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Antioxidant enzyme inhibitors enhance peroxynitrite-induced cell death in U937 cells.
Mol Cell Biochem. 2007 Jul; 301(1-2):61-8.MC

Abstract

Peroxynitrite, a potent physiological inorganic toxin, is known to play a critical role in cellular oxidative damage. The protective role of antioxidant enzymes against peroxynitrite-induced oxidative damage in U937 cells was investigated in control and cells pre-treated with diethyldithiocarbamic acid, aminotriazole, and oxlalomalate, specific inhibitors of superoxide dismutase, catalase, and NADP(+)-dependent isocitrate dehydrogenase, respectively. Upon exposure to 1 mM 3-morpholinosydnomine N-ethylcarbamide (SIN-1), a generator of peroxynitrite through the reaction between nitric oxide and superoxide anion, to U937 cells, the viability was lower and the protein oxidation, lipid peroxidation and oxidative DNA damage reflected by an increase in 8-hydroxy-2'-deoxyguanosine, were higher in the inhibitor-treated cells as compared to the control cells. We also observed the significant increase in the endogenous production of reactive oxygen species, as measured by the oxidation of 2'7'-dichlorodihydrofluorescin as well as the significant decrease in the intracellular GSH level in the inhibitor-treated U937 cells upon exposure to SIN-1. These results suggest that antioxidant enzymes play an important role in cellular defense against peroxynitrite-induced cell death.

Authors+Show Affiliations

Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu, Korea.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17206381

Citation

Yang, Eun Sun, and Jeen-Woo Park. "Antioxidant Enzyme Inhibitors Enhance Peroxynitrite-induced Cell Death in U937 Cells." Molecular and Cellular Biochemistry, vol. 301, no. 1-2, 2007, pp. 61-8.
Yang ES, Park JW. Antioxidant enzyme inhibitors enhance peroxynitrite-induced cell death in U937 cells. Mol Cell Biochem. 2007;301(1-2):61-8.
Yang, E. S., & Park, J. W. (2007). Antioxidant enzyme inhibitors enhance peroxynitrite-induced cell death in U937 cells. Molecular and Cellular Biochemistry, 301(1-2), 61-8.
Yang ES, Park JW. Antioxidant Enzyme Inhibitors Enhance Peroxynitrite-induced Cell Death in U937 Cells. Mol Cell Biochem. 2007;301(1-2):61-8. PubMed PMID: 17206381.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant enzyme inhibitors enhance peroxynitrite-induced cell death in U937 cells. AU - Yang,Eun Sun, AU - Park,Jeen-Woo, Y1 - 2007/01/06/ PY - 2006/09/11/received PY - 2006/12/06/accepted PY - 2007/1/9/pubmed PY - 2007/10/3/medline PY - 2007/1/9/entrez SP - 61 EP - 8 JF - Molecular and cellular biochemistry JO - Mol Cell Biochem VL - 301 IS - 1-2 N2 - Peroxynitrite, a potent physiological inorganic toxin, is known to play a critical role in cellular oxidative damage. The protective role of antioxidant enzymes against peroxynitrite-induced oxidative damage in U937 cells was investigated in control and cells pre-treated with diethyldithiocarbamic acid, aminotriazole, and oxlalomalate, specific inhibitors of superoxide dismutase, catalase, and NADP(+)-dependent isocitrate dehydrogenase, respectively. Upon exposure to 1 mM 3-morpholinosydnomine N-ethylcarbamide (SIN-1), a generator of peroxynitrite through the reaction between nitric oxide and superoxide anion, to U937 cells, the viability was lower and the protein oxidation, lipid peroxidation and oxidative DNA damage reflected by an increase in 8-hydroxy-2'-deoxyguanosine, were higher in the inhibitor-treated cells as compared to the control cells. We also observed the significant increase in the endogenous production of reactive oxygen species, as measured by the oxidation of 2'7'-dichlorodihydrofluorescin as well as the significant decrease in the intracellular GSH level in the inhibitor-treated U937 cells upon exposure to SIN-1. These results suggest that antioxidant enzymes play an important role in cellular defense against peroxynitrite-induced cell death. SN - 0300-8177 UR - https://www.unboundmedicine.com/medline/citation/17206381/Antioxidant_enzyme_inhibitors_enhance_peroxynitrite_induced_cell_death_in_U937_cells_ DB - PRIME DP - Unbound Medicine ER -