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Comparison of proanthocyanidins in commercial antioxidants: grape seed and pine bark extracts.
J Agric Food Chem. 2007 Jan 10; 55(1):148-56.JA

Abstract

The major constituents in grape seed and pine bark extracts are proanthocyanidins. To evaluate material available to consumers, select lots were analyzed using high-performance liquid chromatography, gas chromatography/mass spectrometry (GC/MS), liquid chromatography/mass spectrometry (LC/MS), gel permeation chromatography (GPC), and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Atmospheric pressure chemical ionization (APCI) LC/MS was used to identify monomers, dimers, and trimers present. GC/MS analyses led to the identification of ethyl esters of hexadecanoic acid, linoleic acid, and oleic acid, as well as smaller phenolic and terpene components. The GPC molecular weight (MW) distribution indicated components ranging from approximately 162 to approximately 5500 MW (pine bark less than 1180 MW and grape seed approximately 1180 to approximately 5000 MW). MALDI-TOF MS analyses showed that pine bark did not contain oligomers with odd numbers of gallate units and grape seed contained oligomers with both odd and even numbers of gallate. Reflectron MALDI-TOF MS identified oligomers up to a pentamer and heptamer, and linear MALDI-TOF MS showed a mass range nearly double that of reflectron analyses.

Authors+Show Affiliations

Midwest Research Institute, 425 Volker Boulevard, Kansas City, Missouri 64110, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17199326

Citation

Weber, Holly A., et al. "Comparison of Proanthocyanidins in Commercial Antioxidants: Grape Seed and Pine Bark Extracts." Journal of Agricultural and Food Chemistry, vol. 55, no. 1, 2007, pp. 148-56.
Weber HA, Hodges AE, Guthrie JR, et al. Comparison of proanthocyanidins in commercial antioxidants: grape seed and pine bark extracts. J Agric Food Chem. 2007;55(1):148-56.
Weber, H. A., Hodges, A. E., Guthrie, J. R., O'Brien, B. M., Robaugh, D., Clark, A. P., Harris, R. K., Algaier, J. W., & Smith, C. S. (2007). Comparison of proanthocyanidins in commercial antioxidants: grape seed and pine bark extracts. Journal of Agricultural and Food Chemistry, 55(1), 148-56.
Weber HA, et al. Comparison of Proanthocyanidins in Commercial Antioxidants: Grape Seed and Pine Bark Extracts. J Agric Food Chem. 2007 Jan 10;55(1):148-56. PubMed PMID: 17199326.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of proanthocyanidins in commercial antioxidants: grape seed and pine bark extracts. AU - Weber,Holly A, AU - Hodges,Andrew E, AU - Guthrie,Jill R, AU - O'Brien,Brandon M, AU - Robaugh,David, AU - Clark,Alice P, AU - Harris,Roger K, AU - Algaier,Joseph W, AU - Smith,Cynthia S, PY - 2007/1/4/pubmed PY - 2007/3/29/medline PY - 2007/1/4/entrez SP - 148 EP - 56 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 55 IS - 1 N2 - The major constituents in grape seed and pine bark extracts are proanthocyanidins. To evaluate material available to consumers, select lots were analyzed using high-performance liquid chromatography, gas chromatography/mass spectrometry (GC/MS), liquid chromatography/mass spectrometry (LC/MS), gel permeation chromatography (GPC), and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Atmospheric pressure chemical ionization (APCI) LC/MS was used to identify monomers, dimers, and trimers present. GC/MS analyses led to the identification of ethyl esters of hexadecanoic acid, linoleic acid, and oleic acid, as well as smaller phenolic and terpene components. The GPC molecular weight (MW) distribution indicated components ranging from approximately 162 to approximately 5500 MW (pine bark less than 1180 MW and grape seed approximately 1180 to approximately 5000 MW). MALDI-TOF MS analyses showed that pine bark did not contain oligomers with odd numbers of gallate units and grape seed contained oligomers with both odd and even numbers of gallate. Reflectron MALDI-TOF MS identified oligomers up to a pentamer and heptamer, and linear MALDI-TOF MS showed a mass range nearly double that of reflectron analyses. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/17199326/Comparison_of_proanthocyanidins_in_commercial_antioxidants:_grape_seed_and_pine_bark_extracts_ DB - PRIME DP - Unbound Medicine ER -