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Amperometric glucose biosensor based on multilayer films via layer-by-layer self-assembly of multi-wall carbon nanotubes, gold nanoparticles and glucose oxidase on the Pt electrode.
Biosens Bioelectron. 2007 Jun 15; 22(12):2854-60.BB

Abstract

A novel amperometric glucose biosensor based on the nine layers of multilayer films composed of multi-wall carbon nanotubes (MWCNTs), gold nanoparticles (GNp) and glucose oxidase (GOD) was developed for the specific detection of glucose. MWCNTs were chemically modified with the H(2)SO(4)-HNO(3) pretreatment to introduce carboxyl groups which were used to interact with the amino groups of poly(allylamine) (PAA) and cysteamine via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide cross-linking reaction, respectively. A cleaned Pt electrode was immersed in PAA, MWCNTs, cysteamine and GNp, respectively, followed by the adsorption of GOD, assembling the one layer of multilayer films on the surface of Pt electrode (GOD/GNp/MWCNTs/Pt electrode). Repeating the above process could assemble different layers of multilayer films on the Pt electrode. PBS washing was applied at the end of each assembly deposition for dissociating the weak adsorption. Film assembling and characterization were studied by transmission electron microscopy and quartz crystal microbalance, and properties of the resulting glucose biosensors were measured by electrochemical measurements. The marked electrocatalytic activity of Pt electrode based on multilayer films toward H(2)O(2) produced during GOD enzymatic reactions with glucose permitted effective low-potential amperometric measurement of glucose. Taking the sensitivity and selectivity into consideration, the applied potential of 0.35 V versus Ag/AgCl was chosen for the oxidation detection of H(2)O(2) in this work. Among the resulting glucose biosensors, the biosensor based on nine layers of multilayer films was best. It showed a wide linear range of 0.1-10mM glucose, with a remarkable sensitivity of 2.527 microA/mM, a detection limit of 6.7 microM estimated at a signal-to-noise ratio of 3 and fast response time (within 7s). Moreover, it exhibited good reproducibility, long-term stability and the negligible interferences of ascorbic acid, uric acid and acetaminophen. The study can provide a feasible approach on developing new kinds of oxidase-based amperometric biosensors, and can be used as an illustration for constructing various hybrid structures.

Authors+Show Affiliations

The key Laboratory of Bioactive Materials Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17212983

Citation

Wu, Bao-Yan, et al. "Amperometric Glucose Biosensor Based On Multilayer Films Via Layer-by-layer Self-assembly of Multi-wall Carbon Nanotubes, Gold Nanoparticles and Glucose Oxidase On the Pt Electrode." Biosensors & Bioelectronics, vol. 22, no. 12, 2007, pp. 2854-60.
Wu BY, Hou SH, Yin F, et al. Amperometric glucose biosensor based on multilayer films via layer-by-layer self-assembly of multi-wall carbon nanotubes, gold nanoparticles and glucose oxidase on the Pt electrode. Biosens Bioelectron. 2007;22(12):2854-60.
Wu, B. Y., Hou, S. H., Yin, F., Zhao, Z. X., Wang, Y. Y., Wang, X. S., & Chen, Q. (2007). Amperometric glucose biosensor based on multilayer films via layer-by-layer self-assembly of multi-wall carbon nanotubes, gold nanoparticles and glucose oxidase on the Pt electrode. Biosensors & Bioelectronics, 22(12), 2854-60.
Wu BY, et al. Amperometric Glucose Biosensor Based On Multilayer Films Via Layer-by-layer Self-assembly of Multi-wall Carbon Nanotubes, Gold Nanoparticles and Glucose Oxidase On the Pt Electrode. Biosens Bioelectron. 2007 Jun 15;22(12):2854-60. PubMed PMID: 17212983.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amperometric glucose biosensor based on multilayer films via layer-by-layer self-assembly of multi-wall carbon nanotubes, gold nanoparticles and glucose oxidase on the Pt electrode. AU - Wu,Bao-Yan, AU - Hou,Shi-Hua, AU - Yin,Feng, AU - Zhao,Zi-Xia, AU - Wang,Yan-Yan, AU - Wang,Xin-Sheng, AU - Chen,Qiang, Y1 - 2007/01/08/ PY - 2006/08/02/received PY - 2006/10/25/revised PY - 2006/11/20/accepted PY - 2007/1/11/pubmed PY - 2007/7/27/medline PY - 2007/1/11/entrez SP - 2854 EP - 60 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 12 N2 - A novel amperometric glucose biosensor based on the nine layers of multilayer films composed of multi-wall carbon nanotubes (MWCNTs), gold nanoparticles (GNp) and glucose oxidase (GOD) was developed for the specific detection of glucose. MWCNTs were chemically modified with the H(2)SO(4)-HNO(3) pretreatment to introduce carboxyl groups which were used to interact with the amino groups of poly(allylamine) (PAA) and cysteamine via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide cross-linking reaction, respectively. A cleaned Pt electrode was immersed in PAA, MWCNTs, cysteamine and GNp, respectively, followed by the adsorption of GOD, assembling the one layer of multilayer films on the surface of Pt electrode (GOD/GNp/MWCNTs/Pt electrode). Repeating the above process could assemble different layers of multilayer films on the Pt electrode. PBS washing was applied at the end of each assembly deposition for dissociating the weak adsorption. Film assembling and characterization were studied by transmission electron microscopy and quartz crystal microbalance, and properties of the resulting glucose biosensors were measured by electrochemical measurements. The marked electrocatalytic activity of Pt electrode based on multilayer films toward H(2)O(2) produced during GOD enzymatic reactions with glucose permitted effective low-potential amperometric measurement of glucose. Taking the sensitivity and selectivity into consideration, the applied potential of 0.35 V versus Ag/AgCl was chosen for the oxidation detection of H(2)O(2) in this work. Among the resulting glucose biosensors, the biosensor based on nine layers of multilayer films was best. It showed a wide linear range of 0.1-10mM glucose, with a remarkable sensitivity of 2.527 microA/mM, a detection limit of 6.7 microM estimated at a signal-to-noise ratio of 3 and fast response time (within 7s). Moreover, it exhibited good reproducibility, long-term stability and the negligible interferences of ascorbic acid, uric acid and acetaminophen. The study can provide a feasible approach on developing new kinds of oxidase-based amperometric biosensors, and can be used as an illustration for constructing various hybrid structures. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/17212983/Amperometric_glucose_biosensor_based_on_multilayer_films_via_layer_by_layer_self_assembly_of_multi_wall_carbon_nanotubes_gold_nanoparticles_and_glucose_oxidase_on_the_Pt_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(06)00565-3 DB - PRIME DP - Unbound Medicine ER -