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Amperometric biosensor for the determination of creatine.
Anal Bioanal Chem. 2007 Mar; 387(5):1899-906.AB

Abstract

An amperometric biosensor for the determination of creatine was developed. The carbon rod electrode surface was coated with sarcosine oxidase (SOX) and creatine amidinohydrolase by cross-linking under glutaraldehyde vapour. The SOX from Arthrobacter sp. 1-1 N was purified and previously used for creation of a creatine biosensor. The natural SOX electron acceptor, oxygen, was replaced by an [Fe(CN)(6)](3-) /[ Fe(CN)(6)](4-) redox mediating system, which allowed amperometric detection of an analytical signal at +400-mV potential. The response time of the biosensor was less than 1 min. The biosensor showed a linear dependence of the signal vs. creatine concentration at physiological creatine concentration levels. The optimal pH in 0.1 M tris(hydroxymethyl)aminomethane (Tris)-HCl buffer was found to be at pH 8.0. The half-life of the biosensor was 8 days in 0.1 M Tris-HCl buffer (pH 8.0) at 20 degrees C. Principal scheme of consecutively followed catalytic reactions used to design a biosensor for the determination of creatine.

Authors+Show Affiliations

Ramanavicius A
Centre of Nanotechnology and Material Science, Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, 2006 Vilnius, Lithuania. arunas@imi.lt

MeSH

Biosensing TechniquesCreatineElectrochemistryEquipment DesignEquipment Failure AnalysisReproducibility of ResultsSarcosine OxidaseSensitivity and SpecificityUreohydrolases

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17221239

Citation

Ramanavicius, A. "Amperometric Biosensor for the Determination of Creatine." Analytical and Bioanalytical Chemistry, vol. 387, no. 5, 2007, pp. 1899-906.
Ramanavicius A. Amperometric biosensor for the determination of creatine. Anal Bioanal Chem. 2007;387(5):1899-906.
Ramanavicius, A. (2007). Amperometric biosensor for the determination of creatine. Analytical and Bioanalytical Chemistry, 387(5), 1899-906.
Ramanavicius A. Amperometric Biosensor for the Determination of Creatine. Anal Bioanal Chem. 2007;387(5):1899-906. PubMed PMID: 17221239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amperometric biosensor for the determination of creatine. A1 - Ramanavicius,A, Y1 - 2007/01/13/ PY - 2006/10/04/received PY - 2006/11/30/accepted PY - 2006/11/27/revised PY - 2007/1/16/pubmed PY - 2007/5/23/medline PY - 2007/1/16/entrez SP - 1899 EP - 906 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 387 IS - 5 N2 - An amperometric biosensor for the determination of creatine was developed. The carbon rod electrode surface was coated with sarcosine oxidase (SOX) and creatine amidinohydrolase by cross-linking under glutaraldehyde vapour. The SOX from Arthrobacter sp. 1-1 N was purified and previously used for creation of a creatine biosensor. The natural SOX electron acceptor, oxygen, was replaced by an [Fe(CN)(6)](3-) /[ Fe(CN)(6)](4-) redox mediating system, which allowed amperometric detection of an analytical signal at +400-mV potential. The response time of the biosensor was less than 1 min. The biosensor showed a linear dependence of the signal vs. creatine concentration at physiological creatine concentration levels. The optimal pH in 0.1 M tris(hydroxymethyl)aminomethane (Tris)-HCl buffer was found to be at pH 8.0. The half-life of the biosensor was 8 days in 0.1 M Tris-HCl buffer (pH 8.0) at 20 degrees C. Principal scheme of consecutively followed catalytic reactions used to design a biosensor for the determination of creatine. SN - 1618-2642 UR - https://www.unboundmedicine.com/medline/citation/17221239/Amperometric_biosensor_for_the_determination_of_creatine_ L2 - https://dx.doi.org/10.1007/s00216-006-1065-2 DB - PRIME DP - Unbound Medicine ER -