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Kallikrein 4 is a potential mediator of cellular interactions between cancer cells and osteoblasts in metastatic prostate cancer.
Prostate. 2007 Mar 01; 67(4):348-60.P

Abstract

BACKGROUND

Prostate cancer (PCa) and bone cell interactions are critical in the metastatic phase. Kallikrein 4 (KLK4/hK4) is expressed in both PCa and mineralized tissues. We determined if KLK4/hK4 expression was associated with, and influenced by, the bone environment of metastatic PCa.

METHODS

Immunohistochemistry, in vitro co-culture, cell migration, and attachment assays.

RESULTS

hK4 was localized to tumor cells and osteoblasts in bone metastases. KLK4/hK4 increased in LNCaP and PC3 cells co-cultured with SaOs2 cells; SaOs2 KLK4/hK4 was unchanged. Co-culture did not affect cell proliferation but altered alkaline phosphatase activity/mRNA levels in SaOs2 cells. KLK4-transfected PC3 cells had increased migration towards SaOs2 conditioned medium and greater attachment to the bone-matrix proteins, collagens I and IV.

CONCLUSIONS

hK4 expression and interaction with both tumor cells and osteoblasts suggests a role for hK4 in PCa bone metastasis. Whether this observation is unique to bone metastasis or reflects a role for hK4 in PCa metastasis generally is yet to be established.

Authors+Show Affiliations

Prostate Cancer Research Program, School of Life Sciences and Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17221837

Citation

Gao, Jin, et al. "Kallikrein 4 Is a Potential Mediator of Cellular Interactions Between Cancer Cells and Osteoblasts in Metastatic Prostate Cancer." The Prostate, vol. 67, no. 4, 2007, pp. 348-60.
Gao J, Collard RL, Bui L, et al. Kallikrein 4 is a potential mediator of cellular interactions between cancer cells and osteoblasts in metastatic prostate cancer. Prostate. 2007;67(4):348-60.
Gao, J., Collard, R. L., Bui, L., Herington, A. C., Nicol, D. L., & Clements, J. A. (2007). Kallikrein 4 is a potential mediator of cellular interactions between cancer cells and osteoblasts in metastatic prostate cancer. The Prostate, 67(4), 348-60.
Gao J, et al. Kallikrein 4 Is a Potential Mediator of Cellular Interactions Between Cancer Cells and Osteoblasts in Metastatic Prostate Cancer. Prostate. 2007 Mar 1;67(4):348-60. PubMed PMID: 17221837.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Kallikrein 4 is a potential mediator of cellular interactions between cancer cells and osteoblasts in metastatic prostate cancer. AU - Gao,Jin, AU - Collard,Rachael L, AU - Bui,Loan, AU - Herington,Adrian C, AU - Nicol,David L, AU - Clements,Judith A, PY - 2007/1/16/pubmed PY - 2007/4/19/medline PY - 2007/1/16/entrez SP - 348 EP - 60 JF - The Prostate JO - Prostate VL - 67 IS - 4 N2 - BACKGROUND: Prostate cancer (PCa) and bone cell interactions are critical in the metastatic phase. Kallikrein 4 (KLK4/hK4) is expressed in both PCa and mineralized tissues. We determined if KLK4/hK4 expression was associated with, and influenced by, the bone environment of metastatic PCa. METHODS: Immunohistochemistry, in vitro co-culture, cell migration, and attachment assays. RESULTS: hK4 was localized to tumor cells and osteoblasts in bone metastases. KLK4/hK4 increased in LNCaP and PC3 cells co-cultured with SaOs2 cells; SaOs2 KLK4/hK4 was unchanged. Co-culture did not affect cell proliferation but altered alkaline phosphatase activity/mRNA levels in SaOs2 cells. KLK4-transfected PC3 cells had increased migration towards SaOs2 conditioned medium and greater attachment to the bone-matrix proteins, collagens I and IV. CONCLUSIONS: hK4 expression and interaction with both tumor cells and osteoblasts suggests a role for hK4 in PCa bone metastasis. Whether this observation is unique to bone metastasis or reflects a role for hK4 in PCa metastasis generally is yet to be established. SN - 0270-4137 UR - https://www.unboundmedicine.com/medline/citation/17221837/Kallikrein_4_is_a_potential_mediator_of_cellular_interactions_between_cancer_cells_and_osteoblasts_in_metastatic_prostate_cancer_ L2 - https://doi.org/10.1002/pros.20465 DB - PRIME DP - Unbound Medicine ER -