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Real-time quantification of human telomerase reverse transcriptase mRNA in liver tissues from patients with hepatocellular cancer and chronic viral hepatitis.
J Viral Hepat. 2007 Jan; 14(1):41-7.JV

Abstract

We determined, for the first time, the human telomerase reverse transcriptase (hTERT) mRNA expression, using real-time quantitative PCR, in liver tissues from patients with hepatocellular cancer (HCC; n = 13), chronic hepatitis B (n = 19) and C (n = 13). Liver tissues from the 45 patients and 17 patients without liver disease in whom liver biopsy was performed during cholecystectomy (control group), were investigated for telomerase activity (TA) and hTERT mRNA expression using the LightCycler technology. TA was detected in all HCC tissues compared with 15.6% of chronic hepatitis (P < 0.001) and none of controls (P < 0.001). TA levels and hTERT mRNA were higher in HCC compared with chronic hepatitis (P < 0.001) and normal livers (P < 0.001). hTERT mRNA expression was correlated with TA (P < 0.05). Chronic hepatitis patients who tested negative for TA and hTERT mRNA had significantly lower disease duration (58 +/- 85 months) compared with those tested positive (144 +/- 50 months; P < 0.05). Detection of TA and quantification of hTERT mRNA expression in liver tissues could be useful and additional markers for HCC diagnosis and may serve as prognostic markers for HCC development in chronic viral hepatitis patients. However, we were not able to draw general conclusions at this moment, as the number of chronic hepatitis patients positive for hTERT mRNA was relatively small. Real-time quantification of hTERT mRNA expression as a diagnostic/prognostic marker in patients with chronic hepatitis B and C and its relationship with hepatocarcinogenesis needs further evaluation.

Authors+Show Affiliations

Department of Biology, Medical School, University of Thessaly, Larissa, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17212643

Citation

Satra, M, et al. "Real-time Quantification of Human Telomerase Reverse Transcriptase mRNA in Liver Tissues From Patients With Hepatocellular Cancer and Chronic Viral Hepatitis." Journal of Viral Hepatitis, vol. 14, no. 1, 2007, pp. 41-7.
Satra M, Gatselis N, Iliopoulos D, et al. Real-time quantification of human telomerase reverse transcriptase mRNA in liver tissues from patients with hepatocellular cancer and chronic viral hepatitis. J Viral Hepat. 2007;14(1):41-7.
Satra, M., Gatselis, N., Iliopoulos, D., Zacharoulis, D., Dalekos, G. N., & Tsezou, A. (2007). Real-time quantification of human telomerase reverse transcriptase mRNA in liver tissues from patients with hepatocellular cancer and chronic viral hepatitis. Journal of Viral Hepatitis, 14(1), 41-7.
Satra M, et al. Real-time Quantification of Human Telomerase Reverse Transcriptase mRNA in Liver Tissues From Patients With Hepatocellular Cancer and Chronic Viral Hepatitis. J Viral Hepat. 2007;14(1):41-7. PubMed PMID: 17212643.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Real-time quantification of human telomerase reverse transcriptase mRNA in liver tissues from patients with hepatocellular cancer and chronic viral hepatitis. AU - Satra,M, AU - Gatselis,N, AU - Iliopoulos,D, AU - Zacharoulis,D, AU - Dalekos,G N, AU - Tsezou,A, PY - 2007/1/11/pubmed PY - 2007/3/21/medline PY - 2007/1/11/entrez SP - 41 EP - 7 JF - Journal of viral hepatitis JO - J Viral Hepat VL - 14 IS - 1 N2 - We determined, for the first time, the human telomerase reverse transcriptase (hTERT) mRNA expression, using real-time quantitative PCR, in liver tissues from patients with hepatocellular cancer (HCC; n = 13), chronic hepatitis B (n = 19) and C (n = 13). Liver tissues from the 45 patients and 17 patients without liver disease in whom liver biopsy was performed during cholecystectomy (control group), were investigated for telomerase activity (TA) and hTERT mRNA expression using the LightCycler technology. TA was detected in all HCC tissues compared with 15.6% of chronic hepatitis (P < 0.001) and none of controls (P < 0.001). TA levels and hTERT mRNA were higher in HCC compared with chronic hepatitis (P < 0.001) and normal livers (P < 0.001). hTERT mRNA expression was correlated with TA (P < 0.05). Chronic hepatitis patients who tested negative for TA and hTERT mRNA had significantly lower disease duration (58 +/- 85 months) compared with those tested positive (144 +/- 50 months; P < 0.05). Detection of TA and quantification of hTERT mRNA expression in liver tissues could be useful and additional markers for HCC diagnosis and may serve as prognostic markers for HCC development in chronic viral hepatitis patients. However, we were not able to draw general conclusions at this moment, as the number of chronic hepatitis patients positive for hTERT mRNA was relatively small. Real-time quantification of hTERT mRNA expression as a diagnostic/prognostic marker in patients with chronic hepatitis B and C and its relationship with hepatocarcinogenesis needs further evaluation. SN - 1352-0504 UR - https://www.unboundmedicine.com/medline/citation/17212643/Real_time_quantification_of_human_telomerase_reverse_transcriptase_mRNA_in_liver_tissues_from_patients_with_hepatocellular_cancer_and_chronic_viral_hepatitis_ L2 - https://doi.org/10.1111/j.1365-2893.2006.00769.x DB - PRIME DP - Unbound Medicine ER -