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Biophysical and biochemical properties of CR326 human hepatitis A virus.

Abstract

CR326 human hepatitis A virus purified by isopycnic banding from infected marmoset sera was shown to consist of 27 mmu spherical particles on electron microscopic examination. The particles were identified as hepatitis A virus by tests by infectivity and by specific neutralization of infectivity with convalescent human hepatitis A serum. Also, identical 27 mmu viruses in liver extracts gave specific reactions with hepatitis A antisera when tested by immune electron microscopy. The buoyant density of the virus in CsCl was 1.34 and it was heat (60 C), ether, and acid stable but was destroyed by heat (100 C), formalin (1:4000), and ultraviolet irradiation. Electron microscopic studies of sections of infected marmoset liver showed intracytoplasmic virus particles, usually in vesicles. Presumptive findings for RNA, together with the intracytoplasmic location of the virus, indicated the virus to be of RNA-type. The attributes of the virus indicate it is closely related to the enterovirus family and not to hepatitis B virus.

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    MeSH

    Animals
    Antigen-Antibody Reactions
    Callitrichinae
    Hepatitis A
    Hepatitis B Antigens
    Hepatitis B virus
    Hepatitis Viruses
    Hepatovirus
    Hot Temperature
    Humans
    Immune Adherence Reaction
    Inclusion Bodies, Viral
    Liver
    Pan troglodytes
    RNA, Viral

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    171955

    Citation

    Provost, P J., et al. "Biophysical and Biochemical Properties of CR326 Human Hepatitis a Virus." The American Journal of the Medical Sciences, vol. 270, no. 1, 1975, pp. 87-92.
    Provost PJ, Wolanski BS, Miller WJ, et al. Biophysical and biochemical properties of CR326 human hepatitis A virus. Am J Med Sci. 1975;270(1):87-92.
    Provost, P. J., Wolanski, B. S., Miller, W. J., Ittensohn, O. L., McAleer, W. J., & Hilleman, M. R. (1975). Biophysical and biochemical properties of CR326 human hepatitis A virus. The American Journal of the Medical Sciences, 270(1), pp. 87-92.
    Provost PJ, et al. Biophysical and Biochemical Properties of CR326 Human Hepatitis a Virus. Am J Med Sci. 1975;270(1):87-92. PubMed PMID: 171955.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Biophysical and biochemical properties of CR326 human hepatitis A virus. AU - Provost,P J, AU - Wolanski,B S, AU - Miller,W J, AU - Ittensohn,O L, AU - McAleer,W J, AU - Hilleman,M R, PY - 1975/7/1/pubmed PY - 1975/7/1/medline PY - 1975/7/1/entrez SP - 87 EP - 92 JF - The American journal of the medical sciences JO - Am. J. Med. Sci. VL - 270 IS - 1 N2 - CR326 human hepatitis A virus purified by isopycnic banding from infected marmoset sera was shown to consist of 27 mmu spherical particles on electron microscopic examination. The particles were identified as hepatitis A virus by tests by infectivity and by specific neutralization of infectivity with convalescent human hepatitis A serum. Also, identical 27 mmu viruses in liver extracts gave specific reactions with hepatitis A antisera when tested by immune electron microscopy. The buoyant density of the virus in CsCl was 1.34 and it was heat (60 C), ether, and acid stable but was destroyed by heat (100 C), formalin (1:4000), and ultraviolet irradiation. Electron microscopic studies of sections of infected marmoset liver showed intracytoplasmic virus particles, usually in vesicles. Presumptive findings for RNA, together with the intracytoplasmic location of the virus, indicated the virus to be of RNA-type. The attributes of the virus indicate it is closely related to the enterovirus family and not to hepatitis B virus. SN - 0002-9629 UR - https://www.unboundmedicine.com/medline/citation/171955/Biophysical_and_biochemical_properties_of_CR326_human_hepatitis_A_virus_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=171955.ui DB - PRIME DP - Unbound Medicine ER -