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Growth suppression of human cancer cells by polyphenolics from sweetpotato (Ipomoea batatas L.) leaves.
J Agric Food Chem. 2007 Jan 10; 55(1):185-90.JA

Abstract

Sweetpotato leaves (Ipomoea batatas L.) contain a high content of polyphenolics that consist of caffeic acid, chlorogenic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, and 3,4,5-tri-O-caffeoylquinic acid. We investigated the suppression of the proliferation of selected human cancer cells by phenolic compounds isolated from sweetpotato leaf. The human cancer cells used in this research included a stomach cancer (Kato III), a colon cancer (DLD-1), and a promyelocytic leukemia cell (HL-60). Caffeic acid and di- and tricaffeoylquinic acids dose-dependently depressed cancer cell proliferation, and the difference in sensitivity between caffeoylquinic acid derivatives and each kind of cancer cell was observed. Specifically, 3,4,5-tri-O-caffeoylquinic acid effectively depressed the growth of three kinds of cancer cells, and caffeic acid had an exceptionally higher effect against HL-60 cells than other di- and tricaffeoylquinic acids. In attempting to clarify the mechanism of growth suppression with the addition of the apoptotic inhibitor N-ethylmaleimide, we observed that the nuclear granulation in 3,4,5-tri-O-caffeoylquinic acid-treated HL-60 cells suggested apoptosis induction. This effect was confirmed by DNA fragmentation, an increase of caspase-3 activity, and expression of c-Jun. Growth suppression of HL-60 cells by 3,4,5-tri-O-caffeoylquinic acid was determined to be the result of apoptotic death of the cells. These results indicate that 3,4,5-tri-O-caffeoylquinic acid may have potential for cancer prevention.

Authors+Show Affiliations

Department of Upland Farming Research, National Agricultural Research Center for Kyushu Okinawa Region, Yokoichi 6651-2, Miyakonojo, Miyazaki 885-0091, Japan. rkurata@affrc.go.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17199331

Citation

Kurata, Rie, et al. "Growth Suppression of Human Cancer Cells By Polyphenolics From Sweetpotato (Ipomoea Batatas L.) Leaves." Journal of Agricultural and Food Chemistry, vol. 55, no. 1, 2007, pp. 185-90.
Kurata R, Adachi M, Yamakawa O, et al. Growth suppression of human cancer cells by polyphenolics from sweetpotato (Ipomoea batatas L.) leaves. J Agric Food Chem. 2007;55(1):185-90.
Kurata, R., Adachi, M., Yamakawa, O., & Yoshimoto, M. (2007). Growth suppression of human cancer cells by polyphenolics from sweetpotato (Ipomoea batatas L.) leaves. Journal of Agricultural and Food Chemistry, 55(1), 185-90.
Kurata R, et al. Growth Suppression of Human Cancer Cells By Polyphenolics From Sweetpotato (Ipomoea Batatas L.) Leaves. J Agric Food Chem. 2007 Jan 10;55(1):185-90. PubMed PMID: 17199331.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth suppression of human cancer cells by polyphenolics from sweetpotato (Ipomoea batatas L.) leaves. AU - Kurata,Rie, AU - Adachi,Masaru, AU - Yamakawa,Osamu, AU - Yoshimoto,Makoto, PY - 2007/1/4/pubmed PY - 2007/3/29/medline PY - 2007/1/4/entrez SP - 185 EP - 90 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 55 IS - 1 N2 - Sweetpotato leaves (Ipomoea batatas L.) contain a high content of polyphenolics that consist of caffeic acid, chlorogenic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, and 3,4,5-tri-O-caffeoylquinic acid. We investigated the suppression of the proliferation of selected human cancer cells by phenolic compounds isolated from sweetpotato leaf. The human cancer cells used in this research included a stomach cancer (Kato III), a colon cancer (DLD-1), and a promyelocytic leukemia cell (HL-60). Caffeic acid and di- and tricaffeoylquinic acids dose-dependently depressed cancer cell proliferation, and the difference in sensitivity between caffeoylquinic acid derivatives and each kind of cancer cell was observed. Specifically, 3,4,5-tri-O-caffeoylquinic acid effectively depressed the growth of three kinds of cancer cells, and caffeic acid had an exceptionally higher effect against HL-60 cells than other di- and tricaffeoylquinic acids. In attempting to clarify the mechanism of growth suppression with the addition of the apoptotic inhibitor N-ethylmaleimide, we observed that the nuclear granulation in 3,4,5-tri-O-caffeoylquinic acid-treated HL-60 cells suggested apoptosis induction. This effect was confirmed by DNA fragmentation, an increase of caspase-3 activity, and expression of c-Jun. Growth suppression of HL-60 cells by 3,4,5-tri-O-caffeoylquinic acid was determined to be the result of apoptotic death of the cells. These results indicate that 3,4,5-tri-O-caffeoylquinic acid may have potential for cancer prevention. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/17199331/Growth_suppression_of_human_cancer_cells_by_polyphenolics_from_sweetpotato__Ipomoea_batatas_L___leaves_ L2 - https://doi.org/10.1021/jf0620259 DB - PRIME DP - Unbound Medicine ER -