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Oxalate modulates thiobarbituric acid reactive species (TBARS) production in supernatants of homogenates from rat brain, liver and kidney: effect of diphenyl diselenide and diphenyl ditelluride.
Chem Biol Interact. 2007 Jan 30; 165(2):87-98.CB

Abstract

The aim of this paper was to investigate the mechanism(s) involved in the sodium oxalate pro-oxidative activity in vitro and the potential protection by diphenyl diselenide ((PhSe)(2)) and diphenyl ditelluride ((PhTe)(2)) using supernatants of homogenates from brain, liver and kidney. Oxalate causes a significant increase in the TBARS (thiobarbituric acid reactive species) production up to 4mmol/l and it had antioxidant activity from 8 to 16mmol/l in the brain and liver. Oxalate had no effect in kidney homogenates. The difference among tissues may be related to the formation of insoluble crystal of oxalate in kidney, but not in liver and brain homogenates. (PhSe)(2) and (PhTe)(2) reduced both basal and oxalate-induced TBARS in rat brain homogenates, whereas in liver homogenates they were antioxidant only on oxalate-induced TBARS production. (PhSe)(2) showed a modest effect on renal TBARS production, whereas (PhTe)(2) did not modulate TBARS in kidney preparations. Oxalate at 2mmol/l did not change deoxyribose degradation induced by Fe(2+) plus H(2)O(2), whereas at 20mmol/l it significantly prevents its degradation. Oxalate (up to 4mmol/l) did not alter iron (10micromol/l)-induced TBARS production in the brain preparations, whereas at 8mmol/l onwards it prevents iron effect. In liver preparations, oxalate amplifies iron pro-oxidant activity up to 4mmol/l, preventing iron-induced TBARS production at 16mmol/l onwards. These results support the antioxidant effect of organochalcogens against oxalate-induced TBARS production. In addition, our results suggest that oxalate pro- and antioxidant activity in vitro could be related to its interactions with iron ions.

Authors+Show Affiliations

Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus UFSM, Santa Maria, RS 97105-900, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17188671

Citation

Puntel, Robson Luiz, et al. "Oxalate Modulates Thiobarbituric Acid Reactive Species (TBARS) Production in Supernatants of Homogenates From Rat Brain, Liver and Kidney: Effect of Diphenyl Diselenide and Diphenyl Ditelluride." Chemico-biological Interactions, vol. 165, no. 2, 2007, pp. 87-98.
Puntel RL, Roos DH, Paixão MW, et al. Oxalate modulates thiobarbituric acid reactive species (TBARS) production in supernatants of homogenates from rat brain, liver and kidney: effect of diphenyl diselenide and diphenyl ditelluride. Chem Biol Interact. 2007;165(2):87-98.
Puntel, R. L., Roos, D. H., Paixão, M. W., Braga, A. L., Zeni, G., Nogueira, C. W., & Rocha, J. B. (2007). Oxalate modulates thiobarbituric acid reactive species (TBARS) production in supernatants of homogenates from rat brain, liver and kidney: effect of diphenyl diselenide and diphenyl ditelluride. Chemico-biological Interactions, 165(2), 87-98.
Puntel RL, et al. Oxalate Modulates Thiobarbituric Acid Reactive Species (TBARS) Production in Supernatants of Homogenates From Rat Brain, Liver and Kidney: Effect of Diphenyl Diselenide and Diphenyl Ditelluride. Chem Biol Interact. 2007 Jan 30;165(2):87-98. PubMed PMID: 17188671.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxalate modulates thiobarbituric acid reactive species (TBARS) production in supernatants of homogenates from rat brain, liver and kidney: effect of diphenyl diselenide and diphenyl ditelluride. AU - Puntel,Robson Luiz, AU - Roos,Daniel Henrique, AU - Paixão,Márcio Weber, AU - Braga,Antônio Luiz, AU - Zeni,Gilson, AU - Nogueira,Cristina Wayne, AU - Rocha,Joao Batista Teixeira, Y1 - 2006/11/23/ PY - 2006/07/31/received PY - 2006/11/03/revised PY - 2006/11/09/accepted PY - 2006/12/26/pubmed PY - 2007/3/14/medline PY - 2006/12/26/entrez SP - 87 EP - 98 JF - Chemico-biological interactions JO - Chem Biol Interact VL - 165 IS - 2 N2 - The aim of this paper was to investigate the mechanism(s) involved in the sodium oxalate pro-oxidative activity in vitro and the potential protection by diphenyl diselenide ((PhSe)(2)) and diphenyl ditelluride ((PhTe)(2)) using supernatants of homogenates from brain, liver and kidney. Oxalate causes a significant increase in the TBARS (thiobarbituric acid reactive species) production up to 4mmol/l and it had antioxidant activity from 8 to 16mmol/l in the brain and liver. Oxalate had no effect in kidney homogenates. The difference among tissues may be related to the formation of insoluble crystal of oxalate in kidney, but not in liver and brain homogenates. (PhSe)(2) and (PhTe)(2) reduced both basal and oxalate-induced TBARS in rat brain homogenates, whereas in liver homogenates they were antioxidant only on oxalate-induced TBARS production. (PhSe)(2) showed a modest effect on renal TBARS production, whereas (PhTe)(2) did not modulate TBARS in kidney preparations. Oxalate at 2mmol/l did not change deoxyribose degradation induced by Fe(2+) plus H(2)O(2), whereas at 20mmol/l it significantly prevents its degradation. Oxalate (up to 4mmol/l) did not alter iron (10micromol/l)-induced TBARS production in the brain preparations, whereas at 8mmol/l onwards it prevents iron effect. In liver preparations, oxalate amplifies iron pro-oxidant activity up to 4mmol/l, preventing iron-induced TBARS production at 16mmol/l onwards. These results support the antioxidant effect of organochalcogens against oxalate-induced TBARS production. In addition, our results suggest that oxalate pro- and antioxidant activity in vitro could be related to its interactions with iron ions. SN - 0009-2797 UR - https://www.unboundmedicine.com/medline/citation/17188671/Oxalate_modulates_thiobarbituric_acid_reactive_species__TBARS__production_in_supernatants_of_homogenates_from_rat_brain_liver_and_kidney:_effect_of_diphenyl_diselenide_and_diphenyl_ditelluride_ DB - PRIME DP - Unbound Medicine ER -