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Staphylococcus aureus-derived enterotoxins enhance house dust mite-induced patch test reactions in atopic dermatitis.
Exp Dermatol. 2007 Feb; 16(2):124-9.ED

Abstract

BACKGROUND

Up to 65% of Staphylococcus aureus strains isolated from the skin of patients with atopic dermatitis (AD) produce exotoxins with superantigenic properties that may also act as allergens leading to an induction of exotoxin-specific IgE antibodies. Staphylococcal enterotoxin B (SEB) applied epicutaneously in a concentration of 10 micro g/cm(2), i.e. 200 micro g/ml, under occlusion induces cutaneous inflammation in patients with AD and healthy individuals.

METHODS

We performed patch tests in 32 adult patients with AD using different concentrations (i.e. 2-200 micro g/ml) of SEA, SEB and house dust mite (HDM) extract (500 micro g/ml). Furthermore, the respective enterotoxins and HDM extract were applied simultaneously to the same patch test site. Specific IgE levels to SEA, SEB and HDM were measured with the CAP FEIA.

RESULTS

The rates of patch test reactions to SEA and SEB increased with rising enterotoxin concentrations. There were no differences in the rates of patch test reactions to SEA and SEB between patients sensitized to the corresponding enterotoxin and non-IgE-sensitized patients. The number of patch test reactions to the mixture of enterotoxin and HDM extract was higher than the number of patch test reactions to either the enterotoxins or HDM extract. We identified 11 patients with AD who reacted neither to the enterotoxins nor to HDM extract, but who showed patch test reactions to the mixture. These reactions were not predicted by the presence of enterotoxin- or HDM-specific IgE.

CONCLUSIONS

Colonization with exotoxin-producing S. aureus may influence the outcome of patch tests in patients with AD.

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Authors+Show Affiliations

Langer K
Department of Dermatology and Allergology, Hannover Medical School, Hannover, Germany. langer.katja@mh-hannover.de
Breuer K
No affiliation info available
Kapp A
No affiliation info available
Werfel T
No affiliation info available

MeSH

AdolescentAdultAnimalsDermatitis, AtopicEnterotoxinsFemaleHumansImmunoglobulin EMaleMiddle AgedPatch TestsPyroglyphidaeStaphylococcus aureus

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17222226

Citation

Langer, Katja, et al. "Staphylococcus Aureus-derived Enterotoxins Enhance House Dust Mite-induced Patch Test Reactions in Atopic Dermatitis." Experimental Dermatology, vol. 16, no. 2, 2007, pp. 124-9.
Langer K, Breuer K, Kapp A, et al. Staphylococcus aureus-derived enterotoxins enhance house dust mite-induced patch test reactions in atopic dermatitis. Exp Dermatol. 2007;16(2):124-9.
Langer, K., Breuer, K., Kapp, A., & Werfel, T. (2007). Staphylococcus aureus-derived enterotoxins enhance house dust mite-induced patch test reactions in atopic dermatitis. Experimental Dermatology, 16(2), 124-9.
Langer K, et al. Staphylococcus Aureus-derived Enterotoxins Enhance House Dust Mite-induced Patch Test Reactions in Atopic Dermatitis. Exp Dermatol. 2007;16(2):124-9. PubMed PMID: 17222226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Staphylococcus aureus-derived enterotoxins enhance house dust mite-induced patch test reactions in atopic dermatitis. AU - Langer,Katja, AU - Breuer,Kristine, AU - Kapp,Alexander, AU - Werfel,Thomas, PY - 2007/1/16/pubmed PY - 2007/5/1/medline PY - 2007/1/16/entrez SP - 124 EP - 9 JF - Experimental dermatology JO - Exp Dermatol VL - 16 IS - 2 N2 - BACKGROUND: Up to 65% of Staphylococcus aureus strains isolated from the skin of patients with atopic dermatitis (AD) produce exotoxins with superantigenic properties that may also act as allergens leading to an induction of exotoxin-specific IgE antibodies. Staphylococcal enterotoxin B (SEB) applied epicutaneously in a concentration of 10 micro g/cm(2), i.e. 200 micro g/ml, under occlusion induces cutaneous inflammation in patients with AD and healthy individuals. METHODS: We performed patch tests in 32 adult patients with AD using different concentrations (i.e. 2-200 micro g/ml) of SEA, SEB and house dust mite (HDM) extract (500 micro g/ml). Furthermore, the respective enterotoxins and HDM extract were applied simultaneously to the same patch test site. Specific IgE levels to SEA, SEB and HDM were measured with the CAP FEIA. RESULTS: The rates of patch test reactions to SEA and SEB increased with rising enterotoxin concentrations. There were no differences in the rates of patch test reactions to SEA and SEB between patients sensitized to the corresponding enterotoxin and non-IgE-sensitized patients. The number of patch test reactions to the mixture of enterotoxin and HDM extract was higher than the number of patch test reactions to either the enterotoxins or HDM extract. We identified 11 patients with AD who reacted neither to the enterotoxins nor to HDM extract, but who showed patch test reactions to the mixture. These reactions were not predicted by the presence of enterotoxin- or HDM-specific IgE. CONCLUSIONS: Colonization with exotoxin-producing S. aureus may influence the outcome of patch tests in patients with AD. SN - 0906-6705 UR - https://www.unboundmedicine.com/medline/citation/17222226/Staphylococcus_aureus_derived_enterotoxins_enhance_house_dust_mite_induced_patch_test_reactions_in_atopic_dermatitis_ L2 - https://doi.org/10.1111/j.1600-0625.2006.00523.x DB - PRIME DP - Unbound Medicine ER -