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Immunosuppressive activity of the ethanol extract of Siegesbeckia orientalis on the immune responses to ovalbumin in mice.
Chem Biodivers. 2006 Jul; 3(7):754-61.CB

Abstract

The in vitro and in vivo immunosuppressive activity of the ethanol extract of Siegesbeckia orientalis (EESO) was studied on the immune responses in mice. EESO significantly suppressed concanavalin A (Con A)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. ICR Mice were immunized subcutaneously with ovalbumin (OVA) on days 0 and 14. Beginning on the day of immunization, the mice were administered intraperitoneally with EESO at a single dose of 0.25, 0.5, and 1.0 mg at intervals of 7 days. On day 28, OVA-specific antibodies in serum, and mitogen- and OVA-induced splenocyte proliferation were measured. EESO significantly suppressed Con A-, LPS- and OVA-induced splenocyte proliferation in the OVA-immunized mice in a dose-dependent manner. The OVA-specific serum IgG, IgG1, and IgG2b levels in the OVA-immunized mice were also significantly reduced by EESO. Moreover, reducing effect on the IgG1 antibody of EESO at the dose of 1.0 mg was more significant than that of cyclosporin A (CsA; positive drug). The results suggest that EESO could suppress the cellular and humoral response to ovalbumin in mice, and deserve further investigations to be developed as immunosuppressant.

Authors+Show Affiliations

College of Animal Science, Zhejiang University, Hangzhou 310029, PR China. sunhx@zju.edu.cnNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17193307

Citation

Sun, Hong-Xiang, and Hua Wang. "Immunosuppressive Activity of the Ethanol Extract of Siegesbeckia Orientalis On the Immune Responses to Ovalbumin in Mice." Chemistry & Biodiversity, vol. 3, no. 7, 2006, pp. 754-61.
Sun HX, Wang H. Immunosuppressive activity of the ethanol extract of Siegesbeckia orientalis on the immune responses to ovalbumin in mice. Chem Biodivers. 2006;3(7):754-61.
Sun, H. X., & Wang, H. (2006). Immunosuppressive activity of the ethanol extract of Siegesbeckia orientalis on the immune responses to ovalbumin in mice. Chemistry & Biodiversity, 3(7), 754-61.
Sun HX, Wang H. Immunosuppressive Activity of the Ethanol Extract of Siegesbeckia Orientalis On the Immune Responses to Ovalbumin in Mice. Chem Biodivers. 2006;3(7):754-61. PubMed PMID: 17193307.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunosuppressive activity of the ethanol extract of Siegesbeckia orientalis on the immune responses to ovalbumin in mice. AU - Sun,Hong-Xiang, AU - Wang,Hua, PY - 2006/12/29/pubmed PY - 2007/3/23/medline PY - 2006/12/29/entrez SP - 754 EP - 61 JF - Chemistry & biodiversity JO - Chem Biodivers VL - 3 IS - 7 N2 - The in vitro and in vivo immunosuppressive activity of the ethanol extract of Siegesbeckia orientalis (EESO) was studied on the immune responses in mice. EESO significantly suppressed concanavalin A (Con A)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. ICR Mice were immunized subcutaneously with ovalbumin (OVA) on days 0 and 14. Beginning on the day of immunization, the mice were administered intraperitoneally with EESO at a single dose of 0.25, 0.5, and 1.0 mg at intervals of 7 days. On day 28, OVA-specific antibodies in serum, and mitogen- and OVA-induced splenocyte proliferation were measured. EESO significantly suppressed Con A-, LPS- and OVA-induced splenocyte proliferation in the OVA-immunized mice in a dose-dependent manner. The OVA-specific serum IgG, IgG1, and IgG2b levels in the OVA-immunized mice were also significantly reduced by EESO. Moreover, reducing effect on the IgG1 antibody of EESO at the dose of 1.0 mg was more significant than that of cyclosporin A (CsA; positive drug). The results suggest that EESO could suppress the cellular and humoral response to ovalbumin in mice, and deserve further investigations to be developed as immunosuppressant. SN - 1612-1880 UR - https://www.unboundmedicine.com/medline/citation/17193307/Immunosuppressive_activity_of_the_ethanol_extract_of_Siegesbeckia_orientalis_on_the_immune_responses_to_ovalbumin_in_mice_ L2 - https://doi.org/10.1002/cbdv.200690077 DB - PRIME DP - Unbound Medicine ER -