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Glucose biosensor based on immobilization of glucose oxidase in poly(o-aminophenol) film on polypyrrole-Pt nanocomposite modified glassy carbon electrode.
Biosens Bioelectron. 2007 Jun 15; 22(12):2898-905.BB

Abstract

Novel Pt nanoclusters embedded polypyrrole nanowires (PPy-Pt) composite was electrosynthesized on a glassy carbon electrode, denoted as PPy-Pt/GCE. A glucose biosensor was further fabricated based on immobilization of glucose oxidase (GOD) in an electropolymerized non-conducting poly(o-aminophenol) (POAP) film that was deposited on the PPy-Pt/GCE. The morphologies of the PPy nanowires and PPy-Pt nanocomposite were characterized by field emission scanning electron microscope (FE-SEM). Effect of experimental conditions involving the cycle numbers for POAP deposition and Pt nanoclusters deposition, applied potential used in glucose determination, temperature and pH value of the detection solution were investigated for optimization. The biosensor exhibited an excellent current response to glucose over a wide linear range from 1.5 x 10(-6) to 1.3 x 10(-2)M (r=0.9982) with a detection limit of 4.5 x 10(-7)M (s/n=3). Based on the combination of permselectivity of the POAP and the PPy films, the sensor had good anti-interference ability to ascorbic acid (AA), uric acid (UA) and acetaminophen. The apparent Michaelis-Menten constant (K(m)) and the maximum current density (I(m)) were estimated to be 23.9 mM and 378 microA/cm(2), respectively. In addition, the biosensor had also good sensitivity, stability and reproducibility.

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Authors+Show Affiliations

Li J
Department of Chemistry, University of Science and Technology of China, # 96 Jinzhai Rd., Hefei, Anhui 230026, PR China.
Lin X
No affiliation info available

MeSH

Biosensing TechniquesElectrochemistryElectrodesEnzymes, ImmobilizedGlucoseGlucose OxidaseHydrogen-Ion ConcentrationNanocompositesPlatinumPolymersPyrrolesTemperature

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17215117

Citation

Li, Jing, and Xiangqin Lin. "Glucose Biosensor Based On Immobilization of Glucose Oxidase in Poly(o-aminophenol) Film On polypyrrole-Pt Nanocomposite Modified Glassy Carbon Electrode." Biosensors & Bioelectronics, vol. 22, no. 12, 2007, pp. 2898-905.
Li J, Lin X. Glucose biosensor based on immobilization of glucose oxidase in poly(o-aminophenol) film on polypyrrole-Pt nanocomposite modified glassy carbon electrode. Biosens Bioelectron. 2007;22(12):2898-905.
Li, J., & Lin, X. (2007). Glucose biosensor based on immobilization of glucose oxidase in poly(o-aminophenol) film on polypyrrole-Pt nanocomposite modified glassy carbon electrode. Biosensors & Bioelectronics, 22(12), 2898-905.
Li J, Lin X. Glucose Biosensor Based On Immobilization of Glucose Oxidase in Poly(o-aminophenol) Film On polypyrrole-Pt Nanocomposite Modified Glassy Carbon Electrode. Biosens Bioelectron. 2007 Jun 15;22(12):2898-905. PubMed PMID: 17215117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glucose biosensor based on immobilization of glucose oxidase in poly(o-aminophenol) film on polypyrrole-Pt nanocomposite modified glassy carbon electrode. AU - Li,Jing, AU - Lin,Xiangqin, Y1 - 2007/01/09/ PY - 2006/08/22/received PY - 2006/11/30/revised PY - 2006/12/01/accepted PY - 2007/1/12/pubmed PY - 2007/7/27/medline PY - 2007/1/12/entrez SP - 2898 EP - 905 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 12 N2 - Novel Pt nanoclusters embedded polypyrrole nanowires (PPy-Pt) composite was electrosynthesized on a glassy carbon electrode, denoted as PPy-Pt/GCE. A glucose biosensor was further fabricated based on immobilization of glucose oxidase (GOD) in an electropolymerized non-conducting poly(o-aminophenol) (POAP) film that was deposited on the PPy-Pt/GCE. The morphologies of the PPy nanowires and PPy-Pt nanocomposite were characterized by field emission scanning electron microscope (FE-SEM). Effect of experimental conditions involving the cycle numbers for POAP deposition and Pt nanoclusters deposition, applied potential used in glucose determination, temperature and pH value of the detection solution were investigated for optimization. The biosensor exhibited an excellent current response to glucose over a wide linear range from 1.5 x 10(-6) to 1.3 x 10(-2)M (r=0.9982) with a detection limit of 4.5 x 10(-7)M (s/n=3). Based on the combination of permselectivity of the POAP and the PPy films, the sensor had good anti-interference ability to ascorbic acid (AA), uric acid (UA) and acetaminophen. The apparent Michaelis-Menten constant (K(m)) and the maximum current density (I(m)) were estimated to be 23.9 mM and 378 microA/cm(2), respectively. In addition, the biosensor had also good sensitivity, stability and reproducibility. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/17215117/Glucose_biosensor_based_on_immobilization_of_glucose_oxidase_in_poly_o_aminophenol__film_on_polypyrrole_Pt_nanocomposite_modified_glassy_carbon_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(06)00573-2 DB - PRIME DP - Unbound Medicine ER -