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The heat stability of Plasmodium lactate dehydrogenase-based and histidine-rich protein 2-based malaria rapid diagnostic tests.
Trans R Soc Trop Med Hyg. 2007 Apr; 101(4):331-7.TR

Abstract

Malaria rapid diagnostic tests (RDTs) have performed well in a variety of studies, but recent reports have described sensitivity for Plasmodium falciparum as significantly lower than that required for operational deployment. Exposure to high temperature has been suggested as an explanation. This study assessed the temperature stability of two different Plasmodium lactate dehydrogenase (pLDH)- and three histidine-rich protein 2 (HRP2)-detecting RDTs. One HRP2 test proved insufficiently sensitive for assessment. After incubation at 35, 45 and 60 degrees C, two RDTs detecting pLDH showed a substantial fall in percentage test line positivity over time, which was not seen with the remaining two HRP-2-based RDTs. For the particular products studied, variability was high, with the pLDH-based RDTs being less sensitive than HRP2-based RDTs against the sample of P. falciparum used and more susceptible to heat-induced damage, but the reasons for this are unclear. The performance of malaria RDTs can be adversely affected at the temperatures to which they will be exposed when transported to, and used in, the rural tropics.

Links

Publisher Full Text
linkinghub.elsevier.com
academic.oup.com

Authors+Show Affiliations

Chiodini PL
Department of Clinical Parasitology, Hospital for Tropical Diseases, London, UK. peter.chiodini@uclh.nhs.uk
Bowers K
No affiliation info available
Jorgensen P
No affiliation info available
Barnwell JW
No affiliation info available
Grady KK
No affiliation info available
Luchavez J
No affiliation info available
Moody AH
No affiliation info available
Cenizal A
No affiliation info available
Bell D
No affiliation info available

MeSH

AnimalsAntigens, ProtozoanHot TemperatureL-Lactate DehydrogenaseMalaria, FalciparumPlasmodium falciparumProtozoan ProteinsReagent Kits, DiagnosticTemperatureTropical Climate

Pub Type(s)

Evaluation Study
Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17212967

Citation

Chiodini, Peter L., et al. "The Heat Stability of Plasmodium Lactate Dehydrogenase-based and Histidine-rich Protein 2-based Malaria Rapid Diagnostic Tests." Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 101, no. 4, 2007, pp. 331-7.
Chiodini PL, Bowers K, Jorgensen P, et al. The heat stability of Plasmodium lactate dehydrogenase-based and histidine-rich protein 2-based malaria rapid diagnostic tests. Trans R Soc Trop Med Hyg. 2007;101(4):331-7.
Chiodini, P. L., Bowers, K., Jorgensen, P., Barnwell, J. W., Grady, K. K., Luchavez, J., Moody, A. H., Cenizal, A., & Bell, D. (2007). The heat stability of Plasmodium lactate dehydrogenase-based and histidine-rich protein 2-based malaria rapid diagnostic tests. Transactions of the Royal Society of Tropical Medicine and Hygiene, 101(4), 331-7.
Chiodini PL, et al. The Heat Stability of Plasmodium Lactate Dehydrogenase-based and Histidine-rich Protein 2-based Malaria Rapid Diagnostic Tests. Trans R Soc Trop Med Hyg. 2007;101(4):331-7. PubMed PMID: 17212967.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The heat stability of Plasmodium lactate dehydrogenase-based and histidine-rich protein 2-based malaria rapid diagnostic tests. AU - Chiodini,Peter L, AU - Bowers,Katherine, AU - Jorgensen,Pernille, AU - Barnwell,John W, AU - Grady,Katharine K, AU - Luchavez,Jenny, AU - Moody,Anthony H, AU - Cenizal,Audie, AU - Bell,David, Y1 - 2007/01/08/ PY - 2006/03/06/received PY - 2006/09/01/revised PY - 2006/09/26/accepted PY - 2007/1/11/pubmed PY - 2007/10/20/medline PY - 2007/1/11/entrez SP - 331 EP - 7 JF - Transactions of the Royal Society of Tropical Medicine and Hygiene JO - Trans R Soc Trop Med Hyg VL - 101 IS - 4 N2 - Malaria rapid diagnostic tests (RDTs) have performed well in a variety of studies, but recent reports have described sensitivity for Plasmodium falciparum as significantly lower than that required for operational deployment. Exposure to high temperature has been suggested as an explanation. This study assessed the temperature stability of two different Plasmodium lactate dehydrogenase (pLDH)- and three histidine-rich protein 2 (HRP2)-detecting RDTs. One HRP2 test proved insufficiently sensitive for assessment. After incubation at 35, 45 and 60 degrees C, two RDTs detecting pLDH showed a substantial fall in percentage test line positivity over time, which was not seen with the remaining two HRP-2-based RDTs. For the particular products studied, variability was high, with the pLDH-based RDTs being less sensitive than HRP2-based RDTs against the sample of P. falciparum used and more susceptible to heat-induced damage, but the reasons for this are unclear. The performance of malaria RDTs can be adversely affected at the temperatures to which they will be exposed when transported to, and used in, the rural tropics. SN - 0035-9203 UR - https://www.unboundmedicine.com/medline/citation/17212967/The_heat_stability_of_Plasmodium_lactate_dehydrogenase_based_and_histidine_rich_protein_2_based_malaria_rapid_diagnostic_tests_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0035-9203(06)00326-9 DB - PRIME DP - Unbound Medicine ER -