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Direct extraction of penicillin G and derivatives from aqueous samples using a stoichiometrically imprinted polymer.
Anal Chem. 2007 Jan 15; 79(2):695-701.AC

Abstract

A molecularly imprinted polymer (MIP) prepared using penicillin G procaine salt as the template (PENGp) and a stoichiometric quantity of urea-based functional monomer to target the single oxyanionic species in the template molecule has been applied to the development of a molecularly imprinted solid-phase extraction (MISPE) procedure for the selective preconcentration of beta-lactam antibiotics (BLAs) from environmental water samples. Various parameters affecting the extraction efficiency of the polymer have been evaluated to achieve the selective preconcentration of the antibiotics from aqueous samples and to reduce nonspecific interactions. This resulted in an MISPE-HPLC method allowing the direct extraction of the analytes from the sample matrix with a selective wash using just 10% (v/v) organic solvent. On the basis of UV detection only, the method showed good recoveries and precision, ranging between 93% and 100% (RSD 3.8-8.9%, n = 3) for tap water and between 90% and 100% (RSD 4.2-9.1%, n = 3) for river water fortified with 30 or 60 microg L-1 (50 mL samples) penicillin G, penicillin V, nafcillin, oxacillin, cloxacillin, and dicloxacillin, suggesting that this MIP can be successfully applied to the direct preconcentration of BLAs in environmental water samples.

Authors+Show Affiliations

Urraca JL
Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense de Madrid, E-28040 Madrid, Spain.
Moreno-Bondi MC
No affiliation info available
Hall AJ
No affiliation info available
Sellergren B
No affiliation info available

MeSH

Chromatography, High Pressure LiquidEnvironmental MonitoringPenicillin GPolymersRiversWaterbeta-Lactams

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17222039

Citation

Urraca, Javier L., et al. "Direct Extraction of Penicillin G and Derivatives From Aqueous Samples Using a Stoichiometrically Imprinted Polymer." Analytical Chemistry, vol. 79, no. 2, 2007, pp. 695-701.
Urraca JL, Moreno-Bondi MC, Hall AJ, et al. Direct extraction of penicillin G and derivatives from aqueous samples using a stoichiometrically imprinted polymer. Anal Chem. 2007;79(2):695-701.
Urraca, J. L., Moreno-Bondi, M. C., Hall, A. J., & Sellergren, B. (2007). Direct extraction of penicillin G and derivatives from aqueous samples using a stoichiometrically imprinted polymer. Analytical Chemistry, 79(2), 695-701.
Urraca JL, et al. Direct Extraction of Penicillin G and Derivatives From Aqueous Samples Using a Stoichiometrically Imprinted Polymer. Anal Chem. 2007 Jan 15;79(2):695-701. PubMed PMID: 17222039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct extraction of penicillin G and derivatives from aqueous samples using a stoichiometrically imprinted polymer. AU - Urraca,Javier L, AU - Moreno-Bondi,María C, AU - Hall,Andrew J, AU - Sellergren,Börje, PY - 2007/1/16/pubmed PY - 2007/3/23/medline PY - 2007/1/16/entrez SP - 695 EP - 701 JF - Analytical chemistry JO - Anal Chem VL - 79 IS - 2 N2 - A molecularly imprinted polymer (MIP) prepared using penicillin G procaine salt as the template (PENGp) and a stoichiometric quantity of urea-based functional monomer to target the single oxyanionic species in the template molecule has been applied to the development of a molecularly imprinted solid-phase extraction (MISPE) procedure for the selective preconcentration of beta-lactam antibiotics (BLAs) from environmental water samples. Various parameters affecting the extraction efficiency of the polymer have been evaluated to achieve the selective preconcentration of the antibiotics from aqueous samples and to reduce nonspecific interactions. This resulted in an MISPE-HPLC method allowing the direct extraction of the analytes from the sample matrix with a selective wash using just 10% (v/v) organic solvent. On the basis of UV detection only, the method showed good recoveries and precision, ranging between 93% and 100% (RSD 3.8-8.9%, n = 3) for tap water and between 90% and 100% (RSD 4.2-9.1%, n = 3) for river water fortified with 30 or 60 microg L-1 (50 mL samples) penicillin G, penicillin V, nafcillin, oxacillin, cloxacillin, and dicloxacillin, suggesting that this MIP can be successfully applied to the direct preconcentration of BLAs in environmental water samples. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/17222039/Direct_extraction_of_penicillin_G_and_derivatives_from_aqueous_samples_using_a_stoichiometrically_imprinted_polymer_ L2 - https://doi.org/10.1021/ac061622r DB - PRIME DP - Unbound Medicine ER -