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Comparison of capillary zone electrophoresis and isotachophoresis determination of dimethindene enantiomers in pharmaceuticals using charged carboxyethyl-beta-cyclodextrin as a chiral selector.
Methods Find Exp Clin Pharmacol. 2006 Nov; 28(9):595-9.MF

Abstract

Charged cyclodextrin-mediated capillary electrophoretic methods (capillary zone electrophoresis [CZE] and isotachophoresis [ITP]) in a hydrodynamically closed separation system with suppressed electroosmotic flow and UV absorbance photometric detection (for CZE) or conductivity detection (for ITP) were developed for a highly effective separation and quantitation of dimethindene enantiomers in various pharmaceutical formulations (solution, gel, capsules). Optimized electrolyte systems were based on low-mobility buffer constituents (epsilon-aminocaproic acid, beta-alanine, potassium acetate, acetic acid), negatively charged (at pH > 4.5) carboxyethyl-beta-cyclodextrin (CE-beta-CD), serving as chiral selector, and methylhydroxyethylcellulose, serving as an electroosmotic flow suppressor. Complete enantioseparations of dimethindene in the presence of low concentrations of CE-beta-CD (2.5 mg/ml in CZE, 6.0 mg/ml in ITP) clearly indicated a role of charge of this chiral selector in enantioresolution. It also outlined the potential of charged CD-derivatives as chiral selectors for various CE techniques. The proposed methods were successfully validated, appraising parameters of sensitivity, linearity, precision, accuracy/recovery, and robustness, and then, they were applied to pharmaceutical samples. Consistent results obtained by both CE methods indicate their usefulness for routine use.

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Authors+Show Affiliations

Mikus P
Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic. mikus@fpharm.uniba.sk
Kubacak P
No affiliation info available
Valaskova I
No affiliation info available
Havranek E
No affiliation info available

MeSH

DimethindeneElectrophoresisElectrophoresis, CapillaryPharmaceutical PreparationsSensitivity and SpecificityStereoisomerismbeta-Cyclodextrins

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

17200724

Citation

Mikus, P, et al. "Comparison of Capillary Zone Electrophoresis and Isotachophoresis Determination of Dimethindene Enantiomers in Pharmaceuticals Using Charged Carboxyethyl-beta-cyclodextrin as a Chiral Selector." Methods and Findings in Experimental and Clinical Pharmacology, vol. 28, no. 9, 2006, pp. 595-9.
Mikus P, Kubacak P, Valaskova I, et al. Comparison of capillary zone electrophoresis and isotachophoresis determination of dimethindene enantiomers in pharmaceuticals using charged carboxyethyl-beta-cyclodextrin as a chiral selector. Methods Find Exp Clin Pharmacol. 2006;28(9):595-9.
Mikus, P., Kubacak, P., Valaskova, I., & Havranek, E. (2006). Comparison of capillary zone electrophoresis and isotachophoresis determination of dimethindene enantiomers in pharmaceuticals using charged carboxyethyl-beta-cyclodextrin as a chiral selector. Methods and Findings in Experimental and Clinical Pharmacology, 28(9), 595-9.
Mikus P, et al. Comparison of Capillary Zone Electrophoresis and Isotachophoresis Determination of Dimethindene Enantiomers in Pharmaceuticals Using Charged Carboxyethyl-beta-cyclodextrin as a Chiral Selector. Methods Find Exp Clin Pharmacol. 2006;28(9):595-9. PubMed PMID: 17200724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of capillary zone electrophoresis and isotachophoresis determination of dimethindene enantiomers in pharmaceuticals using charged carboxyethyl-beta-cyclodextrin as a chiral selector. AU - Mikus,P, AU - Kubacak,P, AU - Valaskova,I, AU - Havranek,E, PY - 2007/1/4/pubmed PY - 2007/3/3/medline PY - 2007/1/4/entrez SP - 595 EP - 9 JF - Methods and findings in experimental and clinical pharmacology JO - Methods Find Exp Clin Pharmacol VL - 28 IS - 9 N2 - Charged cyclodextrin-mediated capillary electrophoretic methods (capillary zone electrophoresis [CZE] and isotachophoresis [ITP]) in a hydrodynamically closed separation system with suppressed electroosmotic flow and UV absorbance photometric detection (for CZE) or conductivity detection (for ITP) were developed for a highly effective separation and quantitation of dimethindene enantiomers in various pharmaceutical formulations (solution, gel, capsules). Optimized electrolyte systems were based on low-mobility buffer constituents (epsilon-aminocaproic acid, beta-alanine, potassium acetate, acetic acid), negatively charged (at pH > 4.5) carboxyethyl-beta-cyclodextrin (CE-beta-CD), serving as chiral selector, and methylhydroxyethylcellulose, serving as an electroosmotic flow suppressor. Complete enantioseparations of dimethindene in the presence of low concentrations of CE-beta-CD (2.5 mg/ml in CZE, 6.0 mg/ml in ITP) clearly indicated a role of charge of this chiral selector in enantioresolution. It also outlined the potential of charged CD-derivatives as chiral selectors for various CE techniques. The proposed methods were successfully validated, appraising parameters of sensitivity, linearity, precision, accuracy/recovery, and robustness, and then, they were applied to pharmaceutical samples. Consistent results obtained by both CE methods indicate their usefulness for routine use. SN - 0379-0355 UR - https://www.unboundmedicine.com/medline/citation/17200724/Comparison_of_capillary_zone_electrophoresis_and_isotachophoresis_determination_of_dimethindene_enantiomers_in_pharmaceuticals_using_charged_carboxyethyl_beta_cyclodextrin_as_a_chiral_selector_ L2 - http://journals.prous.com/journals/servlet/xmlxsl/pk_journals.xml_summaryn_pr?p_JournalId=6&p_RefId=1037496 DB - PRIME DP - Unbound Medicine ER -

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