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Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones.
J Endocrinol 2007; 192(1):249-60JE

Abstract

Effects of salinity and hormones on cystic fibrosis transmembrane conductance regulator (CFTR) and alpha-subunit Na(+),K(+) -ATPase (alpha-NKA) mRNA (analysed by semi-quantitative PCR) and protein expression (analysed by western blotting and immunocytochemistry) were investigated in gills of striped bass. Freshwater (FW) to seawater (SW) transfer induced a disturbance in serum [Na(+)]. Gill CFTR protein, mRNA level and Na(+),K(+) -ATPase activity were unaffected by SW transfer, whereas alpha-NKA mRNA increased after transfer. CFTR immunoreactivity was observed in large cells in FW and SW gill filaments at equal intensity. Cortisol decreased serum [Na(+)] in FW fish, but had no effect on gill Na(+),K(+) -ATPase activity, alpha-NKA and CFTR mRNA levels. Incubation of gill tissue with cortisol (24 h, >0.01 micro g/ml) and epidermal growth factor (EGF 10 micro g/ml) decreased CFTR mRNA levels relative to pre-incubation and control levels. CFTR expression was unaffected by IGF-I (10 micro g/ml). alpha-NKA mRNA levels decreased by 50% after 24 h control incubation; it was slightly stimulated by cortisol and unaffected by IGF-I and EGF. In isolated gill cells, phosphorylation of extracellular-regulated kinase (ERK) 1/2 was stimulated by EGF but not affected by IGF-I. This study is the first to report a branchial EGF response and to demonstrate a functional ERK 1/2 pathway in the teleost gill. In conclusion, CFTR and Na(+),K(+) -ATPase are differentially regulated by salinity and hormones in gills of striped bass, despite the putative involvement of both in salt excretion.

Authors+Show Affiliations

Institute of Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. steffen@biology.sdu.dkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17210762

Citation

Madsen, Steffen Søndergaard, et al. "Differential Regulation of Cystic Fibrosis Transmembrane Conductance Regulator and Na+,K+ -ATPase in Gills of Striped Bass, Morone Saxatilis: Effect of Salinity and Hormones." The Journal of Endocrinology, vol. 192, no. 1, 2007, pp. 249-60.
Madsen SS, Jensen LN, Tipsmark CK, et al. Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones. J Endocrinol. 2007;192(1):249-60.
Madsen, S. S., Jensen, L. N., Tipsmark, C. K., Kiilerich, P., & Borski, R. J. (2007). Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones. The Journal of Endocrinology, 192(1), pp. 249-60.
Madsen SS, et al. Differential Regulation of Cystic Fibrosis Transmembrane Conductance Regulator and Na+,K+ -ATPase in Gills of Striped Bass, Morone Saxatilis: Effect of Salinity and Hormones. J Endocrinol. 2007;192(1):249-60. PubMed PMID: 17210762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones. AU - Madsen,Steffen Søndergaard, AU - Jensen,Lars Nørholm, AU - Tipsmark,Christian Kølbaek, AU - Kiilerich,Pia, AU - Borski,Russell John, PY - 2007/1/11/pubmed PY - 2007/4/14/medline PY - 2007/1/11/entrez SP - 249 EP - 60 JF - The Journal of endocrinology JO - J. Endocrinol. VL - 192 IS - 1 N2 - Effects of salinity and hormones on cystic fibrosis transmembrane conductance regulator (CFTR) and alpha-subunit Na(+),K(+) -ATPase (alpha-NKA) mRNA (analysed by semi-quantitative PCR) and protein expression (analysed by western blotting and immunocytochemistry) were investigated in gills of striped bass. Freshwater (FW) to seawater (SW) transfer induced a disturbance in serum [Na(+)]. Gill CFTR protein, mRNA level and Na(+),K(+) -ATPase activity were unaffected by SW transfer, whereas alpha-NKA mRNA increased after transfer. CFTR immunoreactivity was observed in large cells in FW and SW gill filaments at equal intensity. Cortisol decreased serum [Na(+)] in FW fish, but had no effect on gill Na(+),K(+) -ATPase activity, alpha-NKA and CFTR mRNA levels. Incubation of gill tissue with cortisol (24 h, >0.01 micro g/ml) and epidermal growth factor (EGF 10 micro g/ml) decreased CFTR mRNA levels relative to pre-incubation and control levels. CFTR expression was unaffected by IGF-I (10 micro g/ml). alpha-NKA mRNA levels decreased by 50% after 24 h control incubation; it was slightly stimulated by cortisol and unaffected by IGF-I and EGF. In isolated gill cells, phosphorylation of extracellular-regulated kinase (ERK) 1/2 was stimulated by EGF but not affected by IGF-I. This study is the first to report a branchial EGF response and to demonstrate a functional ERK 1/2 pathway in the teleost gill. In conclusion, CFTR and Na(+),K(+) -ATPase are differentially regulated by salinity and hormones in gills of striped bass, despite the putative involvement of both in salt excretion. SN - 0022-0795 UR - https://www.unboundmedicine.com/medline/citation/17210762/Differential_regulation_of_cystic_fibrosis_transmembrane_conductance_regulator_and_Na+K+__ATPase_in_gills_of_striped_bass_Morone_saxatilis:_effect_of_salinity_and_hormones_ L2 - https://joe.bioscientifica.com/doi/10.1677/JOE-06-0016 DB - PRIME DP - Unbound Medicine ER -