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Combined Fontana-Masson-mucin staining of Cryptococcus neoformans.
Arch Pathol Lab Med. 1991 Nov; 115(11):1145-9.AP

Abstract

Cryptococci react positively with various histochemical stains, including the Fontana-Masson (FM), which stains the cell wall, and mucin stains, such as alcian blue and mucicarmine, which stain the capsule. Combinations of the FM stain with both the alcian blue and mucicarmine stains were performed on paraffin-embedded tissue specimens that were obtained from 15 patients who had culture-proved cryptococcosis. Combined FM-mucicarmine and FM-alcian blue stains were compared with other individual fungal stains. The FM stain, followed by either the mucicarmine or alcian blue stain, distinctively demonstrated both the cell wall and capsule of most organisms. More organisms were recognized in the combined stains than with either stain done individually. No interference between the stains was noted. Combining the FM stain with either of these two mucin stains appears to be helpful for identifying cryptococci.

Authors+Show Affiliations

Lazcano O
Department of Anatomical Pathology, Scott, Sherwood and Brindley Foundation, Texas A&M University College of Medicine, Scott and White Clinic, Temple 76508.
Speights VO
No affiliation info available
Bilbao J
No affiliation info available
Becker J
No affiliation info available
Diaz J
No affiliation info available

MeSH

Alcian BlueCarmineColoring AgentsCryptococcosisCryptococcus neoformansHumansPeriodic Acid-Schiff ReactionSensitivity and SpecificitySilver NitrateSilver StainingStaining and Labeling

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

1720948

Citation

Lazcano, O, et al. "Combined Fontana-Masson-mucin Staining of Cryptococcus Neoformans." Archives of Pathology & Laboratory Medicine, vol. 115, no. 11, 1991, pp. 1145-9.
Lazcano O, Speights VO, Bilbao J, et al. Combined Fontana-Masson-mucin staining of Cryptococcus neoformans. Arch Pathol Lab Med. 1991;115(11):1145-9.
Lazcano, O., Speights, V. O., Bilbao, J., Becker, J., & Diaz, J. (1991). Combined Fontana-Masson-mucin staining of Cryptococcus neoformans. Archives of Pathology & Laboratory Medicine, 115(11), 1145-9.
Lazcano O, et al. Combined Fontana-Masson-mucin Staining of Cryptococcus Neoformans. Arch Pathol Lab Med. 1991;115(11):1145-9. PubMed PMID: 1720948.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combined Fontana-Masson-mucin staining of Cryptococcus neoformans. AU - Lazcano,O, AU - Speights,V O,Jr AU - Bilbao,J, AU - Becker,J, AU - Diaz,J, PY - 1991/11/11/pubmed PY - 2001/3/28/medline PY - 1991/11/11/entrez SP - 1145 EP - 9 JF - Archives of pathology & laboratory medicine JO - Arch Pathol Lab Med VL - 115 IS - 11 N2 - Cryptococci react positively with various histochemical stains, including the Fontana-Masson (FM), which stains the cell wall, and mucin stains, such as alcian blue and mucicarmine, which stain the capsule. Combinations of the FM stain with both the alcian blue and mucicarmine stains were performed on paraffin-embedded tissue specimens that were obtained from 15 patients who had culture-proved cryptococcosis. Combined FM-mucicarmine and FM-alcian blue stains were compared with other individual fungal stains. The FM stain, followed by either the mucicarmine or alcian blue stain, distinctively demonstrated both the cell wall and capsule of most organisms. More organisms were recognized in the combined stains than with either stain done individually. No interference between the stains was noted. Combining the FM stain with either of these two mucin stains appears to be helpful for identifying cryptococci. SN - 0003-9985 UR - https://www.unboundmedicine.com/medline/citation/1720948/Combined_Fontana_Masson_mucin_staining_of_Cryptococcus_neoformans_ DB - PRIME DP - Unbound Medicine ER -