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[Characterization of low-molecular-weighT i-type glutenin subunit genes from diploid wheat in relation to the gene family structure].
Mol Biol (Mosk). 2006 Nov-Dec; 40(6):996-1005.MB

Abstract

The low-molecular-weight (LMW) glutenin subunits are important for aspects of wheat quality and dough processing, and the LMW-i type glutenin is one of the typical glutenins. However, a detailed description of the DNA structure and encoded polypeptides of the LMW-i type glutenin subunit gene family is still lacking. In this study, two LMW-i type glutenin subunit genes, i.e., LMW-Eb from Triticum boeoticum and LMW-Em from T. monococcum, were obtained from genomic DNA, respectively. The LMW-Eb is a novel gene and the LMW-Em has the identical sequence with a known gene. To comprehensively understand the LMW-i type glutenin subunit gene family structure, all known LMW-i type glutenin subunit genes were comparatively analyzed. Detailed comparison of these genes revealed a high-level of single nucleotide polymorphisms (SNP). In these LMW glutenin subunits, the percentage of glutamine and proline were approximately 38.27 and 12.77%, respectively. They started directly from the repetitive domain with ISQQQ- after the signal sequence, which the N-terminal regions were absent. In addition, there are three consensus repeat motifs (i.e. PPFSQQQQ, PPISQQQQ and PPYSQQQQ) in the repetitive domains of these LMW glutenin subunits. The C-terminal I domain is the most conserved region, while the domains of C-terminal II and III are more variable. The eight cysteine residues are highly conserved. These genes could be clustered into two major groups, among which one group could be further divided into five subgroups. Furthermore, to date, all known LMW-i type glutenin subunit genes are located on chromosome 1A, whereas no LMW-i type glutenin subunit gene is obtained from the B and D genomes in wheat.

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Authors

Ma ZC
No affiliation info available
Wei YM
No affiliation info available
Long H
No affiliation info available
Yan ZH
No affiliation info available
Baum B
No affiliation info available
Zheng YL
No affiliation info available

MeSH

Amino Acid SequenceBase SequenceCloning, MolecularDiploidyGenes, PlantGlutensMolecular Sequence DataMolecular WeightMultigene FamilyPhylogenyProtein Structure, TertiaryProtein SubunitsSequence AlignmentTriticum

Pub Type(s)

Journal Article

Language

rus

PubMed ID

17209427

Citation

Ma, Zhao-Cai, et al. "[Characterization of low-molecular-weighT I-type Glutenin Subunit Genes From Diploid Wheat in Relation to the Gene Family Structure]." Molekuliarnaia Biologiia, vol. 40, no. 6, 2006, pp. 996-1005.
Ma ZC, Wei YM, Long H, et al. [Characterization of low-molecular-weighT i-type glutenin subunit genes from diploid wheat in relation to the gene family structure]. Mol Biol (Mosk). 2006;40(6):996-1005.
Ma, Z. C., Wei, Y. M., Long, H., Yan, Z. H., Baum, B., & Zheng, Y. L. (2006). [Characterization of low-molecular-weighT i-type glutenin subunit genes from diploid wheat in relation to the gene family structure]. Molekuliarnaia Biologiia, 40(6), 996-1005.
Ma ZC, et al. [Characterization of low-molecular-weighT I-type Glutenin Subunit Genes From Diploid Wheat in Relation to the Gene Family Structure]. Mol Biol (Mosk). 2006 Nov-Dec;40(6):996-1005. PubMed PMID: 17209427.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Characterization of low-molecular-weighT i-type glutenin subunit genes from diploid wheat in relation to the gene family structure]. AU - Ma,Zhao-Cai, AU - Wei,Yu-Ming, AU - Long,Hai, AU - Yan,Ze-Hong, AU - Baum,Bernard, AU - Zheng,You-Liang, PY - 2007/1/11/pubmed PY - 2007/2/17/medline PY - 2007/1/11/entrez SP - 996 EP - 1005 JF - Molekuliarnaia biologiia JO - Mol Biol (Mosk) VL - 40 IS - 6 N2 - The low-molecular-weight (LMW) glutenin subunits are important for aspects of wheat quality and dough processing, and the LMW-i type glutenin is one of the typical glutenins. However, a detailed description of the DNA structure and encoded polypeptides of the LMW-i type glutenin subunit gene family is still lacking. In this study, two LMW-i type glutenin subunit genes, i.e., LMW-Eb from Triticum boeoticum and LMW-Em from T. monococcum, were obtained from genomic DNA, respectively. The LMW-Eb is a novel gene and the LMW-Em has the identical sequence with a known gene. To comprehensively understand the LMW-i type glutenin subunit gene family structure, all known LMW-i type glutenin subunit genes were comparatively analyzed. Detailed comparison of these genes revealed a high-level of single nucleotide polymorphisms (SNP). In these LMW glutenin subunits, the percentage of glutamine and proline were approximately 38.27 and 12.77%, respectively. They started directly from the repetitive domain with ISQQQ- after the signal sequence, which the N-terminal regions were absent. In addition, there are three consensus repeat motifs (i.e. PPFSQQQQ, PPISQQQQ and PPYSQQQQ) in the repetitive domains of these LMW glutenin subunits. The C-terminal I domain is the most conserved region, while the domains of C-terminal II and III are more variable. The eight cysteine residues are highly conserved. These genes could be clustered into two major groups, among which one group could be further divided into five subgroups. Furthermore, to date, all known LMW-i type glutenin subunit genes are located on chromosome 1A, whereas no LMW-i type glutenin subunit gene is obtained from the B and D genomes in wheat. SN - 0026-8984 UR - https://www.unboundmedicine.com/medline/citation/17209427/[Characterization_of_low_molecular_weighT_i_type_glutenin_subunit_genes_from_diploid_wheat_in_relation_to_the_gene_family_structure]_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=17209427.ui DB - PRIME DP - Unbound Medicine ER -