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Selective hair analysis of nicotine by molecular imprinted solid-phase extraction: an application for evaluating tobacco smoke exposure.
Food Chem Toxicol. 2007 Jun; 45(6):896-903.FC

Abstract

A method using a molecularly imprinted polymer (MIP) as the selective sorbent for solid-phase extraction (SPE) has been developed. Its application to the assay of hairy nicotine level among smokers and non-smokers with high-performance liquid chromatography (HPLC) and evaluation of exposures to the environmental tobacco smoke (ETS) were validated. The MIP was synthesized using nicotine as the template molecule and methacrylic acid (MAA) as the functional monomer. This MIP-SPE method provided inherent selectivity and a sensitive response to nicotine with a detection limit of 0.2 ng/ml hair at a signal-to-noise ratio of 3:1 and the limit of quantification was 0.5 ng/ml. The linearity was assessed in the range of 0.5-80 ng/ml hair, with a coefficient (r(2)) greater than 0.987. The amounts of nicotine determined in smokers and non-smokers hair were in the range of 5.1-69.5 ng/mg hair and 0.50-9.3 ng/mg hair, respectively. The reported measures of ETS exposure were significantly associated with hairy nicotine levels. This assay of nicotine in hair using MISPE provided a very selective and reliable method for the evaluation of the exposure to tobacco smoke.

Authors+Show Affiliations

Yang J
Department of Chemistry, University of Science and Technology of China, Hefei 230026, PR China.
Hu Y
No affiliation info available
Cai JB
No affiliation info available
Zhu XL
No affiliation info available
Su QD
No affiliation info available
Hu YQ
No affiliation info available
Liang FX
No affiliation info available

MeSH

CotinineFemaleHairHumansMaleNicotineSolid Phase ExtractionTobacco Smoke Pollution

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17222493

Citation

Yang, J, et al. "Selective Hair Analysis of Nicotine By Molecular Imprinted Solid-phase Extraction: an Application for Evaluating Tobacco Smoke Exposure." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 45, no. 6, 2007, pp. 896-903.
Yang J, Hu Y, Cai JB, et al. Selective hair analysis of nicotine by molecular imprinted solid-phase extraction: an application for evaluating tobacco smoke exposure. Food Chem Toxicol. 2007;45(6):896-903.
Yang, J., Hu, Y., Cai, J. B., Zhu, X. L., Su, Q. D., Hu, Y. Q., & Liang, F. X. (2007). Selective hair analysis of nicotine by molecular imprinted solid-phase extraction: an application for evaluating tobacco smoke exposure. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 45(6), 896-903.
Yang J, et al. Selective Hair Analysis of Nicotine By Molecular Imprinted Solid-phase Extraction: an Application for Evaluating Tobacco Smoke Exposure. Food Chem Toxicol. 2007;45(6):896-903. PubMed PMID: 17222493.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective hair analysis of nicotine by molecular imprinted solid-phase extraction: an application for evaluating tobacco smoke exposure. AU - Yang,J, AU - Hu,Y, AU - Cai,J B, AU - Zhu,X L, AU - Su,Q D, AU - Hu,Y Q, AU - Liang,F X, Y1 - 2006/11/26/ PY - 2005/11/15/received PY - 2006/05/18/revised PY - 2006/11/20/accepted PY - 2007/1/16/pubmed PY - 2007/7/25/medline PY - 2007/1/16/entrez SP - 896 EP - 903 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 45 IS - 6 N2 - A method using a molecularly imprinted polymer (MIP) as the selective sorbent for solid-phase extraction (SPE) has been developed. Its application to the assay of hairy nicotine level among smokers and non-smokers with high-performance liquid chromatography (HPLC) and evaluation of exposures to the environmental tobacco smoke (ETS) were validated. The MIP was synthesized using nicotine as the template molecule and methacrylic acid (MAA) as the functional monomer. This MIP-SPE method provided inherent selectivity and a sensitive response to nicotine with a detection limit of 0.2 ng/ml hair at a signal-to-noise ratio of 3:1 and the limit of quantification was 0.5 ng/ml. The linearity was assessed in the range of 0.5-80 ng/ml hair, with a coefficient (r(2)) greater than 0.987. The amounts of nicotine determined in smokers and non-smokers hair were in the range of 5.1-69.5 ng/mg hair and 0.50-9.3 ng/mg hair, respectively. The reported measures of ETS exposure were significantly associated with hairy nicotine levels. This assay of nicotine in hair using MISPE provided a very selective and reliable method for the evaluation of the exposure to tobacco smoke. SN - 0278-6915 UR - https://www.unboundmedicine.com/medline/citation/17222493/Selective_hair_analysis_of_nicotine_by_molecular_imprinted_solid_phase_extraction:_an_application_for_evaluating_tobacco_smoke_exposure_ DB - PRIME DP - Unbound Medicine ER -