TY - JOUR T1 - High performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-MS/ESI) method for simultaneous determination of venlafaxine and its three metabolites in human plasma. AU - Liu,Wen, AU - Cai,Hua-Lin, AU - Li,Huan-de, Y1 - 2006/12/21/ PY - 2006/08/10/received PY - 2006/12/08/revised PY - 2006/12/12/accepted PY - 2007/1/16/pubmed PY - 2007/7/25/medline PY - 2007/1/16/entrez SP - 405 EP - 11 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 850 IS - 1-2 N2 - A high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-MS/ESI) method for simultaneous determination of venlafaxine (VEN) and its three metabolites O-desmethylvenlafaxine (ODV), N-desmethylvenlafaxine (NDV) and N,O-didesmethylvenlafaxine (DDV) in human plasma has been developed and validated. Estazolam was used as the internal standard. The compounds and internal standard were extracted from plasma by a liquid-liquid extraction. The HPLC separation of the analytes was performed on a Thermo BDS HYPERSIL C18 (250 mm x 4.6 mm, 5 microm, USA) column, using a gradient elution program with solvents constituted of water (ammonium acetate: 30 mmol/l, formic acid 2.6 mmol/l and trifluoroacetic acid 0.13 mmol/l) and acetonitrile (60:40, V/V) at a flow-rate of 1.0 ml/min. All of the analytes were eluted within 6 min. The compounds were ionized in the electrospray ionization (ESI) ion source of the mass spectrometer and were detected in the selected ion recording (SIR) mode. Calibration curves in spiked whole blood were linear from 4.0-700 ng/ml, 2.0-900 ng/ml, 3.0-800 ng/ml and 2.0-700 ng/ml for VEN, ODV, NDV and DDV, respectively, all of them with coefficients of determination above 0.9991. The average extraction recoveries for all the four analytes were above 77%. The methodology recoveries were higher than 91%. The limits of detection were 0.4, 0.2, 0.3, and 0.2 ng/ml for VEN, ODV, NDV and DDV, respectively. The intra- and inter-day variation coefficients were less than 11%. The method is accurate, sensitive and reliable for the pharmacokinetic study of venlafaxine as well as therapeutic drug monitoring (TDM). SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/17222591/High_performance_liquid_chromatography_electrospray_ionization_mass_spectrometry__HPLC_MS/ESI__method_for_simultaneous_determination_of_venlafaxine_and_its_three_metabolites_in_human_plasma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(06)01030-0 DB - PRIME DP - Unbound Medicine ER -