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CD69 expression on airway eosinophils and airway inflammation in a murine model of asthma.
Chin Med J (Engl). 2006 Dec 05; 119(23):1983-90.CM

Abstract

BACKGROUND

Asthma is a chronic airway disease with inflammation characterized by physiological changes (airway hyper-responsiveness, AHR) and pathological changes (inflammatory cells infiltration and mucus production). Eosinophils play a key role in the allergic inflammation. But the causative relationship between eosinophils and airway inflammation is hard to prove. One of the reasons is lack of activation marker of murine eosinophils. We investigated the expression of CD69 on murine eosinophils in vitro, the relationship between the expression of CD69 on eosinophils from peripheral blood and bronchoalveolar lavage fluid and on airway inflammation in asthmatic mice.

METHODS

Eosinophils from peripheral blood of IL-5 transgenic mice (NJ.1638) were purified. Mice were divided into five groups: wild type mice sensitized and challenged with saline (WS group), wild type mice sensitized and challenged with ovalbumin (WO group), IL-5(-/-) mice sensitized and challenged with saline and transferred with purified eosinophils (ISE group), IL-5(-/-) mice sensitized and challenged with OVA and transferred with purified eosinophils (IOE group), IL-5(-/-) mice sensitized and challenged with OVA and transferred with purified eosinophils, pretreated with anti CD4 monoclonal antibody (IOE+antiCD4mAb group). IL-5(-/-) mice were sensitized with OVA at day 0 and day 14, then challenged with OVA aerosol. On days 24, 25, 26 and 27 purified eosinophils were transferred intratracheally to IL-5(-/-) mice. On day 28, blood and BALF were collected and CD69 expression on eosinophils measured by flowcytometry.

RESULTS

Purified eosinophils did not express CD69. But eosinophils cultured with PMA + MA, IFN-gamma, IL-5 or GM-CSF expressed CD69 strongly. Eosinophils from blood of WO, WS group did not express CD69 at all. The numbers of eosinophils in BALF of WO group, IOE group, ISE group and IOE + antiCD4mAb group were significantly higher than in mice of WS group which did not have eosinophils at all. CD69 expression on eosinophils in BALF of IOE and WO groups was strong. Eosinophils in BALF of ISE and IOE + antiCDmAb groups did not express CD69. The mucus production result was similar to CD69 expression. There were eosinophils infiltration in lung slides of all groups except WS group.

CONCLUSION

Activation in airway of eosinophils could directly lead to airway inflammation.

Authors+Show Affiliations

Department of Respiratory Medicine, Second Affiliated Hospital of Zhejiang University Medical College, Institute of Respiratory Diseases of Zhejiang University, Hangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17199943

Citation

Wang, Hui-ying, et al. "CD69 Expression On Airway Eosinophils and Airway Inflammation in a Murine Model of Asthma." Chinese Medical Journal, vol. 119, no. 23, 2006, pp. 1983-90.
Wang HY, Shen HH, Lee JJ, et al. CD69 expression on airway eosinophils and airway inflammation in a murine model of asthma. Chin Med J (Engl). 2006;119(23):1983-90.
Wang, H. Y., Shen, H. H., Lee, J. J., & Lee, N. A. (2006). CD69 expression on airway eosinophils and airway inflammation in a murine model of asthma. Chinese Medical Journal, 119(23), 1983-90.
Wang HY, et al. CD69 Expression On Airway Eosinophils and Airway Inflammation in a Murine Model of Asthma. Chin Med J (Engl). 2006 Dec 5;119(23):1983-90. PubMed PMID: 17199943.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CD69 expression on airway eosinophils and airway inflammation in a murine model of asthma. AU - Wang,Hui-ying, AU - Shen,Hua-hao, AU - Lee,James J, AU - Lee,Nancy A, PY - 2007/1/4/pubmed PY - 2007/2/24/medline PY - 2007/1/4/entrez SP - 1983 EP - 90 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 119 IS - 23 N2 - BACKGROUND: Asthma is a chronic airway disease with inflammation characterized by physiological changes (airway hyper-responsiveness, AHR) and pathological changes (inflammatory cells infiltration and mucus production). Eosinophils play a key role in the allergic inflammation. But the causative relationship between eosinophils and airway inflammation is hard to prove. One of the reasons is lack of activation marker of murine eosinophils. We investigated the expression of CD69 on murine eosinophils in vitro, the relationship between the expression of CD69 on eosinophils from peripheral blood and bronchoalveolar lavage fluid and on airway inflammation in asthmatic mice. METHODS: Eosinophils from peripheral blood of IL-5 transgenic mice (NJ.1638) were purified. Mice were divided into five groups: wild type mice sensitized and challenged with saline (WS group), wild type mice sensitized and challenged with ovalbumin (WO group), IL-5(-/-) mice sensitized and challenged with saline and transferred with purified eosinophils (ISE group), IL-5(-/-) mice sensitized and challenged with OVA and transferred with purified eosinophils (IOE group), IL-5(-/-) mice sensitized and challenged with OVA and transferred with purified eosinophils, pretreated with anti CD4 monoclonal antibody (IOE+antiCD4mAb group). IL-5(-/-) mice were sensitized with OVA at day 0 and day 14, then challenged with OVA aerosol. On days 24, 25, 26 and 27 purified eosinophils were transferred intratracheally to IL-5(-/-) mice. On day 28, blood and BALF were collected and CD69 expression on eosinophils measured by flowcytometry. RESULTS: Purified eosinophils did not express CD69. But eosinophils cultured with PMA + MA, IFN-gamma, IL-5 or GM-CSF expressed CD69 strongly. Eosinophils from blood of WO, WS group did not express CD69 at all. The numbers of eosinophils in BALF of WO group, IOE group, ISE group and IOE + antiCD4mAb group were significantly higher than in mice of WS group which did not have eosinophils at all. CD69 expression on eosinophils in BALF of IOE and WO groups was strong. Eosinophils in BALF of ISE and IOE + antiCDmAb groups did not express CD69. The mucus production result was similar to CD69 expression. There were eosinophils infiltration in lung slides of all groups except WS group. CONCLUSION: Activation in airway of eosinophils could directly lead to airway inflammation. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/17199943/CD69_expression_on_airway_eosinophils_and_airway_inflammation_in_a_murine_model_of_asthma_ L2 - https://Insights.ovid.com/pubmed?pmid=17199943 DB - PRIME DP - Unbound Medicine ER -