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Distribution and viral load of eight oncogenic types of human papillomavirus (HPV) and HPV 16 integration status in cervical intraepithelial neoplasia and carcinoma.
Mod Pathol 2007; 20(2):256-66MP

Abstract

Current human papillomavirus (HPV) DNA testing using pooled probes, although sensitive, lacks specificity in predicting the risk of high-grade cervical intraepithelial neoplasia (CIN 2/3) progression. To evaluate selected HPV genotyping, viral load, and viral integration status as potential predictive markers for CIN progression, we performed HPV genotyping in formalin-fixed, paraffin-embedded cervical tissue with cervical carcinoma (29 cases) and CINs (CIN 1, 27 cases; CIN 2, 28 cases; CIN 3, 33 cases). General HPVs were screened using consensus primers GP5+/GP6+ and PGMY09/11. HPV genotyping and viral load measurement were performed using quantitative real-time PCR for eight oncogenic HPV types (16, 18, 31, 33, 35, 45, 52, and 58). HPV 16 viral integration status was evaluated by measuring HPV 16 E2/E6 ratio. We observed that HPV DNA positivity increased in parallel with the severity of CINs and carcinoma, with 59% positivity in CIN 1, 68% in CIN 2, 76% in CIN 3, and 97% in carcinoma (P trend=0.004). The eight oncogenic HPV types were significantly associated with CIN 2/3 (81%) and carcinoma (93%) (odds ratio (OR), 15.0; 95% confidence interval (CI), 5.67-39.76; P<0.0001) compared with the unknown HPV types (OR, 2.87; 95% CI, 0.89-9.22; P=0.08). HPV 16 was the predominant oncogenic HPV type in CIN 2/3 (51%) and carcinoma (71%) and integrated significantly more frequently in carcinoma than in CIN 2/3 (P=0.004). No significant differences in viral load were observed across the disease categories. Our findings suggest that selected genotyping for the eight oncogenic HPV types might be useful in separating women with a higher risk of CIN progression from those with a minimal risk. We also conclude that the HPV 16 integration status has potential to be a marker for risk assessment of CIN progression.

Authors+Show Affiliations

Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030-4009, USA. mguo@mdanderson.orgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17192787

Citation

Guo, Ming, et al. "Distribution and Viral Load of Eight Oncogenic Types of Human Papillomavirus (HPV) and HPV 16 Integration Status in Cervical Intraepithelial Neoplasia and Carcinoma." Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc, vol. 20, no. 2, 2007, pp. 256-66.
Guo M, Sneige N, Silva EG, et al. Distribution and viral load of eight oncogenic types of human papillomavirus (HPV) and HPV 16 integration status in cervical intraepithelial neoplasia and carcinoma. Mod Pathol. 2007;20(2):256-66.
Guo, M., Sneige, N., Silva, E. G., Jan, Y. J., Cogdell, D. E., Lin, E., ... Zhang, W. (2007). Distribution and viral load of eight oncogenic types of human papillomavirus (HPV) and HPV 16 integration status in cervical intraepithelial neoplasia and carcinoma. Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc, 20(2), pp. 256-66.
Guo M, et al. Distribution and Viral Load of Eight Oncogenic Types of Human Papillomavirus (HPV) and HPV 16 Integration Status in Cervical Intraepithelial Neoplasia and Carcinoma. Mod Pathol. 2007;20(2):256-66. PubMed PMID: 17192787.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution and viral load of eight oncogenic types of human papillomavirus (HPV) and HPV 16 integration status in cervical intraepithelial neoplasia and carcinoma. AU - Guo,Ming, AU - Sneige,Nour, AU - Silva,Elvio G, AU - Jan,Yee Jee, AU - Cogdell,David E, AU - Lin,E, AU - Luthra,Rajyalakshmi, AU - Zhang,Wei, Y1 - 2006/12/22/ PY - 2006/12/29/pubmed PY - 2007/4/20/medline PY - 2006/12/29/entrez SP - 256 EP - 66 JF - Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc JO - Mod. Pathol. VL - 20 IS - 2 N2 - Current human papillomavirus (HPV) DNA testing using pooled probes, although sensitive, lacks specificity in predicting the risk of high-grade cervical intraepithelial neoplasia (CIN 2/3) progression. To evaluate selected HPV genotyping, viral load, and viral integration status as potential predictive markers for CIN progression, we performed HPV genotyping in formalin-fixed, paraffin-embedded cervical tissue with cervical carcinoma (29 cases) and CINs (CIN 1, 27 cases; CIN 2, 28 cases; CIN 3, 33 cases). General HPVs were screened using consensus primers GP5+/GP6+ and PGMY09/11. HPV genotyping and viral load measurement were performed using quantitative real-time PCR for eight oncogenic HPV types (16, 18, 31, 33, 35, 45, 52, and 58). HPV 16 viral integration status was evaluated by measuring HPV 16 E2/E6 ratio. We observed that HPV DNA positivity increased in parallel with the severity of CINs and carcinoma, with 59% positivity in CIN 1, 68% in CIN 2, 76% in CIN 3, and 97% in carcinoma (P trend=0.004). The eight oncogenic HPV types were significantly associated with CIN 2/3 (81%) and carcinoma (93%) (odds ratio (OR), 15.0; 95% confidence interval (CI), 5.67-39.76; P<0.0001) compared with the unknown HPV types (OR, 2.87; 95% CI, 0.89-9.22; P=0.08). HPV 16 was the predominant oncogenic HPV type in CIN 2/3 (51%) and carcinoma (71%) and integrated significantly more frequently in carcinoma than in CIN 2/3 (P=0.004). No significant differences in viral load were observed across the disease categories. Our findings suggest that selected genotyping for the eight oncogenic HPV types might be useful in separating women with a higher risk of CIN progression from those with a minimal risk. We also conclude that the HPV 16 integration status has potential to be a marker for risk assessment of CIN progression. SN - 0893-3952 UR - https://www.unboundmedicine.com/medline/citation/17192787/Distribution_and_viral_load_of_eight_oncogenic_types_of_human_papillomavirus__HPV__and_HPV_16_integration_status_in_cervical_intraepithelial_neoplasia_and_carcinoma_ L2 - http://dx.doi.org/10.1038/modpathol.3800737 DB - PRIME DP - Unbound Medicine ER -