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Isolation and structure elucidation of procyanidin oligomers from Saskatoon berries (Amelanchier alnifolia).
J Agric Food Chem. 2007 Jan 10; 55(1):157-64.JA

Abstract

Proanthocyanidin oligomers with different degrees of polymerization were isolated from Saskatoon berries (Amelanchier alnifolia) by means of gel adsorption and normal-phase liquid chromatography. The proanthocyanidins were identified using electrospray ionization mass spectrometry, nuclear magnetic resonance spectroscopy, and thiolytic degradation coupled with reversed-phase liquid chromatography. The results established that Saskatoon berries contain proanthocyanidins from dimers through heptamers and higher polymers. Saskatoon proanthocyanidins are essentially of procyanidin type, consisting mainly of epicatechin units linked by B-type bonds. The simple procyanidin profile of Saskatoon berries allowed the procyanidins to be separated precisely according to their degrees of polymerization. In the future they can be used as standard compounds for qualitative and quantitative analysis of procyanidins as well as for elucidation of the biological activities of proanthocyanidins.

Authors+Show Affiliations

MTT Agrifood Research Finland, Biotechnology and Food Research, Myllytie 1, FI-31600, Jokioinen, Finland. jarkko.hellstrom@mtt.fiNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17199327

Citation

Hellström, Jarkko, et al. "Isolation and Structure Elucidation of Procyanidin Oligomers From Saskatoon Berries (Amelanchier Alnifolia)." Journal of Agricultural and Food Chemistry, vol. 55, no. 1, 2007, pp. 157-64.
Hellström J, Sinkkonen J, Karonen M, et al. Isolation and structure elucidation of procyanidin oligomers from Saskatoon berries (Amelanchier alnifolia). J Agric Food Chem. 2007;55(1):157-64.
Hellström, J., Sinkkonen, J., Karonen, M., & Mattila, P. (2007). Isolation and structure elucidation of procyanidin oligomers from Saskatoon berries (Amelanchier alnifolia). Journal of Agricultural and Food Chemistry, 55(1), 157-64.
Hellström J, et al. Isolation and Structure Elucidation of Procyanidin Oligomers From Saskatoon Berries (Amelanchier Alnifolia). J Agric Food Chem. 2007 Jan 10;55(1):157-64. PubMed PMID: 17199327.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and structure elucidation of procyanidin oligomers from Saskatoon berries (Amelanchier alnifolia). AU - Hellström,Jarkko, AU - Sinkkonen,Jari, AU - Karonen,Maarit, AU - Mattila,Pirjo, PY - 2007/1/4/pubmed PY - 2007/3/29/medline PY - 2007/1/4/entrez SP - 157 EP - 64 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 55 IS - 1 N2 - Proanthocyanidin oligomers with different degrees of polymerization were isolated from Saskatoon berries (Amelanchier alnifolia) by means of gel adsorption and normal-phase liquid chromatography. The proanthocyanidins were identified using electrospray ionization mass spectrometry, nuclear magnetic resonance spectroscopy, and thiolytic degradation coupled with reversed-phase liquid chromatography. The results established that Saskatoon berries contain proanthocyanidins from dimers through heptamers and higher polymers. Saskatoon proanthocyanidins are essentially of procyanidin type, consisting mainly of epicatechin units linked by B-type bonds. The simple procyanidin profile of Saskatoon berries allowed the procyanidins to be separated precisely according to their degrees of polymerization. In the future they can be used as standard compounds for qualitative and quantitative analysis of procyanidins as well as for elucidation of the biological activities of proanthocyanidins. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/17199327/Isolation_and_structure_elucidation_of_procyanidin_oligomers_from_Saskatoon_berries__Amelanchier_alnifolia__ L2 - https://dx.doi.org/10.1021/jf062441t DB - PRIME DP - Unbound Medicine ER -