in UV/vis (CE-UV/vis). The main advantages of using CE-LIF for analysis of food samples are high selectivity and minimization of matrix effect. To our knowledge, this is the first use of CE-LIF for determination of red food colorants. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17188280/Sensitive_determination_of_erythrosine_and_other_red_food_colorants_using_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(06)02312-0 DB - PRIME DP - Unbound Medicine ER -