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Differential recruitment of the splicing machinery during transcription predicts genome-wide patterns of mRNA splicing.
Mol Cell. 2006 Dec 28; 24(6):903-15.MC

Abstract

The splicing machinery associates with genes to facilitate efficient cotranscriptional mRNA processing. We have mapped these associations by genome localization analysis to ascertain how splicing is achieved and regulated on a system-wide scale. Our data show that factors important for intron recognition sample nascent mRNAs and are retained specifically at intron-containing genes via RNA-dependent interactions. Spliceosome assembly proceeds cotranscriptionally but completes posttranscriptionally in most cases. Some intron-containing genes were not bound by the spliceosome, including several developmentally regulated genes. On this basis, we predicted and verified regulated splicing and observed a role for nuclear mRNA surveillance in monitoring those events. Finally, we present evidence that cotranscriptional processing events determine the recruitment of specific mRNA export factors. Broadly, our results provide mechanistic insights into the coordinated regulation of transcription, mRNA processing, and nuclear export in executing complex gene expression programs.

Links

Publisher Full Text
linkinghub.elsevier.com
linkinghub.elsevier.com

Authors+Show Affiliations

Moore MJ
Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Schwartzfarb EM
No affiliation info available
Silver PA
No affiliation info available
Yu MC
No affiliation info available

MeSH

Adenosine TriphosphatasesCell NucleusChromosome MappingGene Expression Regulation, FungalGenome, FungalIntronsModels, GeneticRNA SplicingRNA, MessengerRNA, Small NuclearRibonucleoproteinsRibonucleoproteins, Small NuclearSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSpliceosomesTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17189192

Citation

Moore, Michael J., et al. "Differential Recruitment of the Splicing Machinery During Transcription Predicts Genome-wide Patterns of mRNA Splicing." Molecular Cell, vol. 24, no. 6, 2006, pp. 903-15.
Moore MJ, Schwartzfarb EM, Silver PA, et al. Differential recruitment of the splicing machinery during transcription predicts genome-wide patterns of mRNA splicing. Mol Cell. 2006;24(6):903-15.
Moore, M. J., Schwartzfarb, E. M., Silver, P. A., & Yu, M. C. (2006). Differential recruitment of the splicing machinery during transcription predicts genome-wide patterns of mRNA splicing. Molecular Cell, 24(6), 903-15.
Moore MJ, et al. Differential Recruitment of the Splicing Machinery During Transcription Predicts Genome-wide Patterns of mRNA Splicing. Mol Cell. 2006 Dec 28;24(6):903-15. PubMed PMID: 17189192.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential recruitment of the splicing machinery during transcription predicts genome-wide patterns of mRNA splicing. AU - Moore,Michael J, AU - Schwartzfarb,Elissa M, AU - Silver,Pamela A, AU - Yu,Michael C, PY - 2006/08/18/received PY - 2006/11/30/revised PY - 2006/12/11/accepted PY - 2006/12/26/pubmed PY - 2007/1/26/medline PY - 2006/12/26/entrez SP - 903 EP - 15 JF - Molecular cell JO - Mol Cell VL - 24 IS - 6 N2 - The splicing machinery associates with genes to facilitate efficient cotranscriptional mRNA processing. We have mapped these associations by genome localization analysis to ascertain how splicing is achieved and regulated on a system-wide scale. Our data show that factors important for intron recognition sample nascent mRNAs and are retained specifically at intron-containing genes via RNA-dependent interactions. Spliceosome assembly proceeds cotranscriptionally but completes posttranscriptionally in most cases. Some intron-containing genes were not bound by the spliceosome, including several developmentally regulated genes. On this basis, we predicted and verified regulated splicing and observed a role for nuclear mRNA surveillance in monitoring those events. Finally, we present evidence that cotranscriptional processing events determine the recruitment of specific mRNA export factors. Broadly, our results provide mechanistic insights into the coordinated regulation of transcription, mRNA processing, and nuclear export in executing complex gene expression programs. SN - 1097-2765 UR - https://www.unboundmedicine.com/medline/citation/17189192/Differential_recruitment_of_the_splicing_machinery_during_transcription_predicts_genome_wide_patterns_of_mRNA_splicing_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1097-2765(06)00842-2 DB - PRIME DP - Unbound Medicine ER -