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Immunosuppressive activity of Semen Persicae ethanol extract on specific antibody and cellular response to ovalbumin in mice.
Chem Biodivers. 2006 Sep; 3(9):967-74.CB

Abstract

The immunosuppressive activity of the ethanol extract of Semen Persicae (EESP) was studied with respect to specific antibody and cellular response to ovalbumin (OVA) in mice. The effects of EESP on mice splenocyte proliferation in vitro were measured. EESP significantly suppressed concanavalin A (ConA)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. Furthermore, the effects of EESP at three dose levels on the humoral and cellular immune responses in the OVA-immunized mice were examined. ICR Mice were immunized subcutaneously with OVA on day 0 and 14. Starting on the day of immunization, the mice were administered intraperitoneally with EESP at a single dose of 0.25, 0.5, and 1.0 mg, and cyclosporin A (CsA, positive drug) at a single dose of 0.1 mg at intervals of 7 days. On day 28, mitogen- and OVA-induced splenocyte proliferation and OVA-specific antibody level in serum were measured. EESP significantly decreased ConA-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice at the dose of 1.0 mg. Meanwhile, the OVA-specific serum IgG, IgG1, and IgG2b antibody levels in the OVA-immunized mice were markedly reduced by EESP in a dose-dependent manner. The results suggest that EESP could suppress the cellular and humoral immune response in mice, and deserve further research to be developed as immunosuppressant.

Authors+Show Affiliations

College of Animal Sciences, Zhejiang University, Hangzhou 310029, P.R. China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17193328

Citation

Zhang, Yi-Bin, et al. "Immunosuppressive Activity of Semen Persicae Ethanol Extract On Specific Antibody and Cellular Response to Ovalbumin in Mice." Chemistry & Biodiversity, vol. 3, no. 9, 2006, pp. 967-74.
Zhang YB, Qin F, Sun HX. Immunosuppressive activity of Semen Persicae ethanol extract on specific antibody and cellular response to ovalbumin in mice. Chem Biodivers. 2006;3(9):967-74.
Zhang, Y. B., Qin, F., & Sun, H. X. (2006). Immunosuppressive activity of Semen Persicae ethanol extract on specific antibody and cellular response to ovalbumin in mice. Chemistry & Biodiversity, 3(9), 967-74.
Zhang YB, Qin F, Sun HX. Immunosuppressive Activity of Semen Persicae Ethanol Extract On Specific Antibody and Cellular Response to Ovalbumin in Mice. Chem Biodivers. 2006;3(9):967-74. PubMed PMID: 17193328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunosuppressive activity of Semen Persicae ethanol extract on specific antibody and cellular response to ovalbumin in mice. AU - Zhang,Yi-Bin, AU - Qin,Feng, AU - Sun,Hong-Xiang, PY - 2006/12/29/pubmed PY - 2007/3/16/medline PY - 2006/12/29/entrez SP - 967 EP - 74 JF - Chemistry & biodiversity JO - Chem Biodivers VL - 3 IS - 9 N2 - The immunosuppressive activity of the ethanol extract of Semen Persicae (EESP) was studied with respect to specific antibody and cellular response to ovalbumin (OVA) in mice. The effects of EESP on mice splenocyte proliferation in vitro were measured. EESP significantly suppressed concanavalin A (ConA)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. Furthermore, the effects of EESP at three dose levels on the humoral and cellular immune responses in the OVA-immunized mice were examined. ICR Mice were immunized subcutaneously with OVA on day 0 and 14. Starting on the day of immunization, the mice were administered intraperitoneally with EESP at a single dose of 0.25, 0.5, and 1.0 mg, and cyclosporin A (CsA, positive drug) at a single dose of 0.1 mg at intervals of 7 days. On day 28, mitogen- and OVA-induced splenocyte proliferation and OVA-specific antibody level in serum were measured. EESP significantly decreased ConA-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice at the dose of 1.0 mg. Meanwhile, the OVA-specific serum IgG, IgG1, and IgG2b antibody levels in the OVA-immunized mice were markedly reduced by EESP in a dose-dependent manner. The results suggest that EESP could suppress the cellular and humoral immune response in mice, and deserve further research to be developed as immunosuppressant. SN - 1612-1880 UR - https://www.unboundmedicine.com/medline/citation/17193328/Immunosuppressive_activity_of_Semen_Persicae_ethanol_extract_on_specific_antibody_and_cellular_response_to_ovalbumin_in_mice_ L2 - https://doi.org/10.1002/cbdv.200690105 DB - PRIME DP - Unbound Medicine ER -