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Eruca sativa seeds possess antioxidant activity and exert a protective effect on mercuric chloride induced renal toxicity.

Abstract

Mercuric chloride (HgCl(2)) is a well-known nephrotoxic agent. Increasing number of evidences suggest the role of oxidative stress in HgCl(2) induced nephrotoxicity. Eruca sativa is widely used in folklore medicines and has a good reputation as a remedy of renal ailments. In the present study, the antioxidant potential of ethanolic extract of E. sativa seeds was determined and its protective effect on HgCl(2) induced renal toxicity was investigated. The extract was found to possess a potent antioxidant effect, with a large amount of polyphenols and a high reducing ability. HPLC analysis of the extract revealed glucoerucin and flavonoids to be the major antioxidants present in it. E. sativa extract significantly scavenged several reactive oxygen species (ROS) and reactive nitrogen species (RNS). Feeding of the extract to rats afforded a significant protection against HgCl(2) induced renal toxicity. Subcutaneous administration of 4 mg/kg body weight HgCl(2) induced renal injury evident as a marked elevation in serum creatinine and blood urea nitrogen levels, and histopathological changes such as necrosis, oedema and congestion of stroma and glomeruli. Oxidative modulation of renal tissues following HgCl(2) exposure was evident from a significant elevation in lipid peroxidation and attenuation in glutathione (GSH) contents and activities of antioxidant enzymes viz., catalase (CAT), glutathione peroxidase (GPX), superoxide dismutase (SOD) and glutathione reductase (GR). Oral administration of E. sativa extract to rats at a dose regimen: 50-200 mg/kg body weight for 7 days prior to HgCl(2) treatment significantly and dose dependently protected against alterations in all these diagnostic parameters. The data obtained in the present study suggests E. sativa seeds to possess a potent antioxidant and renal protective activity and preclude oxidative damage inflicted to the kidney.

Authors+Show Affiliations

,

Department of Chemistry, Faculty of Science, Jamia Hamdard, Hamdard Nagar, New Delhi 110 062, India. msalamchem@yahoo.com

, , ,

Source

MeSH

Animals
Antioxidants
Blood Urea Nitrogen
Creatinine
Free Radical Scavengers
Glutathione
Histocytochemistry
Kidney
Kidney Diseases
Lipid Peroxidation
Male
Mercuric Chloride
Mercury Poisoning, Nervous System
Mustard Plant
Plant Extracts
Rats
Rats, Wistar
Seeds
Thiobarbituric Acid Reactive Substances

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17207565

Citation

Sarwar Alam, M, et al. "Eruca Sativa Seeds Possess Antioxidant Activity and Exert a Protective Effect On Mercuric Chloride Induced Renal Toxicity." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 45, no. 6, 2007, pp. 910-20.
Sarwar Alam M, Kaur G, Jabbar Z, et al. Eruca sativa seeds possess antioxidant activity and exert a protective effect on mercuric chloride induced renal toxicity. Food Chem Toxicol. 2007;45(6):910-20.
Sarwar Alam, M., Kaur, G., Jabbar, Z., Javed, K., & Athar, M. (2007). Eruca sativa seeds possess antioxidant activity and exert a protective effect on mercuric chloride induced renal toxicity. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 45(6), pp. 910-20.
Sarwar Alam M, et al. Eruca Sativa Seeds Possess Antioxidant Activity and Exert a Protective Effect On Mercuric Chloride Induced Renal Toxicity. Food Chem Toxicol. 2007;45(6):910-20. PubMed PMID: 17207565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Eruca sativa seeds possess antioxidant activity and exert a protective effect on mercuric chloride induced renal toxicity. AU - Sarwar Alam,M, AU - Kaur,Gurpreet, AU - Jabbar,Zoobi, AU - Javed,Kaleem, AU - Athar,Mohammad, Y1 - 2006/11/29/ PY - 2006/03/17/received PY - 2006/11/12/revised PY - 2006/11/21/accepted PY - 2007/1/9/pubmed PY - 2007/7/25/medline PY - 2007/1/9/entrez SP - 910 EP - 20 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem. Toxicol. VL - 45 IS - 6 N2 - Mercuric chloride (HgCl(2)) is a well-known nephrotoxic agent. Increasing number of evidences suggest the role of oxidative stress in HgCl(2) induced nephrotoxicity. Eruca sativa is widely used in folklore medicines and has a good reputation as a remedy of renal ailments. In the present study, the antioxidant potential of ethanolic extract of E. sativa seeds was determined and its protective effect on HgCl(2) induced renal toxicity was investigated. The extract was found to possess a potent antioxidant effect, with a large amount of polyphenols and a high reducing ability. HPLC analysis of the extract revealed glucoerucin and flavonoids to be the major antioxidants present in it. E. sativa extract significantly scavenged several reactive oxygen species (ROS) and reactive nitrogen species (RNS). Feeding of the extract to rats afforded a significant protection against HgCl(2) induced renal toxicity. Subcutaneous administration of 4 mg/kg body weight HgCl(2) induced renal injury evident as a marked elevation in serum creatinine and blood urea nitrogen levels, and histopathological changes such as necrosis, oedema and congestion of stroma and glomeruli. Oxidative modulation of renal tissues following HgCl(2) exposure was evident from a significant elevation in lipid peroxidation and attenuation in glutathione (GSH) contents and activities of antioxidant enzymes viz., catalase (CAT), glutathione peroxidase (GPX), superoxide dismutase (SOD) and glutathione reductase (GR). Oral administration of E. sativa extract to rats at a dose regimen: 50-200 mg/kg body weight for 7 days prior to HgCl(2) treatment significantly and dose dependently protected against alterations in all these diagnostic parameters. The data obtained in the present study suggests E. sativa seeds to possess a potent antioxidant and renal protective activity and preclude oxidative damage inflicted to the kidney. SN - 0278-6915 UR - https://www.unboundmedicine.com/medline/citation/17207565/Eruca_sativa_seeds_possess_antioxidant_activity_and_exert_a_protective_effect_on_mercuric_chloride_induced_renal_toxicity_ DB - PRIME DP - Unbound Medicine ER -