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Time course of matrix metalloproteases and tissue inhibitors in bleomycin-induced pulmonary fibrosis.
Eur J Histochem. 2006 Oct-Dec; 50(4):317-25.EJ

Abstract

To investigate simultaneously localization and relative activity of MMPs during extracellular matrix (ECM) remodeling in bleomycin-induced pulmonary fibrosis in rat, we analyzed the time course of the expression, activity and/or concentration of gelatinases MMP-2 and MMP-9, collagenase MMP-1, matrylisin MMP-7, TIMP-1 and TIMP-2, both in alveolar space (cellular and extracellular compartments) and in lung tissue. MMP and TIMP expression was detected (immunohistochemistry) in lung tissue. MMP activity (zymography) and TIMP concentration (ELISA) were evaluated in lung tissue homogenate (LTH), BAL supernatant (BALs) and BAL cell pellet (BALp) 3, 7, 14, and 28 days after bleomycin intratracheal instillation. Immunohistochemistry showed an extensive MMP and TIMP expression from day 7 in a wide range of structural and inflammatory cells in treated rats. MMP-2 was present mainly in epithelia, MMP-9 in inflammatory cells. MMP-2 and MMP-9 activity was increased respectively in BAL fluid and BAL cells, with a peak at day 7. TIMP-1 and TIMP-2 concentration (ELISA) enhancement was delayed at day 14. In conclusion gelatinases and their inhibitors are significantly activated during bleomycin-induced pulmonary fibrosis. Marked changes in gelatinases activity are observed early in the alveolar compartment, with a prevailing extracellular activity of MMP-2 and a predominant intracellular distribution of MMP-9, while enzyme activity changes in lung parenchyma were less evident. In the repairing phase the reduction of gelatinases activity is synchronous with a peak of alveolar concentration of their inhibitors.

Authors+Show Affiliations

Department of Respiratory Diseases, IRCCS Policlinico San Matteo, University of Pavia, Italy. t.oggionni@smatteo.pv.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17213041

Citation

Oggionni, T, et al. "Time Course of Matrix Metalloproteases and Tissue Inhibitors in Bleomycin-induced Pulmonary Fibrosis." European Journal of Histochemistry : EJH, vol. 50, no. 4, 2006, pp. 317-25.
Oggionni T, Morbini P, Inghilleri S, et al. Time course of matrix metalloproteases and tissue inhibitors in bleomycin-induced pulmonary fibrosis. Eur J Histochem. 2006;50(4):317-25.
Oggionni, T., Morbini, P., Inghilleri, S., Palladini, G., Tozzi, R., Vitulo, P., Fenoglio, C., Perlini, S., & Pozzi, E. (2006). Time course of matrix metalloproteases and tissue inhibitors in bleomycin-induced pulmonary fibrosis. European Journal of Histochemistry : EJH, 50(4), 317-25.
Oggionni T, et al. Time Course of Matrix Metalloproteases and Tissue Inhibitors in Bleomycin-induced Pulmonary Fibrosis. Eur J Histochem. 2006 Oct-Dec;50(4):317-25. PubMed PMID: 17213041.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Time course of matrix metalloproteases and tissue inhibitors in bleomycin-induced pulmonary fibrosis. AU - Oggionni,T, AU - Morbini,P, AU - Inghilleri,S, AU - Palladini,G, AU - Tozzi,R, AU - Vitulo,P, AU - Fenoglio,C, AU - Perlini,S, AU - Pozzi,E, PY - 2007/1/11/pubmed PY - 2007/3/14/medline PY - 2007/1/11/entrez SP - 317 EP - 25 JF - European journal of histochemistry : EJH JO - Eur J Histochem VL - 50 IS - 4 N2 - To investigate simultaneously localization and relative activity of MMPs during extracellular matrix (ECM) remodeling in bleomycin-induced pulmonary fibrosis in rat, we analyzed the time course of the expression, activity and/or concentration of gelatinases MMP-2 and MMP-9, collagenase MMP-1, matrylisin MMP-7, TIMP-1 and TIMP-2, both in alveolar space (cellular and extracellular compartments) and in lung tissue. MMP and TIMP expression was detected (immunohistochemistry) in lung tissue. MMP activity (zymography) and TIMP concentration (ELISA) were evaluated in lung tissue homogenate (LTH), BAL supernatant (BALs) and BAL cell pellet (BALp) 3, 7, 14, and 28 days after bleomycin intratracheal instillation. Immunohistochemistry showed an extensive MMP and TIMP expression from day 7 in a wide range of structural and inflammatory cells in treated rats. MMP-2 was present mainly in epithelia, MMP-9 in inflammatory cells. MMP-2 and MMP-9 activity was increased respectively in BAL fluid and BAL cells, with a peak at day 7. TIMP-1 and TIMP-2 concentration (ELISA) enhancement was delayed at day 14. In conclusion gelatinases and their inhibitors are significantly activated during bleomycin-induced pulmonary fibrosis. Marked changes in gelatinases activity are observed early in the alveolar compartment, with a prevailing extracellular activity of MMP-2 and a predominant intracellular distribution of MMP-9, while enzyme activity changes in lung parenchyma were less evident. In the repairing phase the reduction of gelatinases activity is synchronous with a peak of alveolar concentration of their inhibitors. SN - 1121-760X UR - https://www.unboundmedicine.com/medline/citation/17213041/Time_course_of_matrix_metalloproteases_and_tissue_inhibitors_in_bleomycin_induced_pulmonary_fibrosis_ L2 - https://medlineplus.gov/pulmonaryfibrosis.html DB - PRIME DP - Unbound Medicine ER -