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Immunohistochemical characterization of slow and fast myosin heavy chain composition of muscle fibres in the styloglossus muscle of the human and macaque (Macaca rhesus).
Arch Oral Biol. 2007 Jun; 52(6):533-43.AO

Abstract

OBJECTIVE

Muscle fibre contractile diversity is thought to be increased by the hybridization of multiple myosin heavy chain (MHC) isoforms in single muscle fibres. Reports of hybrid fibres composed of MHCI and MHCII isoforms in human, but not macaque, tongue muscles, suggest a human adaptation for increased tongue muscle contractile diversity. Here we test whether hybrid fibres composed of MHCI and MHCII are unique to human tongue muscles or are present as well in the macaque.

METHODS

MHC composition of the macaque and human styloglossus was characterized with antibodies that allowed identification of three muscle fibre phenotypes, a slow phenotype composed of MHCI, a fast phenotype composed of MHCII and a hybrid phenotype composed of MHCI and MHCII.

RESULTS

The fast phenotype constitutes 68.5% of fibres in the macaque and 43.4% of fibres in the human (P<0.0001). The slow phenotype constitutes 20.2% of fibres in the macaque and 39.3% of fibres in the human (P<0.0001). The hybrid phenotype constitutes 11.2% of fibres in the macaque and 17.3% of fibres in the human (P=0.0002). Macaques and humans do not differ in fiber size (cross-sectional area, diameter). However, measures of fibre size differ by phenotype such that fast>hybrid>slow (P<0.05).

CONCLUSION

These data demonstrate differences in the relative percent of muscle fibre phenotypes in the macaque and human styloglossus but also demonstrate that all three phenotypes are present in both species. These data suggest a similar range of mechanical properties in styloglossus muscle fibres of the macaque and human.

Authors+Show Affiliations

Department of Physiology, Emory University School of Medicine, Atlanta, GA 30322, USA. sokoloff@physio.emory.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17210117

Citation

Sokoloff, Alan J., et al. "Immunohistochemical Characterization of Slow and Fast Myosin Heavy Chain Composition of Muscle Fibres in the Styloglossus Muscle of the Human and Macaque (Macaca Rhesus)." Archives of Oral Biology, vol. 52, no. 6, 2007, pp. 533-43.
Sokoloff AJ, Yang B, Li H, et al. Immunohistochemical characterization of slow and fast myosin heavy chain composition of muscle fibres in the styloglossus muscle of the human and macaque (Macaca rhesus). Arch Oral Biol. 2007;52(6):533-43.
Sokoloff, A. J., Yang, B., Li, H., & Burkholder, T. J. (2007). Immunohistochemical characterization of slow and fast myosin heavy chain composition of muscle fibres in the styloglossus muscle of the human and macaque (Macaca rhesus). Archives of Oral Biology, 52(6), 533-43.
Sokoloff AJ, et al. Immunohistochemical Characterization of Slow and Fast Myosin Heavy Chain Composition of Muscle Fibres in the Styloglossus Muscle of the Human and Macaque (Macaca Rhesus). Arch Oral Biol. 2007;52(6):533-43. PubMed PMID: 17210117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunohistochemical characterization of slow and fast myosin heavy chain composition of muscle fibres in the styloglossus muscle of the human and macaque (Macaca rhesus). AU - Sokoloff,Alan J, AU - Yang,Betty, AU - Li,Haiyan, AU - Burkholder,Thomas J, Y1 - 2007/01/08/ PY - 2006/08/10/received PY - 2006/10/09/revised PY - 2006/11/16/accepted PY - 2007/1/11/pubmed PY - 2008/1/3/medline PY - 2007/1/11/entrez SP - 533 EP - 43 JF - Archives of oral biology JO - Arch Oral Biol VL - 52 IS - 6 N2 - OBJECTIVE: Muscle fibre contractile diversity is thought to be increased by the hybridization of multiple myosin heavy chain (MHC) isoforms in single muscle fibres. Reports of hybrid fibres composed of MHCI and MHCII isoforms in human, but not macaque, tongue muscles, suggest a human adaptation for increased tongue muscle contractile diversity. Here we test whether hybrid fibres composed of MHCI and MHCII are unique to human tongue muscles or are present as well in the macaque. METHODS: MHC composition of the macaque and human styloglossus was characterized with antibodies that allowed identification of three muscle fibre phenotypes, a slow phenotype composed of MHCI, a fast phenotype composed of MHCII and a hybrid phenotype composed of MHCI and MHCII. RESULTS: The fast phenotype constitutes 68.5% of fibres in the macaque and 43.4% of fibres in the human (P<0.0001). The slow phenotype constitutes 20.2% of fibres in the macaque and 39.3% of fibres in the human (P<0.0001). The hybrid phenotype constitutes 11.2% of fibres in the macaque and 17.3% of fibres in the human (P=0.0002). Macaques and humans do not differ in fiber size (cross-sectional area, diameter). However, measures of fibre size differ by phenotype such that fast>hybrid>slow (P<0.05). CONCLUSION: These data demonstrate differences in the relative percent of muscle fibre phenotypes in the macaque and human styloglossus but also demonstrate that all three phenotypes are present in both species. These data suggest a similar range of mechanical properties in styloglossus muscle fibres of the macaque and human. SN - 0003-9969 UR - https://www.unboundmedicine.com/medline/citation/17210117/Immunohistochemical_characterization_of_slow_and_fast_myosin_heavy_chain_composition_of_muscle_fibres_in_the_styloglossus_muscle_of_the_human_and_macaque__Macaca_rhesus__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9969(06)00311-6 DB - PRIME DP - Unbound Medicine ER -