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Assessment of physical and antioxidant activity stability, in vitro release and in vivo efficacy of formulations added with superoxide dismutase alone or in association with alpha-tocopherol.
Eur J Pharm Biopharm. 2007 Jun; 66(3):451-9.EJ

Abstract

A topical formulation was added with different concentrations of superoxide dismutase (SOD) alone or in association with alpha-tocopherol (alpha-TOC). The physical stability was evaluated by rheological behavior of formulations stored at 4 degrees C, 30 degrees C/60% RH and 40 degrees C/70% RH for 6 months. SOD alone and formulations containing SOD 0.2%, 0.4% or 0.6% or SOD and alpha-TOC were stored in the same conditions and the enzymatic activity was evaluated by the superoxide anion scavenging using chemiluminescence measurement. In vitro release study was carried out using modified Franz diffusion cell and SOD formulations photoprotection against skin erythema was observed for 72 h. SOD and alpha-TOC formulation proved to be instable, since the interaction between the antioxidants led to both physical and enzymatic activity instability. SOD formulations showed to be physically stable and maintained the enzymatic activity for 6 months when stored at 4 and 30 degrees C/60% RH. Despite the fact of low SOD release from the formulation, it was effective in inhibiting the UVB-induced skin erythema for 48 h after a single application. Topical administration of antioxidants provides an efficient way to enrich the endogenous cutaneous protection system, and SOD formulations could be used for improving photoprotection of skin.

Authors+Show Affiliations

Department of Pharmaceutical Science, University of São Paolo, Ribeirão Preto, Brazil. valeriadimambro@yahoo.com.brNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17196809

Citation

Di Mambro, Valéria Maria, and Maria José Vieira Fonseca. "Assessment of Physical and Antioxidant Activity Stability, in Vitro Release and in Vivo Efficacy of Formulations Added With Superoxide Dismutase Alone or in Association With Alpha-tocopherol." European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, vol. 66, no. 3, 2007, pp. 451-9.
Di Mambro VM, Fonseca MJ. Assessment of physical and antioxidant activity stability, in vitro release and in vivo efficacy of formulations added with superoxide dismutase alone or in association with alpha-tocopherol. Eur J Pharm Biopharm. 2007;66(3):451-9.
Di Mambro, V. M., & Fonseca, M. J. (2007). Assessment of physical and antioxidant activity stability, in vitro release and in vivo efficacy of formulations added with superoxide dismutase alone or in association with alpha-tocopherol. European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, 66(3), 451-9.
Di Mambro VM, Fonseca MJ. Assessment of Physical and Antioxidant Activity Stability, in Vitro Release and in Vivo Efficacy of Formulations Added With Superoxide Dismutase Alone or in Association With Alpha-tocopherol. Eur J Pharm Biopharm. 2007;66(3):451-9. PubMed PMID: 17196809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of physical and antioxidant activity stability, in vitro release and in vivo efficacy of formulations added with superoxide dismutase alone or in association with alpha-tocopherol. AU - Di Mambro,Valéria Maria, AU - Fonseca,Maria José Vieira, Y1 - 2006/11/26/ PY - 2005/08/25/received PY - 2006/11/16/revised PY - 2006/11/16/accepted PY - 2007/1/2/pubmed PY - 2007/8/8/medline PY - 2007/1/2/entrez SP - 451 EP - 9 JF - European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V JO - Eur J Pharm Biopharm VL - 66 IS - 3 N2 - A topical formulation was added with different concentrations of superoxide dismutase (SOD) alone or in association with alpha-tocopherol (alpha-TOC). The physical stability was evaluated by rheological behavior of formulations stored at 4 degrees C, 30 degrees C/60% RH and 40 degrees C/70% RH for 6 months. SOD alone and formulations containing SOD 0.2%, 0.4% or 0.6% or SOD and alpha-TOC were stored in the same conditions and the enzymatic activity was evaluated by the superoxide anion scavenging using chemiluminescence measurement. In vitro release study was carried out using modified Franz diffusion cell and SOD formulations photoprotection against skin erythema was observed for 72 h. SOD and alpha-TOC formulation proved to be instable, since the interaction between the antioxidants led to both physical and enzymatic activity instability. SOD formulations showed to be physically stable and maintained the enzymatic activity for 6 months when stored at 4 and 30 degrees C/60% RH. Despite the fact of low SOD release from the formulation, it was effective in inhibiting the UVB-induced skin erythema for 48 h after a single application. Topical administration of antioxidants provides an efficient way to enrich the endogenous cutaneous protection system, and SOD formulations could be used for improving photoprotection of skin. SN - 0939-6411 UR - https://www.unboundmedicine.com/medline/citation/17196809/Assessment_of_physical_and_antioxidant_activity_stability_in_vitro_release_and_in_vivo_efficacy_of_formulations_added_with_superoxide_dismutase_alone_or_in_association_with_alpha_tocopherol_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0939-6411(06)00334-1 DB - PRIME DP - Unbound Medicine ER -