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Interferons and the colony-stimulating factors IL-3 and GM-CSF enhance the IFN-alpha response in human blood leucocytes induced by herpes simplex virus.
Scand J Immunol 1991; 34(5):549-55SJ

Abstract

Human peripheral blood mononuclear cells (PBMC) were stimulated to produce interferon-alpha (IFN-alpha) by glutaraldehyde-fixed Herpes simplex virus type 1 (HSV)-infected WISH amnion cells in vitro. Different cytokines were included during the stimulation and tested for their ability to enhance the IFN-alpha response which occurs in the natural IFN-alpha producing (NIP) leucocytes. The total production of IFN-alpha and the numbers of IFN-alpha producing cells (IPCs) were increased by interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF). Their most marked effect was to reduce the time required for induction of the IPC by the HSV-infected cells, thereby causing both an earlier peak of IPC numbers and secretion of IFN-alpha. Addition of IFN-alpha 2b did not alter the kinetics of the IFN-alpha response in the same way as the two CSFs, but instead generally increased the IPC numbers and the production of IFN-alpha. The IL-3 and GM-CSF, especially in combination with IFN-alpha, had the most pronounced enhancing effects on IPC numbers when PBMC were induced at low cell concentrations. The cytokines IL-1, IL-2, IL-4, IL-6 or tumour necrosis factor-alpha (TNF-alpha) had no detectable effects on the IFN-alpha response. The results suggest that cytokines such as the CSFs and IFNs may be involved in the regulation of NIP cell functions.

Authors+Show Affiliations

Interferon Laboratory, Uppsala University, Sweden.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1719612

Citation

Cederblad, B, and G V. Alm. "Interferons and the Colony-stimulating Factors IL-3 and GM-CSF Enhance the IFN-alpha Response in Human Blood Leucocytes Induced By Herpes Simplex Virus." Scandinavian Journal of Immunology, vol. 34, no. 5, 1991, pp. 549-55.
Cederblad B, Alm GV. Interferons and the colony-stimulating factors IL-3 and GM-CSF enhance the IFN-alpha response in human blood leucocytes induced by herpes simplex virus. Scand J Immunol. 1991;34(5):549-55.
Cederblad, B., & Alm, G. V. (1991). Interferons and the colony-stimulating factors IL-3 and GM-CSF enhance the IFN-alpha response in human blood leucocytes induced by herpes simplex virus. Scandinavian Journal of Immunology, 34(5), pp. 549-55.
Cederblad B, Alm GV. Interferons and the Colony-stimulating Factors IL-3 and GM-CSF Enhance the IFN-alpha Response in Human Blood Leucocytes Induced By Herpes Simplex Virus. Scand J Immunol. 1991;34(5):549-55. PubMed PMID: 1719612.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interferons and the colony-stimulating factors IL-3 and GM-CSF enhance the IFN-alpha response in human blood leucocytes induced by herpes simplex virus. AU - Cederblad,B, AU - Alm,G V, PY - 1991/11/1/pubmed PY - 1991/11/1/medline PY - 1991/11/1/entrez SP - 549 EP - 55 JF - Scandinavian journal of immunology JO - Scand. J. Immunol. VL - 34 IS - 5 N2 - Human peripheral blood mononuclear cells (PBMC) were stimulated to produce interferon-alpha (IFN-alpha) by glutaraldehyde-fixed Herpes simplex virus type 1 (HSV)-infected WISH amnion cells in vitro. Different cytokines were included during the stimulation and tested for their ability to enhance the IFN-alpha response which occurs in the natural IFN-alpha producing (NIP) leucocytes. The total production of IFN-alpha and the numbers of IFN-alpha producing cells (IPCs) were increased by interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF). Their most marked effect was to reduce the time required for induction of the IPC by the HSV-infected cells, thereby causing both an earlier peak of IPC numbers and secretion of IFN-alpha. Addition of IFN-alpha 2b did not alter the kinetics of the IFN-alpha response in the same way as the two CSFs, but instead generally increased the IPC numbers and the production of IFN-alpha. The IL-3 and GM-CSF, especially in combination with IFN-alpha, had the most pronounced enhancing effects on IPC numbers when PBMC were induced at low cell concentrations. The cytokines IL-1, IL-2, IL-4, IL-6 or tumour necrosis factor-alpha (TNF-alpha) had no detectable effects on the IFN-alpha response. The results suggest that cytokines such as the CSFs and IFNs may be involved in the regulation of NIP cell functions. SN - 0300-9475 UR - https://www.unboundmedicine.com/medline/citation/1719612/Interferons_and_the_colony_stimulating_factors_IL_3_and_GM_CSF_enhance_the_IFN_alpha_response_in_human_blood_leucocytes_induced_by_herpes_simplex_virus_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0300-9475&date=1991&volume=34&issue=5&spage=549 DB - PRIME DP - Unbound Medicine ER -